Pfu DNA Polymerase
High-Fidelity PCR
- Low error rate thermostable DNA polymerase
- Provided with 10X buffer containing 20mM MgSO4
- Recommended for applications that require high accuracy base insertion
- Supplied at a concentration of 2–3u/μl
Catalog Number:
Size
Catalog Number: M7741
Catalog Number: M7745
Pfu DNA Polymeraseは正確性 (エラ－の少なさ) が求められるPCRに最適な耐熱性DNAポリメラーゼです。本酵素はPyrococcus furiosis DSM3638から単離された分子量92kDa (SDS-PAGE分析) のネイティブ酵素です。耐熱性に優れており、マグネシウム存在下75°Cで高い5'-3'伸長活性を示し、DNAを複製します。また、“プルーフリーディング活性”として知られる3'-5'エキソヌクレアーゼ活性を持ち、耐熱性酵素の中でも最も低いエラー率を示します。この酵素で合成されたPCR断片は平滑末端を形成します。
Pfu DNA Polymerase 10X Reaction Buffer with MgSO4: 200mM Tris-HCl (pH 8.8 at 25°C), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1.0% Triton® X-100, 1mg/ml nuclease-free BSA.
- 高忠実な増幅に最適
- 耐熱性DNAポリメラーゼで最も低レベルのエラー率
- 20mM MgSO4を含む10X反応バッファー添付
PCR、プライマーエクステンションや高い忠実性が要求されるアプリケーションに最適です。
- クローニング
- DNA 発現
- 変異導入分析
さらに知りたい方は、プロトコール＆ガイド(英語) をご覧ください。
【Ｑ＆Ａ】Pfu DNA Polymerase
- Fiala, G. and Stetter, K.O. (1986) Arch. Microbiol. 145, 56.
- Lundberg, K.S. et al. (1991) Gene 108, 1–6.
- Flaman, J.M. et al. (1994) Nucl. Acids Res. 22, 3259–60.
- Cline, J. et al. (1996) Nucl. Acids Res. 24, 3546–51.
- Andre, P. et al. (1997) Genome Res. 7, 843–52.
Important Publications
With a low error rate, Pfu DNA Polymerase is optimally used for high-fidelity PCR and synthesis. Our product has been used in a wide variety of applications—such as gene cloning, gene expression or mutation analysis—across several fields of study.
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Publication |
Summary |
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Mesalam, A.A. et al. (2018) Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1. Virology 514, 30–41. |
The authors used Pfu DNA Polymerase to accurately amplify and subsequently clone the envelope glycoprotein region in the hepatitis C virus (HCV) for development of a human monoclonal antibody. |
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Pasello, M. et al. (2018) Construction of Human Naïve Antibody Gene Libraries. Methods in Molecular Biology 1827. |
The authors used Pfu DNA Polymerase to construct human naïve antibody gene libraries with high affinity and variability. |
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Harvey, J.A. et al. (2018) Ant-like Traits in Wingless Parasitoids Repel Attack from Wolf Spiders. Journal of Chemical Ecology 44(10), 894–904. |
The authors used Pfu DNA Polymerase to amplify the COI gene in wingless parasitoids and subsequently clone and perform Sanger sequencing to reconstruct a partial phylogenetic tree of the organism. |
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Dubos, M.P. et al. (2018) Characterization of a tachykinin signaling system in the bivalve mollusc Crassostrea gigas. General and Comparative Endocrinology 266, 110–118. |
The authors used Pfu DNA Polymerase to amplify the coding sequence of the Cragi-TKR gene from a cDNA library, subsequently cloned into a eukaryotic expression vector, transiently transfected using FuGENE® HD, and calcium responses were measured. |
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Tanabe, E.L.L. et al. (2018) Report of East-Central South African Chikungunya virus genotype during the 2016 outbreak in the Alagoas State, Brazil. Revista do Instituto de Medicina Tropical de São Paulo 60. |
The authors first performed reverse transcription on Chikungunya viral RNA using ImProm-II™ Reverse Transcription System to generate cDNA that was then used as a template to amplify the E1 region using Pfu DNA Polymerase. The PCR amplicon was subsequently Sanger sequenced. |
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
Pfu DNA Polymerase |
M774A | 1 × 100u |
Pfu 10X Reaction Buffer w/20mM MgSO4 |
M776A | 1 × 1.2ml |
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
Pfu DNA Polymerase |
M774B | 1 × 500u |
Pfu 10X Reaction Buffer w/20mM MgSO4 |
M776A | 3 × 1.2ml |
