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ImProm-II™ Reverse Transcription System

Part Numbers: A3800

ImProm II Reverse Transcription System 100 reactions
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8.9kb までの鋳型 RNA を逆転写

Size

選択製品のカタログ番号: A3800

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ImProm-II™ Reverse Transcription System
100 reactions
Your price:

Complete System for First-Strand cDNA Synthesis of Long or Rare Messages

ImProm-ll™ Reverse Transcription System はPCR用の完全長第1鎖cDNAを効率的に合成します。このシステムは長いまたは希少なpoly(A) mRNA、Total RNAや合成RNAを逆転写することができます。最適化した反応バッファーや強力なImProm-ll™ Reverse Transcriptaseは、単独のアプリケーションや遺伝子特異的なターゲットを増幅するために調整されています。逆転写反応液1～20µlであればプロメガのTaq DNA Polymeraseを用いてダイレクトに増幅することができます（連続または非連続）。また、蛍光色素などで標識されたヌクレオチドの取込み効率に優れ、マイクロアレイ用のプローブ合成にも最適です。


  • 完全長cDNA合成：8.9Kbまでの鋳型RNAを逆転写
  • 優れた取込み効率：標準的、蛍光標識またはアミノアリル修飾したヌクレオチドの組込み
  • 使い易い：効率的な逆転写に必要な全ての試薬が含まれる
  • スケール自在：1～20µlのRT反応液を次のPCR反応に使用

さらに知りたい方は、プロトコール＆ガイド(英語)をご覧ください。


【簡易マニュアル】日本語：ImProm-II Reverse Transcriptase
※日本語マニュアルは英語版を翻訳したものですが、常に更新されるものではありません。最新のマニュアルについては英語版（”プロトコール･記事等”タブ→“プロトコール”）をご覧ください。
【パフォーマンス】RT-PCR に最適な新しい逆転写酵素 ≪ImProm-IITM Reverse Transcriptase≫

プロトコール

Complete Protocol

製品仕様

選択製品の構成品内容

Item Part # Size Concentration Available Separately

Upstream Control Primer

 A109B 1 × 100μl 15μM

Downstream Control Primer

 A110B 1 × 100μl 15μM

MgCl2

 A351H 1 × 1.2ml 25mM View Product

Oligo(dT)15 Primer

 C110B 1 × 50μg 500μg/ml View Product

dNTP Mix

 C114B 1 × 320μl 10mM View Product

Random Primers

 C118B 1 × 50μg View Product

1.2kb Kanamycin Positive Control RNA

 C138A 1 × 5μg 0.5mg/ml View Product

ImProm-II™ 5X Reaction Buffer

 M289A 1 × 600μl

ImProm-II™ Reverse Transcriptase

 M314A 1 × 100μl

Recombinant RNasin® Ribonuclease Inhibitor

 N251A 1 × 2,500u 40u/μl View Product

Nuclease-Free Water

 P119A 2 × 1,250μl View Product

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

See Protocol for detailed storage recommendations.

技術資料

サイテーション

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