M-MLV Reverse Transcriptase, RNase H Minus
Helpful for Preparing Long cDNAs and Libraries with a High Percentage of Full-Length cDNA
- Supplied at a concentration of 100–200u/μl
Catalog Number:
Size
Catalog Number: M5301
First-Strand cDNA Synthesis with Long mRNA Templates
本酵素は、M-MLVから遺伝子操作技術によりリボヌクくレアーゼH活性に関与する部分を欠失変異させたRNA依存型DNAポリメラーゼで、長いmRNA (>5kb)からのcDNA合成に適しています。
- 5X Reaction Buffer添付：250mM Tris-HCl (pH 8.3 at 25℃), 375mM KCl, 15mM MgCl2, 50mM DTT
- 70℃, 10分で不活性化します
- 第1鎖cDNA合成
- プライマー伸長反応
【Ｑ＆Ａ】M-MLV Reverse Transcriptase, RNase H Minus
- Roth, M.J. et al. (1985) J. Biol. Chem. 260, 9326–35.
- Tanese, N. and Goff, S.P. (1988) Proc. Natl. Acad. Sci. USA 85, 1777–81.
Technical Details
Storage Buffer: 20mM Tris-HCl (pH 7.5 at 25°C), 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease.
Unit Definition: One unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 7mM MgCl2, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [3H]dTTP, 0.025mM oligo(dT), 0.25mM poly(A) and 0.01% NP-40.
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
|
M-MLV Reverse Transcriptase, RNase H Minus |
M530A | 1 × 10,000u | 200u/μl |
|
M-MLV RT 5X Buffer |
M531A | 1 × 1ml |
