pBiT3.1 HiBiT MCS Vectors
Traditional Cloning Vectors for Generating HiBiT-Tagged Proteins
- Add an N- or C-terminal HiBiT tag to your protein of interest
- Generate an N-terminal HiBiT-tagged transmembrane or secreted protein
- Use for expression analysis and protein quantitation using luminescent detection reagents
Vectors
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| N2361 | pBiT3.1-N[CMV/HiBiT/Blast] Vector | View Specifications | 20μg | Please Enquire | |||
| N2371 | pBiT3.1-C[CMV/HiBiT/Blast] Vector | View Specifications | 20μg | Please Enquire | |||
| N2381 | pBiT3.1-secN[CMV/HiBiT/Blast] Vector | View Specifications | 20μg | Please Enquire |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
3種類の pBiT3.1 HiBiT Vector は目的タンパク質の N末端または C末端に 11アミノ酸のHiBiT ペプチドタグを付加するために設計された発現ベクターです。これらのベクターにはインフレームの HiBiT 融合タンパク質発現のためのマルチクローニング領域が含まれます。pBiT3.1 Vector は安定あるいは一過性発現の両方に使用することができ、細菌でのセレクション用にカナマイシン耐性遺伝子、哺乳動物セレクション用のブラストサイジン耐性遺伝子をコードしています。目的タンパク質と HiBiT間のリンカーは使用する制限酵素によって長さが変わります。
pBiT3.1-N [CMV/HiBiT/Blast] Vector は目的遺伝子の N末端に HiBiTタグの配列を付加
pBiT3.1-C [CMV/HiBiT/Blast] Vector は目的遺伝子の C末端に HiBiTタグの配列を付加
備考：インサートにはN末端側に ATGコドンまたは Kozak 配列を含む適切な翻訳開始配列が必要で、ストップコドンは含まれてはいけません。
pBiT3.1-secN [CMV/HiBiT/Blast] Vector は膜貫通タンパク質または分泌タンパク質の N末端に HiBiTタグを付加。本ベクターは細胞表面での発現あるいは分泌タンパク質のために HiBiT タグのN末端側へ IL-6 分泌シグナルペプチドをコードしたベクターです。
備考：インサートには翻訳停止のために3´ 末端にストップコドンが必要です。
各種 Nano-Glo® HiBiT detection system と HiBiTペプチドタグを組合わせることで目的タンパク質を発光検出することができます。これにより抗体を必要としないタンパク質の発光検出が可能になります。
備考：
1. HiBiT付きタンパク質の発現は、HiBiTタグと目的遺伝子との間で適切な読み枠が維持されている必要があります。
2. HiBiTペプチド配列は参照用です。HiBiT タグを合成する権利等については使用条件についてご確認ください。
![14375MA-W; pBiT3.1-N [CMV/HiBiT/Blast] Vector map](/-/media/images/resources/figures/14300-14399/14375ma-w.svg?rev=da0fb050f4544719a30a52e8804cffc0&sc_lang=ja-jp)
pBiT3.1-N [CMV/HiBiT/Blast] Vector sequence text file.
![14376MA-W; pBiT3.1-C [CMV/HiBiT/Blast] Vector map](/-/media/images/resources/figures/14300-14399/14376ma-w.svg?rev=01148837ac24425eb9a3a227143fc629&sc_lang=ja-jp)
pBiT3.1-C [CMV/HiBiT/Blast] Vector sequence text file.
![14377MA-W; pBiT3.1-secN [CMV/HiBiT/Blast] Vector map](/-/media/images/resources/figures/14300-14399/14377ma-w.svg?rev=b9ef167b5103400494411f3fc64d3651&sc_lang=ja-jp)
pBiT3.1-secN [CMV/HiBiT/Blast] Vector sequence text file.
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
pBiT3.1-N[CMV/HiBiT/Blast] Vector |
N236A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
No transfer of this product is allowed. Researchers may use this product in their own research and they may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to recipients and recipients agree to be bound by the terms of this label license.
Researchers shall have no right to modify or otherwise create variations of the nucleotide sequences of the subunits of NanoBiT® Technology, except that researchers may create fused gene sequences at the termini of the subunits of NanoBiT® Technology. No other use of this product or its derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must either:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives, or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must either:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives, or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researcher has no right to modify, derivatize, genetically engineer or otherwise create variations of the NanoBiT® Control Vectors, except that researcher may propagate and store NanoBiT® Control Vectors for use in NanoBiT® assays. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 9,797,890 and 10,648,971; Japanese Pat. Nos. 7270842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
pBiT3.1-C[CMV/HiBiT/Blast] Vector |
N237A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
No transfer of this product is allowed. Researchers may use this product in their own research and they may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to recipients and recipients agree to be bound by the terms of this label license.
Researchers shall have no right to modify or otherwise create variations of the nucleotide sequences of the subunits of NanoBiT® Technology, except that researchers may create fused gene sequences at the termini of the subunits of NanoBiT® Technology. No other use of this product or its derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must either:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives, or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must either:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives, or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researcher has no right to modify, derivatize, genetically engineer or otherwise create variations of the NanoBiT® Control Vectors, except that researcher may propagate and store NanoBiT® Control Vectors for use in NanoBiT® assays. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 9,797,890 and 10,648,971; Japanese Pat. Nos. 7270842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
pBiT3.1-secN[CMV/HiBiT/Blast] Vector |
N238A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
No transfer of this product is allowed. Researchers may use this product in their own research and they may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to recipients and recipients agree to be bound by the terms of this label license.
Researchers shall have no right to modify or otherwise create variations of the nucleotide sequences of the subunits of NanoBiT® Technology, except that researchers may create fused gene sequences at the termini of the subunits of NanoBiT® Technology. No other use of this product or its derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must either:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives, or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must either:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives, or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researcher has no right to modify, derivatize, genetically engineer or otherwise create variations of the NanoBiT® Control Vectors, except that researcher may propagate and store NanoBiT® Control Vectors for use in NanoBiT® assays. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 9,797,890 and 10,648,971; Japanese Pat. Nos. 7270842 and 7532562; and other patents and patents pending.
Resources
No related resources available
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