5X AmpSolution™ Reagent, PunchSolution™ Kit and SwabSolution™ Kit
Rapid, Simple Preparation Methods for Swabs and Punches
- For processing of single-source reference, database and paternity samples where purification is unnecessary
- Save 2–4 hours per plate of samples
- Compatibility with most PowerPlex® Systems
Catalog Number:
Choose a product
Size
Catalog Number: DM1231
Catalog Number: DC9271
Catalog Number: DC8271
5X AmpSolution™ Reagent
The 5X AmpSolution™ Reagent streamlines the processing of swabs and punches for PowerPlex® STR analysis without compromising your results. While direct amplification from FTA® cards is transforming workflows in databasing and paternity testing labs, buccal swabs and non FTA cards are not ideal substrates for direct amplification.
Additionally, the AmpSolution™ Reagent enables direct amplification of DNA from unwashed FTA® punches, nonFTA punches and swabs from traditional PowerPlex® kits that were not originally intended for that application, minimizing the need to validate a new system to perform direct amplification.
Buccal Samples on FTA® Punches
Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.
SwabSolution™ Kit
The SwabSolution™ Kit is used for rapid processing of swabs for STR analysis using PowerPlex® Systems. The SwabSolution™ Kit contains SwabSolution™ Reagent, which is used to generate a buccal swab extract that is added to the PowerPlex® System reaction. In addition, the SwabSolution™ Kit contains the 5X AmpSolution™ Reagent, which enables direct amplification of DNA from swabs using certain PowerPlex® Systems.
Buccal Swab Workflow
PunchSolution™ Kit
PunchSolution™ Kit works in conjunction with PowerPlex® Systems to provide rapid processing of punches from nonFTA storage cards (S&S 903, Bode Buccal Collector™ device, etc.) and robust amplification profiles. The PunchSolution™ Kit contains PunchSolution™ Reagent, which is used to pretreat nonFTA punches prior to addition of the PowerPlex® PCR amplification mix. In addition, the PunchSolution™ Kit contains the 5X AmpSolution™ Reagent, which enables direct amplification of DNA from punches.
The PunchSolution™ Kit offers a simple sample preparation process to simplify analysis. At 10μl, the volume of PunchSolution™ Reagent allows easier punch handling than other pretreatment products that only use 2μl of solution. Higher quality, easier-to-interpret profiles are obtained, compared to other methods for processing nonFTA punches.
Blood on S&S 903
One 1.2mm blood punch on S&S903 card was pretreated with PunchSolution™ Reagent as recommended in the PunchSolution™ Kit Technical Manual TMD038. Amplification was performed using the PowerPlex® 18D System. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection).
Rapid Processing Solutions for PowerPlex® Systems
The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® Fusion, 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.
Which Product is Right for Me?
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
| 5X AmpSolution™ Reagent | DM123A | 1 × 500μl | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
| PunchSolution™ Reagent | DM121A | 1 × 1ml | |
| 5X AmpSolution™ Reagent | DM123A | 1 × 500μl | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
| SwabSolution™ Reagent | DM122A | 1 × 100ml | |
| 5X AmpSolution™ Reagent | DM123A | 1 × 500μl | View Product |
Resources
No related resources found
Related Products
関連製品
PowerPlex® Fusion 6C System
27ローカス同時増幅の6色蛍光検出システム
DC2705, DC2720, DC2780
PowerPlex® Y23 System
23のY-STRローカス増幅用5色蛍光マルチプレックスPCR
DC2305, DC2320, DM6571
PowerPlex® 21 System
ヒト個人識別用マルチプレックスSTRシステム
DC8902, DC8942
PowerPlex® ESX 17 and ESI 17 Fast Systems
ENFSI推奨のSTRローカスを同時増幅、5色蛍光で検出
DC1710, DC1711, DC1720, DC1721, DC1731, DC1730
