Promoterless Firefly Luciferase Basic Vectors

Part Numbers: E6651, E6661, E6671

E6751_pGL4-20-luc2-Puro-Vector_3
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安定性の異なるレポータータンパク質遺伝子を組込み済のプロモーターレスpGL4ベクター

  • 遺伝子転写調節領域の調査のための推定プロモーターエレメントのクローニング用に設計
  • 3種類の改変ルシフェラーゼ遺伝子(luc2luc2Pluc2CP)から選択可能

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選択製品のカタログ番号: E6651

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Promoterless Firefly Luciferase Basic Vectors
luc2/20µg
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pGL4.10[luc2]、pGL4.11[luc2P]、pGL4.12[luc2CP]ベクターは主に遺伝子の転写を制御する重要な領域を調べるための推定プロモーターエレメントを挿入できるように設計されています。3種類の改変ホタルルシフェラーゼ(luc2、luc2P、luc2CP)遺伝子を組込み済のプロモーターレスベクターから選択可能です。これらのベクターにクローニングされたインサートは、マルチクローニングサイトと、ユニークなSfil制限酵素を用いて容易に移し替え可能です。

 

アプリケーション例

  • 転写制御
  • ウイルス-細胞相互作用
  • 化合物スクリーニング
  • 翻訳語修飾
  • プロモーター解析

 

改変ホタルルシフェラーゼの特性

luc2 遺伝子は曖昧な転写因子結合部位をほとんど除去し、コドンの最適化により哺乳動物での発現量を改善しています。luc2P、 luc2CPおよび RapidResponse™ 遺伝子は、ホタルルシフェラーゼの 細胞内半減期を変えるためにluc2 に分解配列を付加したものです。RapidResponse™ 遺伝子は刺激に対して素早く応答しますがシグナル強度が低下します。 luc2P (半減期 約1時間) 遺伝子は中程度のシグナル強度かつluc2 (半減期 約3時間)よりも迅速に応答し、luc2CP (半減期 約0.4時間)はさらに早く応答する一方でシグナルは最も低い値を示します。

pGL4レポーターベクターについて

pGL4ベクターは哺乳動物での発現量改善のために遺伝子のコドン最適化を行い、非特異なDNA制御エレメントや転写因子結合部位の除去によりバックグラウンドおよび発現アーティファクトのリスクを低減しています。組込まれているホタルルシフェラーゼには不安定化配列の付加により応答時間を改善したオプションがあります。また、プロモーターレスベクターは3種類の選択マーカー(ハイグロマイシン、ネオマイシン、ピューロマイシン)または選択マーカーなしからお選びいただけます。

全てのpGL4 ベクターはこちら

 
【ライセンス】pGL4ベクターシリーズ

4721MA-W-01

pGL4.10[luc2] Vector GenBank® Accession Number AY738222 and vector sequence text file.

4722MA-W-01

pGL4.11[luc2P] Vector GenBank® Accession Number AY738223 and vector sequence text file.

4723MA-W-01

pGL4.12[luc2CP] Vector GenBank® Accession Number AY738224 and vector sequence text file

製品仕様

You are viewing: E6651 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.10[luc2] Vector

E665A 1 × 20μg

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

製品仕様

You are viewing: E6661 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.11[luc2P] Vector

E666A 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

製品仕様

You are viewing: E6671 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.12[luc2CP] Vector

E667A 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

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