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AMV Reverse Transcriptase

Part Numbers: M5101, M5108, M9004

M5101_AMV-Reverse-Transcriptase--300u_3
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強い二次構造を形成する鋳型でも効率的に逆転写

  • DNA、RNA または RNA:DNA ハイブリッドの鋳型よりDNA合成
  • 活性化にはマグネシウム、マンガンおよびプライマーが必要
  • より高い反応温度でも安定 (37–58°C)

Size

選択製品のカタログ番号: M5101

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AMV Reverse Transcriptase
300u
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Reverse Transcriptase with Stability at Higher Temperatures

AMV (Avian Myeloblastosis Virus) Reverse Transcriptaseは、トリ骨髄芽球症ウイルスから発見された逆転写酵素で、DNA, RNA, DNA:RNAハイブリッドをテンプレートとするDNAポリメラーゼでもあります。活性化にはプライマー（RNAプライマーよりもDNAプライマーが効果的）、Mg2+またはMn2+が必要です。本酵素は内在性のリボヌクレアーゼH活性を示します。非イオン性界面活性剤とスルフヒドリル化合物はin vitroでの酵素活性を安定化します。

  • 高濃度: Cat.# M9004 は、 AMV RT (20–25units/μl) 600ユニットを含みます 
  • 5X Reaction Bufferを添付：250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 50mM MgCl2, 2.5mM spermidine, 50mM DTT
  • AMV RTは比較的高温（37～58°C）でも安定なので二次構造を形成したRNAの逆転写に最適です
  • 75°C, 15分で不活性化します。

  • ファーストストランドcDNA合成
  • プライマー伸長反応およびRNAシークエンシング
  • 標識ハイブリダイゼーションプローブの調製
  • Taq DNA Polymeraseを用いた50µl PCRの場合、RT反応液10µlまでをそのまま使用可能。GoTaq® DNA PolymeraseおよびPCR Master MixならRT反応25µlまでをそのまま使用可能。

 【Q&A】AMV Reverse Transcriptase
  1. Kacian, D.L. (1977) Meth. Virol. 6, 143.
  2. Mierendorf, R.C. and Pfeffer, D. (1987) Meth. Enzymol. 152, 563–6.
View AMV Citations

プロトコール

Complete Protocol

製品仕様

You are viewing: M5101 Change Configuration

選択製品の構成品内容

Item Part # Size

AMV Reverse Transcriptase

 M510A 1 × 300u
AMV RT 5X Buffer M515A 1 × 500μl

SDS

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分析証明書

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使用制限

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

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製品仕様

You are viewing: M5108 Change Configuration

選択製品の構成品内容

Item Part # Size

AMV Reverse Transcriptase

 M510F 1 × 1,000u
AMV RT 5X Buffer M515A 3 × 500μl

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

保存条件

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製品仕様

You are viewing: M9004 Change Configuration

選択製品の構成品内容

Item Part # Size
AMV RT 5X Buffer M515A 1 × 500μl

AMV Reverse Transcriptase (HC)

 M900A 1 × 600u

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

保存条件

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技術資料

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