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TnT® T7 Quick for PCR DNA

Part Numbers: L5540

L5540_TnT T7 Quick for PCR DNA_3
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PCR 産物からの in vitro 転写 / 翻訳 カップリングシステム（ウサギ網状赤血球ライセート）

  • PCR 産物を精製せずにそのままタンパク質発現に利用可能
  • 直鎖状の鋳型からの標準的な翻訳に比べ最大 5 倍のタンパク質を合成
  • Recombinant RNasin® Ribonuclease Inhibitor も含有

Size

選択製品のカタログ番号: L5540

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This product is discontinued
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
TnT® T7 Quick for PCR DNA
40 reactions
Your price:

Protein from PCR Templates, No Clean Up Required

TNT® T7 Quick for PCR DNAは迅速、簡便な転写/翻訳カップルシステムであり、PCR断片を鋳型としてタンパク質発現を行うために最適化されています。多くのPCR産物で、他の市販のキットよりも最大5倍のタンパク質が得られます。このシステムではPCR後の増幅産物を精製や洗浄をせずに、PCR反応液をそのままTNT®反応に使用することができます。市販のキットや従来の方法で精製したPCR断片も使用可能です。


  • 簡略化：PCR反応後そのままTNT®反応へ移行（精製不要）
  • 高収量：標準的なTNT®システムの最大5倍のタンパク質が得られます。
  • 迅速：1回の翻訳反応に1回の分注のみ
  • 完全：Qick Master MixにはRNasin® Ribonuclease Inhibitorが添加済み
  • 価値：マスターミックス形式を採用しているため、最後まで試薬を有効活用
  • 確実性：TNT® Systemsは厳密な品質検査をしているため、高レベルの転写/翻訳が可能です。

さらに知りたい方は、Protein Expressionをご覧ください。


【Ｑ＆Ａ】TNT.

【ガイド】RNAアプリケーションガイド


  1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

プロトコール

Complete Protocol

Quick Protocols

製品仕様

選択製品の構成品内容

Item Part # Size Concentration Available Separately

Methionine

 L118A 1 × 50μl 1mM

TnT® T7 PCR Quick Master Mix

 L554A 8 × 200μl

Nuclease-Free Water

 P119A 1 × 1,250μl View Product

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

AA

技術資料

サイテーション

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関連製品

RNasin® Ribonuclease Inhibitor

真核生物由来RNaseの広範な阻害において最も信頼されている製品

N2111, N2115, N2511, N2515, N2611, N2615

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