HaloTag® Mammalian Protein Purification System

Part Numbers: G6790, G6795

G6790_HaloTag-Mammalian-Protein-Purification
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低発現レベルでも哺乳動物培養細胞より高純度の組換えタンパク質を回収

  • HaloLink™ Resin は >7mg/ml の HaloTag® 融合タンパク質を供給結合で捕捉 ( FLAG® の約10倍)
  • ミリグラムレベルのタンパク質回収のためのスケールアップも容易(スケールダウンも)
  • 目的に合わせてタンパク質精製、検出するために3種類のキットを用意

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選択製品のカタログ番号: G6790

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HaloTag® Mammalian Protein Purification System
Protein Purification/1 each
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研究目的に合った哺乳動物細胞プルダウンシステムからお選びいただけます

哺乳動物細胞ライセートからのHaloTag®融合タンパク質の精製に最適化されたキットです。HaloTag®融合タンパク質はHaloLink™ Resinと高い特異性で共有結合を形成します。このHaloLink™ Resinへの共有結合と非特異的な結合の低さにより、哺乳動物培養細胞での発現レベルが低い場合でもリコンビナントタンパク質を非常に高い純度、収率で精製することができます。HaloTag® Mammalian Protein Detection and Purification System(Cat.# G6795, G6799)にはHaloTag®染色用のHaloTag® TMRDirect™ Ligandが付属しており、簡便にHaloTag®融合タンパク質を蛍光検出できるため、発現・精製条件を迅速に最適化することができます。システムに付属するHaloLink™ Resinの結合容量はHaloTag®融合タンパク質 >7mg/ml(FLAG® の約10倍)で、回収率も通常 >75%です。非特異的な結合も非常に少なく(<0.1%)高純度のタンパク質を精製することができます。


  • 多くのタンパク質を精製: HaloLink™ Resinへの共有結合容量>7mg/ml HaloTag®融合タンパク質(FLAG®の10倍以上)。高い回収率(通常>75%)
  • 高純度: 共有結合により、結合タンパク質を損失することなく強い洗浄が可能となるため非常に低い非特異的結合(<0.1%)と高純度タンパク質回収を実現
  • スケール変更が自在: スケールアップ/ダウンが容易で、mgオーダー以上の精製も可能
  • 哺乳動物のタンパク質発現に最適化: HaloTag®プラットフォームならばタンパク質精製からプルダウン、細胞内イメージングを単一のコンストラクトで柔軟に実施可能。

  • 哺乳類細胞培養ライゼートからのHaloTag®融合タンパク質精製可能。

【ライセンス】HaloTagシリーズ

【簡易マニュアル】日本語※日本語マニュアルは英語版を翻訳したものですが、常に更新されるものではありません。最新のマニュアルについては英語版(”Product Resources”タブ→“Protocols”)をご覧ください。

【Q&A】HaloTag.

【アプリケーション】HaloTag® を用いた蛋白質に結合するRNAの網羅的解析(PAR-CLIP法)

Streamlined purification process leads to higher purity and recovery of recombinant proteins from cultured cells.

Streamlined purification process leads to higher purity and recovery of recombinant proteins from cultured cells.

製品仕様

You are viewing: G6790 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration Available Separately

HaloLink™ Resin

G1913 1 × 2.5ml

Protease Inhibitor Cocktail, 50X

G652A 2 × 1ml resuspended View Product

HaloTEV Protease

G660A 1 × 200μl 5u/μl View Product

Spin Column

V995A 1 × 25 each

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

See Protocol for detailed storage recommendations.

 

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

製品仕様

You are viewing: G6795 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration Available Separately

HaloLink™ Resin

G1913 1 × 2.5ml

HaloTag® TMRDirect™ Ligand

G299A 1 × 30μl View Product

Protease Inhibitor Cocktail, 50X

G652A 2 × 1ml resuspended View Product

HaloTEV Protease

G660A 1 × 200μl 5u/μl View Product

Spin Column

V995A 1 × 25 each

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

See Protocol for detailed storage recommendations.

 

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

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