G9801_HaloTag

BRET-Based Assays to Detect Protein:Protein Interactions in Live Cells

  • Ligand for HaloTag® fusion protein in NanoBRET® assays
  • Large spectral separation between NanoLuc® emission (460nm) and NanoBRET® fluorophore emission (618nm)

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Catalog Number: G9801

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NanoBRET™ assayはNanoLuc® ルシフェラーゼをエネルギー転移ドナー、HaloTag® NanoBRET™ 618 fluorescent Ligand で標識されたHaloTag® タンパク質をエネルギー転移アクセプターとして利用するBRET(生物発光共鳴エネルギー転移)ベースのアッセイで、生細胞内で2つのタンパク質の相互作用を測定することができます。

Explore NanoBRET® Nano-Glo® Detection Systems, the complete NanoBRET® PPI solution with this ligand and substrate included.

Protocols

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Specifications

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選択製品の構成品内容

Item Part # Size Available Separately

HaloTag® NanoBRET® 618 Ligand

G980A 1 × 20μl View Product

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

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パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

U.S. Pat. Nos. 9,056,885, 10,139,400 and 10,215,751; European Pat. No. 2782916; and Japanese Pat. Nos. 6522339, 6574821 and 7227741.