Proteinase K (PK) Solution

Proteinase K, produced by the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage activity. It cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological samples. It has been purified to remove RNase and DNase activities. The stability of Proteinase K in urea and SDS and its ability to digest native proteins make it useful for a variety of applications including preparation of chromosomal DNA for pulsed-field gel electrophoresis, protein fingerprinting and removal of nucleases from preparations of DNA and RNA. A typical working concentration for Proteinase K is 50–100μg/ml.

Formulation: Proteinase K (PK) Solution is supplied at a concentration of 20mg/ml in 10mM Tris-HCl (pH 7.5), 1mM calcium chloride and 50% glycerol.

Download our Green Sheet to learn more about the features that make this an Environmentally Preferable Product:

• 安定性: 0.5%SDSまたは1%Triton®X-100、およびpH 4.3-12.0で活性を有し、最大60℃まで80%以上の活性を保持
• 使いやすい: 室温で安定な溶液タイプなので、再懸濁や使用前の融解が不要

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