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Multiplexed Detection of HiBiT-Tagged Proteins and Firefly Luciferase in the Same Sample

  • Rapidly identify specific and non-specific effects of compounds
  • Compatible with batch processing and automation
  • Separate and bicistronic systems for co-expression of HiBiT and Fluc available

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Catalog Number: CS1956A08

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Catalog Number: CS1956B08

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Catalog Number: CS1956B09

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Overview
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Protein Quantitation & Detection Hero Art Floating

同一サンプルからのホタルルシフェラーゼとHiBiTタグ付きタンパク質の連続測定

Nano-Glo® HiBiT Dual-Luciferase® Reporter System(HiBiT NanoDLR™)は、1つのサンプルからホタルルシフェラーゼ(Fluc)とHiBiTタグ付きタンパク質の発光を連続測定できる「添加→測定→添加→測定」形式のアッセイシステムです。Flucは構成的に発現するコントロールとして使用され、HiBiTタグ付き標的タンパク質の特異的な変動ではなく、細胞生存率やタンパク質発現に対する化合物の非特異的な影響を評価するための指標となります。これにより、タンパク質調節スクリーニングや関連研究におけるデータの解釈をサポートします。

11アミノ酸からなるHiBiTペプチドタグは、CRISPR/Cas9ゲノム編集とFlucの異所性発現を組み合わせることで、目的のタンパク質に付加することができます。あるいは、バイシストロニック型HiBiT導入ベクターを使用することで、HiBiT融合タンパク質と構成的に発現するFlucの両方を同一mRNA上で発現させることも可能です。

HiBiT NanoDLR™ のワークフロー

18324ma - HiBiT NanoDLR™ workflow

ホタルルシフェラーゼ(Fluc)は、LgBiTを含むONE-Glo™ EX Reagentを添加することで最初に検出される(1)。その後、NanoDLR™ Stop & Glo® Reagentを細胞に添加することで、Flucシグナルを消光すると同時に、NanoBiT®(HiBiT + LgBiT)シグナルが検出される(2)。

Flucシグナルでタンパク質レベルへの特異的/非特異的影響を識別

18325ma-b - Selective HiBiT-BRD4 degradation with dBET1
18325ma-c - General cellular toxicity with staurosporine
18325ma-a - Selective HiBiT-BRD4 degradation with MZ1

2種のPROTACによるHiBiT-BRD4の選択的分解と一般的な細胞毒性の比較 HEK293細胞に、弱いTKプロモーター下でHiBiT-BRD4-IRES-luc2カセットを発現するコンストラクトを一過性導入した。細胞には、MZ1(パネルA)、dBET1(パネルB)、またはstaurosporine(パネルC)を段階希釈して処理し、その後、溶解型HiBiT NanoDLR™ Assayを用いてFluc(青)およびHiBiT(赤)シグナルを測定した。MZ1およびdBET1は、BRD4をそれぞれ異なるE3リガーゼ複合体に選択的に誘導して分解させるのに対し、staurosporineは一般的な細胞毒性を引き起こし、HiBiT-BRD4およびホタルルシフェラーゼ(Fluc)の両方の減少を招いた。

7桁のダイナミックレンジで定量可能

18327ma - Background-subtracted RLU from HiBiT in NanoDLR Stop & Glo Reagent
18326ma - Background-subtracted RLU from firefly luciferase in ONE-Glo EX/LgBiT reagent

発現レベルにかかわらずタグ付きタンパク質を正確に定量可能な広範な直線ダイナミックレンジ HiBiT NanoDLR™ Assay は、内在性レベルで発現する低発現タンパク質であっても定量可能である。

バイシストロニックベクターでHiBiTとFlucを同一mRNA上に発現

18328ma - Create N- or C-terminal HiBiT fusion vectors and express from different promoter strengths (CMV, PGK or TK)

Flucを構成的に発現する多様なバイシストロニックHiBiT導入ベクター:N末端/C末端融合およびCMV・PGK・TK各プロモーターに対応 すべてのベクターは、ブラスチジン選択マーカーを用いた安定発現細胞株の作製に対応している。

特許および免責事項

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Nano-Glo® Luciferase Assay Limited Use Label License


BY USE OF THIS MATERIAL, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.


Researcher may use this material for research use only; no commercial use is allowed. Commercial use means any and all uses of this material by a party in exchange for consideration, including, but not limited to,

  • (1) use in further product manufacture;
  • (2) use in provision of services, information or data; and
  • (3) resale of the material, whether or not such material is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the material. No other use or transfer of this material is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researcher must:

  • (a) use NanoBRET™-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material; or
  • (b) contact Promega to obtain a license for use of the material for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

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Nano-Glo® Luciferase Assay Limited Use Label License


BY USE OF THIS MATERIAL, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.


Researcher may use this material for research use only; no commercial use is allowed. Commercial use means any and all uses of this material by a party in exchange for consideration, including, but not limited to,

  • (1) use in further product manufacture;
  • (2) use in provision of services, information or data; and
  • (3) resale of the material, whether or not such material is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the material. No other use or transfer of this material is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researcher must:

  • (a) use NanoBRET™-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material; or
  • (b) contact Promega to obtain a license for use of the material for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

分析証明書

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Promega DNA Vector Limited Use Label License (LULL)


BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.


  • 1. Usage Restrictions:
    • 1.1. Recipient may use this material for research use only; no commercial use of this material is allowed without the written consent of Promega Corporation.
    • 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including but not limited to product manufacture or resale of the material for any use and sale of derivatives for any use.
    • 1.3. If recipient is a contract research organization (“CRO”), recipient may transfer or sell data or information generated using the material or derivatives to CRO’s customers who request such data or information, provided that the material or derivatives cannot be transferred from CRO to CRO’s customers.
  • 2. Modification Restrictions:
    • 2.1. Recipient shall have no right to modify or otherwise create variations of the nucleotide sequence of any proprietary gene including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes included in fusion vectors.
    • 2.2. Recipient shall have the right to make derivatives of cloning vectors by incorporating exogenous sequences provided the resulting fusion gene has <4 nucleotides deleted from the affected terminus of the proprietary genes.
  • 3. For determinations of luminescence activity of this material and its derivatives, recipient must either:
    • 3.1. Use Promega-branded luminescent assay reagents (LARs); or
    • 3.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 4. For determinations of NanoLuc® or NanoBiT® luminescence activity of this material and its derivatives, recipient must either:
    • 4.1. Use Nano-Glo®-branded LARs; or
    • 4.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 5. For determinations of GloSensor™ activity of this material and its derivatives, recipient must either:
    • 5.1. Use GloSensor™ cAMP Reagent for in vitro and in-cell applications, and VivoGlo™ Luciferin, In Vivo Grade, for animal applications for all determinations of luminescence activity of this product and derivatives); or
    • 5.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 6. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
    • 6.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
    • 6.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
    • 6.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded auto-fluorescent protein.
  • 7. For uses of HaloTag® Technology in this material, recipient must either:
    • 7.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
    • 7.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
  • 8. Transfer of Materials:
    • 8.1. Unmodified material or progeny of such material cannot be transferred.
    • 8.2. Derivatives of cloning vectors can be transferred to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license.
    • 8.3. If recipient is not a CRO, user may transfer this material to a CRO in order to have CRO use the material solely on behalf of recipient to provide recipient data or information, and CRO may transfer or sell such data or information to recipient, provided that recipient provides CRO with a copy of this label license and CRO agrees to comply with this label license.
    • 8.4. Notwithstanding any other provision in this label license, the recipient can transfer the material (original or derivative of a cloning vector) to a third party for the sole purpose of amplification of the vector, provided that at the time of transfer a copy of this label license is given to the third party and the third party agrees to be bound by the terms of this label license. The third party may not use any of the material (original or amplified) for its own purposes or further transfer the material to any other party.
  • 9. Other Uses:
    • 9.1. For any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, contact Promega for supply and licensing information.
  • 10. Disclaimer and Governing Law:
    • 10.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

分析証明書

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保存条件

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パテントおよび免責条項

Promega DNA Vector Limited Use Label License (LULL)


BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.


  • 1. Usage Restrictions:
    • 1.1. Recipient may use this material for research use only; no commercial use of this material is allowed without the written consent of Promega Corporation.
    • 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including but not limited to product manufacture or resale of the material for any use and sale of derivatives for any use.
    • 1.3. If recipient is a contract research organization (“CRO”), recipient may transfer or sell data or information generated using the material or derivatives to CRO’s customers who request such data or information, provided that the material or derivatives cannot be transferred from CRO to CRO’s customers.
  • 2. Modification Restrictions:
    • 2.1. Recipient shall have no right to modify or otherwise create variations of the nucleotide sequence of any proprietary gene including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes included in fusion vectors.
    • 2.2. Recipient shall have the right to make derivatives of cloning vectors by incorporating exogenous sequences provided the resulting fusion gene has <4 nucleotides deleted from the affected terminus of the proprietary genes.
  • 3. For determinations of luminescence activity of this material and its derivatives, recipient must either:
    • 3.1. Use Promega-branded luminescent assay reagents (LARs); or
    • 3.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 4. For determinations of NanoLuc® or NanoBiT® luminescence activity of this material and its derivatives, recipient must either:
    • 4.1. Use Nano-Glo®-branded LARs; or
    • 4.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 5. For determinations of GloSensor™ activity of this material and its derivatives, recipient must either:
    • 5.1. Use GloSensor™ cAMP Reagent for in vitro and in-cell applications, and VivoGlo™ Luciferin, In Vivo Grade, for animal applications for all determinations of luminescence activity of this product and derivatives); or
    • 5.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 6. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
    • 6.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
    • 6.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
    • 6.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded auto-fluorescent protein.
  • 7. For uses of HaloTag® Technology in this material, recipient must either:
    • 7.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
    • 7.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
  • 8. Transfer of Materials:
    • 8.1. Unmodified material or progeny of such material cannot be transferred.
    • 8.2. Derivatives of cloning vectors can be transferred to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license.
    • 8.3. If recipient is not a CRO, user may transfer this material to a CRO in order to have CRO use the material solely on behalf of recipient to provide recipient data or information, and CRO may transfer or sell such data or information to recipient, provided that recipient provides CRO with a copy of this label license and CRO agrees to comply with this label license.
    • 8.4. Notwithstanding any other provision in this label license, the recipient can transfer the material (original or derivative of a cloning vector) to a third party for the sole purpose of amplification of the vector, provided that at the time of transfer a copy of this label license is given to the third party and the third party agrees to be bound by the terms of this label license. The third party may not use any of the material (original or amplified) for its own purposes or further transfer the material to any other party.
  • 9. Other Uses:
    • 9.1. For any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, contact Promega for supply and licensing information.
  • 10. Disclaimer and Governing Law:
    • 10.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

分析証明書

Search by lot number

保存条件

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パテントおよび免責条項

Promega DNA Vector Limited Use Label License (LULL)


BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.


  • 1. Usage Restrictions:
    • 1.1. Recipient may use this material for research use only; no commercial use of this material is allowed without the written consent of Promega Corporation.
    • 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including but not limited to product manufacture or resale of the material for any use and sale of derivatives for any use.
    • 1.3. If recipient is a contract research organization (“CRO”), recipient may transfer or sell data or information generated using the material or derivatives to CRO’s customers who request such data or information, provided that the material or derivatives cannot be transferred from CRO to CRO’s customers.
  • 2. Modification Restrictions:
    • 2.1. Recipient shall have no right to modify or otherwise create variations of the nucleotide sequence of any proprietary gene including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes included in fusion vectors.
    • 2.2. Recipient shall have the right to make derivatives of cloning vectors by incorporating exogenous sequences provided the resulting fusion gene has <4 nucleotides deleted from the affected terminus of the proprietary genes.
  • 3. For determinations of luminescence activity of this material and its derivatives, recipient must either:
    • 3.1. Use Promega-branded luminescent assay reagents (LARs); or
    • 3.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 4. For determinations of NanoLuc® or NanoBiT® luminescence activity of this material and its derivatives, recipient must either:
    • 4.1. Use Nano-Glo®-branded LARs; or
    • 4.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 5. For determinations of GloSensor™ activity of this material and its derivatives, recipient must either:
    • 5.1. Use GloSensor™ cAMP Reagent for in vitro and in-cell applications, and VivoGlo™ Luciferin, In Vivo Grade, for animal applications for all determinations of luminescence activity of this product and derivatives); or
    • 5.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 6. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
    • 6.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
    • 6.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
    • 6.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded auto-fluorescent protein.
  • 7. For uses of HaloTag® Technology in this material, recipient must either:
    • 7.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
    • 7.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
  • 8. Transfer of Materials:
    • 8.1. Unmodified material or progeny of such material cannot be transferred.
    • 8.2. Derivatives of cloning vectors can be transferred to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license.
    • 8.3. If recipient is not a CRO, user may transfer this material to a CRO in order to have CRO use the material solely on behalf of recipient to provide recipient data or information, and CRO may transfer or sell such data or information to recipient, provided that recipient provides CRO with a copy of this label license and CRO agrees to comply with this label license.
    • 8.4. Notwithstanding any other provision in this label license, the recipient can transfer the material (original or derivative of a cloning vector) to a third party for the sole purpose of amplification of the vector, provided that at the time of transfer a copy of this label license is given to the third party and the third party agrees to be bound by the terms of this label license. The third party may not use any of the material (original or amplified) for its own purposes or further transfer the material to any other party.
  • 9. Other Uses:
    • 9.1. For any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, contact Promega for supply and licensing information.
  • 10. Disclaimer and Governing Law:
    • 10.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

分析証明書

Search by lot number

保存条件

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パテントおよび免責条項

Promega DNA Vector Limited Use Label License (LULL)


BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.


  • 1. Usage Restrictions:
    • 1.1. Recipient may use this material for research use only; no commercial use of this material is allowed without the written consent of Promega Corporation.
    • 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including but not limited to product manufacture or resale of the material for any use and sale of derivatives for any use.
    • 1.3. If recipient is a contract research organization (“CRO”), recipient may transfer or sell data or information generated using the material or derivatives to CRO’s customers who request such data or information, provided that the material or derivatives cannot be transferred from CRO to CRO’s customers.
  • 2. Modification Restrictions:
    • 2.1. Recipient shall have no right to modify or otherwise create variations of the nucleotide sequence of any proprietary gene including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes included in fusion vectors.
    • 2.2. Recipient shall have the right to make derivatives of cloning vectors by incorporating exogenous sequences provided the resulting fusion gene has <4 nucleotides deleted from the affected terminus of the proprietary genes.
  • 3. For determinations of luminescence activity of this material and its derivatives, recipient must either:
    • 3.1. Use Promega-branded luminescent assay reagents (LARs); or
    • 3.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 4. For determinations of NanoLuc® or NanoBiT® luminescence activity of this material and its derivatives, recipient must either:
    • 4.1. Use Nano-Glo®-branded LARs; or
    • 4.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 5. For determinations of GloSensor™ activity of this material and its derivatives, recipient must either:
    • 5.1. Use GloSensor™ cAMP Reagent for in vitro and in-cell applications, and VivoGlo™ Luciferin, In Vivo Grade, for animal applications for all determinations of luminescence activity of this product and derivatives); or
    • 5.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 6. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
    • 6.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
    • 6.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
    • 6.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded auto-fluorescent protein.
  • 7. For uses of HaloTag® Technology in this material, recipient must either:
    • 7.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
    • 7.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
  • 8. Transfer of Materials:
    • 8.1. Unmodified material or progeny of such material cannot be transferred.
    • 8.2. Derivatives of cloning vectors can be transferred to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license.
    • 8.3. If recipient is not a CRO, user may transfer this material to a CRO in order to have CRO use the material solely on behalf of recipient to provide recipient data or information, and CRO may transfer or sell such data or information to recipient, provided that recipient provides CRO with a copy of this label license and CRO agrees to comply with this label license.
    • 8.4. Notwithstanding any other provision in this label license, the recipient can transfer the material (original or derivative of a cloning vector) to a third party for the sole purpose of amplification of the vector, provided that at the time of transfer a copy of this label license is given to the third party and the third party agrees to be bound by the terms of this label license. The third party may not use any of the material (original or amplified) for its own purposes or further transfer the material to any other party.
  • 9. Other Uses:
    • 9.1. For any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, contact Promega for supply and licensing information.
  • 10. Disclaimer and Governing Law:
    • 10.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

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Promega DNA Vector Limited Use Label License (LULL)


BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.


  • 1. Usage Restrictions:
    • 1.1. Recipient may use this material for research use only; no commercial use of this material is allowed without the written consent of Promega Corporation.
    • 1.2. “Commercial use” means any and all uses of this material or derivatives by recipient for monetary or other consideration, including but not limited to product manufacture or resale of the material for any use and sale of derivatives for any use.
    • 1.3. If recipient is a contract research organization (“CRO”), recipient may transfer or sell data or information generated using the material or derivatives to CRO’s customers who request such data or information, provided that the material or derivatives cannot be transferred from CRO to CRO’s customers.
  • 2. Modification Restrictions:
    • 2.1. Recipient shall have no right to modify or otherwise create variations of the nucleotide sequence of any proprietary gene including but not limited to luciferase, NanoBiT® technology (e.g., HiBiT), HaloTag® technology or genes included in fusion vectors.
    • 2.2. Recipient shall have the right to make derivatives of cloning vectors by incorporating exogenous sequences provided the resulting fusion gene has <4 nucleotides deleted from the affected terminus of the proprietary genes.
  • 3. For determinations of luminescence activity of this material and its derivatives, recipient must either:
    • 3.1. Use Promega-branded luminescent assay reagents (LARs); or
    • 3.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 4. For determinations of NanoLuc® or NanoBiT® luminescence activity of this material and its derivatives, recipient must either:
    • 4.1. Use Nano-Glo®-branded LARs; or
    • 4.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 5. For determinations of GloSensor™ activity of this material and its derivatives, recipient must either:
    • 5.1. Use GloSensor™ cAMP Reagent for in vitro and in-cell applications, and VivoGlo™ Luciferin, In Vivo Grade, for animal applications for all determinations of luminescence activity of this product and derivatives); or
    • 5.2. Contact Promega to obtain a license for the use of LARs not manufactured by Promega.
  • 6. For uses of this material for energy transfer (such as bioluminescence resonance energy transfer), recipient must:
    • 6.1. Use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity by this material and its derivatives; and
    • 6.2. Use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this material and its derivatives; or
    • 6.3. Contact Promega to obtain a license for the use of the material and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded auto-fluorescent protein.
  • 7. For uses of HaloTag® Technology in this material, recipient must either:
    • 7.1. Use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties; or
    • 7.2. Contact Promega to obtain a license if Promega HaloTag® ligands are not to be used.
  • 8. Transfer of Materials:
    • 8.1. Unmodified material or progeny of such material cannot be transferred.
    • 8.2. Derivatives of cloning vectors can be transferred to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license.
    • 8.3. If recipient is not a CRO, user may transfer this material to a CRO in order to have CRO use the material solely on behalf of recipient to provide recipient data or information, and CRO may transfer or sell such data or information to recipient, provided that recipient provides CRO with a copy of this label license and CRO agrees to comply with this label license.
    • 8.4. Notwithstanding any other provision in this label license, the recipient can transfer the material (original or derivative of a cloning vector) to a third party for the sole purpose of amplification of the vector, provided that at the time of transfer a copy of this label license is given to the third party and the third party agrees to be bound by the terms of this label license. The third party may not use any of the material (original or amplified) for its own purposes or further transfer the material to any other party.
  • 9. Other Uses:
    • 9.1. For any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, contact Promega for supply and licensing information.
  • 10. Disclaimer and Governing Law:
    • 10.1. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

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