NanoBRET® PPI Starter Systems
BRET-Based Assays to Detect Protein:Protein Interactions in Live Cells
- Study induction and inhibition of protein interactions using full-length proteins expressed at physiologically relevant levels
- Starter Systems provide fusion vectors, positive controls and detection reagents for building PPI assays
- Live-cell detection options for endpoint and kinetic analysis
Catalog Number:
Choose a kit
Catalog Number: N1811
Catalog Number: N1821
自然な細胞環境でのタンパク質間相互作用を解析する高感度かつスケーラブルなアッセイ
NanoBRET®を用いたタンパク質間相互作用（PPI）アッセイは、NanoLuc®ルシフェラーゼをBRETのエネルギードナーとして使用し、NanoBRET® 618蛍光色素で標識されたHaloTag®タンパク質をエネルギーアクセプターとして用いることで、特定のタンパク質ペア間の相互作用を測定します。
NanoBRET® PPI Starter Systemには、NanoLuc®ルシフェラーゼおよびHaloTag®タンパク質を目的のタンパク質に融合させるための発現ベクター、ポジティブコントロールペア（p53およびMDM2）、NanoLuc®ルシフェラーゼによるドナーシグナル生成に必要な基質と、蛍光アクセプターとして機能するHaloTag® NanoBRET® 618リガンドを含むNanoBRET® Nano-Glo® Detection Systemが含まれます。
- フルレングスタンパク質または断片を用いた、ライブセルでのタンパク質間相互作用のリアルタイム検出
- 相互作用の誘導と阻害の両方を評価可能な可逆的アッセイ技術
- 96ウェルまたは384ウェルフォーマットに対応し、低い変動性と高い再現性を実現
NanoBRET®初期検討フローとベクター構築サービスについて
【Q&A】 NanoBRET®
【ライセンス】プロメガライセンスプログラム
最適化されたPPIアッセイのための優れたシグナル分離
NanoBRET®テクノロジーは、細胞本来の環境において、タンパク質間相互作用を高感度かつ再現性高く検出することを可能にする技術です。低発現レベルでフルレングスタンパク質を発現させることで、細胞の生理状態をより忠実に反映したPPIのモニタリングやスクリーニング研究が行えます。
NanoBRET®では、青方偏移した明るいドナーシグナルと赤方偏移したアクセプターの組み合わせにより、スペクトルオーバーラップが最適化され、従来のBRETアッセイと比較してシグナル強度の向上とバックグラウンドの低減が実現しています。
NanoBRET®テクノロジーの性能や実際のデータについては、以下の論文で詳しくご覧いただけます： NanoBRET—A Novel BRET Platform for the Analysis of Protein–Protein Interactions, Machleidt et al., (2015) ACS Chem. Biol. 10(8), 1797–1804.
スターターセットおよび”構築済み” または ""カスタム" アッセイも利用可能
ご自身の研究ニーズに合ったフォーマットで、NanoBRET® PPIアッセイを始めてみませんか。NanoBRET®スターターシステムを使って独自のアッセイを設計することも、あらかじめ最適化されたアッセイキットを選択することも可能です。既製アッセイには、最新のRAS経路相互作用アッセイを含む、人気の研究ターゲットが多数ラインアップされています。また、ご希望の研究内容に特化したカスタムアッセイの設計についても、お気軽にご相談いただけます。
NanoBRET® スターターシステムのベクターとアッセイデザイン
NanoBRET® PPI MCS Starter System：複数のクローニング部位（MCS）への従来のクローニング手法を用いて、目的のタンパク質にNanoLuc®ルシフェラーゼおよびHaloTag®タンパク質をN末端またはC末端に融合させることができます。
NanoBRET® PPI Flexi® Starter System：目的のタンパク質にNanoLuc®ルシフェラーゼおよびHaloTag®タンパク質をN末端またはC末端に融合させることができます。このクローニングシステムは、2つのレアカッター制限酵素（SgfI および PmeI）を用いた方向性のあるクローニング法で、Flexi® Vector間でタンパク質コード領域を迅速、高精度で効率よく移し換えることができ、シークエンシングによる確認も不要です。NanoLuc® およびHaloTag® 融合タンパク質発現用のFlexi® ORFクローン（Flexi® クローニング対応）ならすぐにPPI実験を開始することができます。
検討用セット作製受託サービス：細胞内発現を利用したPPI 実験では初期検討に8種類のコンストラクトを作成する必要があります（右図参照）。面倒なコンストラクト作成をまとめて受託いたします。
検出装置に求められる機能
NanoBRET® PPI Assay を実施するためには GloMax® Discover のような適切なフィルターを装着でき、2重フィルタリング発光を連続測定できる検出装置が必要です。
ドナーシグナル (例, 蛍光波長 450nm/BP 80nm) を測定するための中心波長が約460nmのバンドパス (BP) フィルターおよびアクセプターシグナル (例, 蛍光波長 610nm/LP) を測定するための約 600–610nm からスタートするロングパス (LP) フィルターが理想的なフィルターセットアップです。この範囲外のフィルターを使用するとデータの質が低下する恐れがあります。
RentaMAX プログラムは GloMax® Discover などの検出装置をレンタルするプロメガクラブ会員へのサービスです。詳細はこちらをご覧ください。
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
|
pHTC HaloTag® CMV-neo Vector |
G7711 | 1 × 20μg | |
|
pHTN HaloTag® CMV-neo Vector |
G7721 | 1 × 20μg | |
pNLF1-N [CMV/Hygro] Vector |
N1351 | 1 × 20μg | 1μg/μl |
pNLF1-C [CMV/Hygro] Vector |
N1361 | 1 × 20μg | 1μg/μl |
NanoBRET® PPI Control Pair (p53, MDM2) |
N1641 | 1 × 2 × 20μg | |
NanoBRET® Nano-Glo® Detection System |
N1661 | 1 × 200 assays |
SDS
Search for SDS分析証明書
使用制限
For Research Use Only. Not for Use in Diagnostic Procedures.保存条件
Please see the Protocol for detailed storage conditions.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene or the HaloTag® gene except that researcher may create fused gene sequences provided that the coding sequence of the resulting luciferase gene or HaloTag® gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact gene sequence. No other use of this product or derivatives is authorized without the prior express written consent of Promega.
In addition, researcher must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives, or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researcher must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives, or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researcher may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researchers may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researcher may (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use of this product or derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,420,367, 8,748,148, 9,593,316 and 9,873,866; European Pat. Nos. 2374875, 2492342 and 2502990; and Japanese Pat. Nos. 5680302 and 5840117.
U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.
U.S. Pat. No. 10,024,862; Japanese Pat. No. 7676487; and other patents and patents pending.
U.S. Pat. Nos. 9,056,885, 10,139,400 and 10,215,751; European Pat. No. 2782916; and Japanese Pat. Nos. 6522339, 6574821 and 7227741.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
|
pFN21A HaloTag® CMV Flexi® Vector |
G2821 | 1 × 20μg | |
|
pFC14K HaloTag® CMV Flexi® Vector |
G9661 | 1 × 20μg | |
pFN31K Nluc CMV-neo Flexi® Vector |
N1321 | 1 × 20μg | 0.1μg/μl |
pFC32K Nluc CMV-neo Flexi® Vector |
N1341 | 1 × 20μg | 0.1μg/μl |
NanoBRET® PPI Control Pair (p53, MDM2) |
N1641 | 1 × 2 × 20μg | |
NanoBRET® Nano-Glo® Detection System |
N1661 | 1 × 200 assays |
SDS
Search for SDS分析証明書
使用制限
For Research Use Only. Not for Use in Diagnostic Procedures.保存条件
Please see the Protocol for detailed storage conditions.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene or the HaloTag® gene except that researcher may create fused gene sequences provided that the coding sequence of the resulting luciferase gene or HaloTag® gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact gene sequence. No other use of this product or derivatives is authorized without the prior express written consent of Promega.
In addition, researcher must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives, or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researcher must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives, or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researcher may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researchers may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researcher may (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use of this product or derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 8,420,367, 8,748,148, 9,593,316 and 9,873,866; European Pat. Nos. 2374875, 2492342 and 2502990; and Japanese Pat. Nos. 5680302 and 5840117.
U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.
U.S. Pat. No. 10,024,862; Japanese Pat. No. 7676487; and other patents and patents pending.
U.S. Pat. Nos. 9,056,885, 10,139,400 and 10,215,751; European Pat. No. 2782916; and Japanese Pat. Nos. 6522339, 6574821 and 7227741.
Resources
No related resources available
Related Products
類似製品
NanoBRET® VHL and CRBN Ternary Complex Starter Kits
生細胞における VHL または CRBNと標的タンパク質との三重複合体形成をBRETにより定量
ND2700, ND2720
NanoBRET® Ubiquitination Starter Kit
特定タンパク質を標的としたユビキチンタイプのすべてを測定する生細胞ベースのユビキチンアッセイ
ND2690
NanoBRET® Proteasomal Recruitment Starter Kit
26S プロテアソームへのリクルートをモニタリングする特定タンパク質を標的とした生細胞アッセイ
ND2730
NanoBRET® BiBRET PPI Starter System
Live-cell PPI detection using bright NanoBRET® chemistry and single-vector convenience.
CS1679C169
関連製品
NanoBRET® Nano-Glo® Detection Systems
NanoBRET™ Protein Interaction Assays専用検出システム
N1661, N1662, N1663, N2583, N2584, N2585
GloMax® Discover System
発光・蛍光・吸光度測定に対応した高性能マイクロプレートリーダー
GM3000
Nano-Glo® In-Gel Detection System
ポリアクリルアミドゲル内のNanoLuc® 融合タンパク質を直接的に発光検出
N3020
CRAF/BRAF BiBRET Control Vector
Positive control for validating and optimizing BiBRET PPI assay performance.
CS1679D24
