new-kit-box new-kit-box Z6010-reliaprep-rna-cell-miniprep-system-10-preps z6011-reliaprep-rna-cell-miniprep-system-50-preps z6012-reliaprep-rna-cell-miniprep-system-250-preps Z6110-reliaprep-rna-tissue-miniprep-system-10-preps Z6111-reliaprep-rna-tissue-miniprep-system-50-preps Z6112-reliaprep-rna-tissue-miniprep-system-250-preps

Efficiently Purify RNA from as Few as 100 Cells or 0.25mg of Tissue

  • One kit, two workflows: A single unified solution for both cell and tissue RNA extractions
  • Simple and fast: Extract high-quality RNA in 30 minutes with a streamlined, easy-to-use protocol
  • No phenol/chloroform or β-mercaptoethanol required

New: Cells and Tissue

  Catalog No. Product Name   Size Price Qty  
Z6014 ReliaPrep™ RNA Miniprep System View Specifications 50 preps     Please Enquire
Z6015 ReliaPrep™ RNA Miniprep System View Specifications 250 preps     Please Enquire

Early Access = This product is available under our Early Access program - Learn More

Catalog (FT) = This product is available under our Catalog (FT) program - Learn More

Cells*

  Catalog No. Product Name   Size Price Qty  
Z6010 ReliaPrep™ RNA Cell Miniprep System View Specifications 10 preps     Please Enquire
Z6011 ReliaPrep™ RNA Cell Miniprep System View Specifications 50 preps     Please Enquire
Z6012 ReliaPrep™ RNA Cell Miniprep System View Specifications 250 preps     Please Enquire

Early Access = This product is available under our Early Access program - Learn More

Catalog (FT) = This product is available under our Catalog (FT) program - Learn More

Tissue*

  Catalog No. Product Name   Size Price Qty  
Z6110 ReliaPrep™ RNA Tissue Miniprep System View Specifications 10 preps     Please Enquire
Z6111 ReliaPrep™ RNA Tissue Miniprep System View Specifications 50 preps     Please Enquire
Z6112 ReliaPrep™ RNA Tissue Miniprep System View Specifications 250 preps     Please Enquire

Early Access = This product is available under our Early Access program - Learn More

Catalog (FT) = This product is available under our Catalog (FT) program - Learn More

*The ReliaPrep™ RNA Cell Miniprep Systems (Cat.# Z6010, Z6011, Z6012) are for cell samples only. The ReliaPrep™ RNA Tissue Miniprep Systems (Cat.# Z6110, Z6111, Z6112) are for tissue samples only. These are legacy configurations of the ReliaPrep™ RNA Miniprep System, which will be discontinued shortly and replaced by the consolidated ReliaPrep™ Miniprep Systems Cat.# Z6014, Z6015 listed above.

Overview
Protocols
Specifications
Resources
Related Products

貴重な細胞や組織サンプルより高純度なRNAを高濃度に精製

ReliaPrep™ RNA Miniprep Systemは培養細胞または組織からインタクトなTotal RNAの精製を約30分程度で迅速、シンプルに行うためのシステムです。特殊なカラム/結合マトリクスにより非常に少量のサンプルより効率的にRNAを捕捉することができ、溶出量を最小限に抑えることができます(15µl以下)。このメンブレンベースの精製システムは1回の精製で5×106個の細胞または0.25~20mgの組織を処理することができます。本システムにはミニカラム上で行うDNase処理ステップが含まれており、下流のアッセイを阻害する物質を効率よく除去することができます。フェノール/クロロホルム抽出やエタノール沈殿を行わずに精製することができ、用途に合わせてRNAをさらに精製したり濃縮する必要がありません。

  • 100個程度の少ない細胞あるいは0.25~20mg程度の少量の組織からでもRNAを精製可能
  • 阻害剤を含まない純度の高いRNAを精製
  • 15µl以下に濃縮されたRNAを溶出
  • 迅速な30分間プロトコル

  • 様々な下流の用途(定量など)に適したRNAの精製

【簡易マニュアル】日本語:cell,【簡易マニュアル】日本語:tissue
※日本語マニュアルは英語版を翻訳したものですが、常に更新されるものではありません。最新のマニュアルについては英語版(”プロトコール・記事等”タブ→“プロトコール”)をご覧ください。
【Q&A】ReliaPrep RNA Miniprep Systems   【アプリケーション】植物からのRNA精製
Schematic of the ReliaPrep™ Miniprep RNA purification workflow.

RNA from Cells

高収量

ReliaPrep™ Cell RNA Miniprep Systemは 100 から 1 x 106 個の HeLa 細胞より他社競合製品よりも多くのRNAを効率的に精製(右グラフ)。4種の細胞株それぞれ100,000細胞からの RNA 収量(下グラフ)。ReliaPrep™ System のその他のパフォーマンスデータについては Application Note をご覧ください。

Bar graph showing strong, consistent RNA yields across a wide range of cultured cell inputs using the ReliaPrep™ RNA Miniprep System. Yields are comparable to, or exceeding, those of the leading competitor.
Bar graph showing RNA recovery from 100,000 cells across four cell lines using the ReliaPrep™ RNA Miniprep System, demonstrating robust performance and reliable yield even with low-input samples.

RNA from Tissue

組織から精製した RNA の収量および純度

ReliaPrep™ RNA Tissue Miniprep System を用いて線維性および非線維性のマウス組織より精製した標準的なRNA 収量。RNA は様々なマウス組織 10mg より精製。収量および純度は吸光光度計 Nanodrop® 1000 を用いて測定した。

ReliaPrep™ RNA Tissue Miniprep System により得られたその他のデータについては Application Note をご覧ください。

Bar graphs showing that the ReliaPrep™ RNA Miniprep System efficiently isolates RNA from a broad range of mouse tissues, including both fibrous and nonfibrous sample types. The data shown represent RNA yield from 10mg tissue samples, quantified using absorbance spectroscopy.

Specifications

Catalog Number:

選択製品の構成品内容

Item Part # Size Available Separately

Column Wash Solution (CWE)

A254B 1 × 5ml

BL Buffer

Z103B 1 × 32.5ml

Lysis Buffer (LBA)

Z106B 1 × 30ml
RNA Wash Solution (RWA) Z309E 1 × 35ml View Product

MnCl2, 0.09M

Z318A 1 × 250μl

RNA Dilution Buffer (RDB)

Z372A 1 × 44ml

Collection Tubes

A130A 2 × 50/pk

ReliaPrep™ Minicolumns

A207B 1 × 50/pack

1-Thioglycerol

A208B 2 × 900μl

Nuclease-Free Water

P119E 1 × 13ml View Product

Elution Tubes

Z200C 1 × 50/pk

Yellow Core Buffer

Z317A 1 × 2.5ml

DNase I (lyophilized)

Z358A 1 × 1 vial View Product

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

選択製品の構成品内容

Item Part # Size Available Separately

Column Wash Solution (CWE)

A254C 1 × 24ml
BL Buffer Z103C 1 × 150ml

Lysis Buffer (LBA)

Z106C 1 × 150ml

RNA Wash Solution (RWA)

Z309D 1 × 206ml View Product

Yellow Core Buffer

Z317C 1 × 11ml

RNA Dilution Buffer (RDB)

Z372B 1 × 220ml

Collection Tubes

A130B 2 × 250/pk

ReliaPrep™ Minicolumns

A207B 5 × 50/pack

1-Thioglycerol

A208B 4 × 900μl

Nuclease-Free Water

P119E 1 × 13ml View Product

Elution Tubes

Z200C 5 × 50/pk

MnCl2, 0.09M

Z318B 2 × 750μl

DNase I (lyophilized)

Z358A 5 × 1 vial View Product

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

選択製品の構成品内容

Item Part # Size Available Separately

Collection Tubes

A130C 2 × 10/pk
ReliaPrep™ Minicolumns A207A 1 × 10/pack
1-Thioglycerol A208A 1 × 75μl

Column Wash Solution (CWE)

A254A 1 × 1ml

Nuclease-Free Water

P119A 1 × 1,250μl View Product

BL Buffer

Z103A 2 × 3.25ml

Elution Tubes

Z200B 1 × 25/pk

RNA Wash Solution (RWA)

Z309B 1 × 11.8ml View Product
Yellow Core Buffer Z317B 1 × 300μl

MnCl2, 0.09M

Z318A 1 × 250μl

DNase I (lyophilized)

Z358B 1 × 1 vial View Product

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

選択製品の構成品内容

Item Part # Size Available Separately

Collection Tubes

A130A 2 × 50/pk

ReliaPrep™ Minicolumns

A207B 1 × 50/pack

1-Thioglycerol

A208B 1 × 900μl

Column Wash Solution (CWE)

A254B 1 × 5ml

Nuclease-Free Water

P119E 1 × 13ml View Product

BL Buffer

Z103B 1 × 32.5ml

Elution Tubes

Z200C 1 × 50/pk
RNA Wash Solution (RWA) Z309E 1 × 35ml View Product

Yellow Core Buffer

Z317A 1 × 2.5ml

MnCl2, 0.09M

Z318A 1 × 250μl

DNase I (lyophilized)

Z358A 1 × 1 vial View Product

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

選択製品の構成品内容

Item Part # Size Available Separately

Collection Tubes

A130B 2 × 250/pk

ReliaPrep™ Minicolumns

A207B 5 × 50/pack

1-Thioglycerol

A208B 2 × 900μl

Column Wash Solution (CWE)

A254C 1 × 24ml

Nuclease-Free Water

P119E 1 × 13ml View Product
BL Buffer Z103C 1 × 150ml

Elution Tubes

Z200C 5 × 50/pk

RNA Wash Solution (RWA)

Z309D 1 × 206ml View Product

Yellow Core Buffer

Z317C 1 × 11ml

MnCl2, 0.09M

Z318B 2 × 750μl

DNase I (lyophilized)

Z358A 3 × 1 vial View Product

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

選択製品の構成品内容

Item Part # Size Available Separately

Collection Tubes

A130C 2 × 10/pk
ReliaPrep™ Minicolumns A207A 1 × 10/pack
1-Thioglycerol A208A 2 × 75μl

Column Wash Solution (CWE)

A254A 1 × 1ml

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Lysis Buffer (LBA)

Z106A 1 × 6ml

Elution Tubes

Z200B 1 × 25/pk

RNA Wash Solution (RWA)

Z309B 1 × 11.8ml View Product
Yellow Core Buffer Z317B 1 × 300μl

MnCl2, 0.09M

Z318A 1 × 250μl

DNase I (lyophilized)

Z358B 1 × 1 vial View Product

RNA Dilution Buffer (RDB)

Z372C 1 × 9ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

選択製品の構成品内容

Item Part # Size Available Separately

Collection Tubes

A130A 2 × 50/pk

ReliaPrep™ Minicolumns

A207B 1 × 50/pack

1-Thioglycerol

A208B 2 × 900μl

Column Wash Solution (CWE)

A254B 1 × 5ml

Nuclease-Free Water

P119E 1 × 13ml View Product

Lysis Buffer (LBA)

Z106B 1 × 30ml

Elution Tubes

Z200C 1 × 50/pk
RNA Wash Solution (RWA) Z309E 1 × 35ml View Product

Yellow Core Buffer

Z317A 1 × 2.5ml

MnCl2, 0.09M

Z318A 1 × 250μl

DNase I (lyophilized)

Z358A 1 × 1 vial View Product

RNA Dilution Buffer (RDB)

Z372A 1 × 44ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

選択製品の構成品内容

Item Part # Size Available Separately

Collection Tubes

A130B 2 × 250/pk

ReliaPrep™ Minicolumns

A207B 5 × 50/pack

1-Thioglycerol

A208B 4 × 900μl

Column Wash Solution (CWE)

A254C 1 × 24ml

Nuclease-Free Water

P119E 1 × 13ml View Product

Lysis Buffer (LBA)

Z106C 1 × 150ml

Elution Tubes

Z200C 5 × 50/pk

RNA Wash Solution (RWA)

Z309D 1 × 206ml View Product

Yellow Core Buffer

Z317C 1 × 11ml

MnCl2, 0.09M

Z318B 2 × 750μl

DNase I (lyophilized)

Z358A 3 × 1 vial View Product

RNA Dilution Buffer (RDB)

Z372B 1 × 220ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD