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NanoBRET® Nano-Glo® Detection Systems

Part Numbers: N1661, N1662, N1663, N2583, N2584, N2585

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NanoBRET™ Protein Interaction Assay 専用検の検出試薬セット

  • 生細胞内で完全長のタンパク質間相互作用を測定
  • 単一時点または2時間未満の分析には Standard 試薬を、長時間の生細胞測定には Kinetic 試薬を使用
  • 従来の BRET アッセイよりも、高いシグナルと低いバックグラウンドを実現

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選択製品のカタログ番号: N1661

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NanoBRET® Nano-Glo® Detection Systems
Standard/200 assays
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NanoBRET™ タンパク質相互作用アッセイ用の検出試薬

NanoBRET™ assayはNanoLuc® ルシフェラーゼをエネルギー転移ドナー、HaloTag® NanoBRET™ 618 fluorescent Ligand で標識されたHaloTag® タンパク質をエネルギー転移アクセプターとして利用するBRET(生物発光共鳴エネルギー転移)ベースのアッセイで、生細胞内で2つのタンパク質の相互作用を測定することができます。

NanoBRET™ Nano-Glo® Detection System には、ドナーシグナルを生成するためのNanoLuc® ルシフェラーゼの基質であるNanoBRET™ Nano-Glo® Substrateと 蛍光エネルギーアクセプターのためのHaloTag® NanoBRET™ 618 Ligandが含まれます 。HaloTag® NanoBRET™ 618 Ligand は細胞播種時に直接添加し、NanoBRET™ Nano-Glo® Substrateはドナーとアクセプターの発光/蛍光測定直前にサンプルに添加します。

タンパク質間相互作用のためのNanoBRET™ assayについてはNanoBRET™ Protein Interaction Assayをご覧ください。NanoBRET™ Nano-Glo® Detection System は構築済みあるいはカスタム化 NanoBRET™ PPI Assay にも適合します。

Note: Cat.# N1661 is sufficient for 200 assays in 96-well plates or 500 assays in 384-well plates. 
 Cat.# N1662 is sufficient for 1,000 assays in 96-well plates or 2,500 assays in 384-well plates. 
 Cat.# N1663 is sufficient for 10,000 assays in 96-well plates or 25,000 assays in 384-well plates.

Q&A NanoBRET 
【ライセンス】NanoBRET

NanoBRET™ PPI Assay の測定原理

Energy Transfer from NanoLuc to HaloTag NanoBRET Ligand 12749TA-W-a
Depiction of energy transfer from a NanoLuc®-Protein A fusion (energy donor) to a fluorescently labeled HaloTag®-Protein B fusion (energy acceptor) upon interaction of Protein A and Protein B. 
Donor and Acceptor Signal Wavelengths for NanoBRET
Spectral separation of the NanoLuc® emission (460nm) and the fluorescent HaloTag® NanoBRET® 618 Ligand emission (618nm), and calculation of the NanoBRET® ratio.

シンプルなプロトコールで時間も短縮

12750MA-W

タンパク質相互作用アッセイデータの例

The NanoBRET® Nano-Glo® Detection System provides live-cell analysis for single-time-point or short (<2 hours) kinetic assays.

12751MA-W-a

Activation of the EGFR/GRB2 interaction by EGF induction.

12751MA-W-b

Inhibition of the BRD4/Histone H3.3 interaction by JQ1.

The NanoBRET® Nano-Glo® Kinetic Detection System provides increased signal stability of the bioluminescent donor, enabling interaction kinetics to be assayed for multiple hours.

BRD4 ubiquitination kinetics

BRD4 ubiquitination kinetics following treatment with various PROTAC compounds.

Live-cell kinetic measurement of NanoLuc-KRAS 2B G12D / BRAF RBD-HaloTag interaction.

Dissociation kinetics of KRAS 2B G12D/BRAF RBD interaction following treatment with KRAS inhibitor.

製品仕様

You are viewing: N1661 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

HaloTag® NanoBRET® 618 Ligand

G980A 1 × 20μl View Product

NanoBRET® Nano-Glo® Substrate

N157A 1 × 50μl View Product

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

製品仕様

You are viewing: N1662 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

HaloTag® NanoBRET® 618 Ligand

G980A 5 × 20μl View Product

NanoBRET® Nano-Glo® Substrate

N157A 5 × 50μl View Product

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

製品仕様

You are viewing: N1663 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration Available Separately

HaloTag® NanoBRET® 618 Ligand

G980B 1 × 1ml 0.1mM View Product

NanoBRET® Nano-Glo® Substrate

N157B 2 × 1.25ml View Product

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

製品仕様

You are viewing: N2583 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

HaloTag® NanoBRET® 618 Ligand

G980A 1 × 20μl View Product

Nano-Glo® Vivazine® Substrate, 100X

N258A 2 × 0.1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. No. 10,836,767; European Pat. No. 3649130 and Japanese Pat. No. 7204728.

製品仕様

You are viewing: N2584 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

HaloTag® NanoBRET® 618 Ligand

G980A 5 × 20μl View Product

Nano-Glo® Vivazine® Substrate, 100X

N258B 1 × 1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. No. 10,836,767; European Pat. No. 3649130 and Japanese Pat. No. 7204728.

製品仕様

You are viewing: N2585 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration Available Separately

HaloTag® NanoBRET® 618 Ligand

G980B 1 × 1ml 0.1mM View Product

Nano-Glo® Vivazine® Substrate, 100X

N258B 10 × 1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. No. 10,836,767; European Pat. No. 3649130 and Japanese Pat. No. 7204728.

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