M-MLV Reverse Transcriptase, RNase H Minus

• 5X Reaction Buffer添付：250mM Tris-HCl (pH 8.3 at 25℃), 375mM KCl, 15mM MgCl₂, 50mM DTT
• 70℃, 10分で不活性化します

• 第1鎖cDNA合成
• プライマー伸長反応

1. Roth, M.J. et al. (1985) J. Biol. Chem. 260, 9326–35.
2. Tanese, N. and Goff, S.P. (1988) Proc. Natl. Acad. Sci. USA 85, 1777–81.

Storage Buffer: 20mM Tris-HCl (pH 7.5 at 25°C), 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease.

Unit Definition: One unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 7mM MgCl₂, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [³H]dTTP, 0.025mM oligo(dT), 0.25mM poly(A) and 0.01% NP-40.