Promoterless Firefly Luciferase Vectors with Hygromycin Selection

Part Numbers: E6691, E6701, E6711

E6751_pGL4-20-luc2-Puro-Vector_3
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レポータータンパク質の安定性が選べる、プロモーターレスpGL4ベクター(ハイグロマイシン耐性)

  • 遺伝子の転写調節領域を調べるための推定されるプロモーターエレメントのクローニング用にデザイン
  • 3種類の改変ルシフェラーゼ遺伝子(luc2luc2Pluc2CP)から選択可能
  • ハイグロマイシンで安定発現株の選抜が可能

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選択製品のカタログ番号: E6691

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Promoterless Firefly Luciferase Vectors with Hygromycin Selection
luc2/20µg
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プロモーターの無いホタルルシフェラーゼベクターは主に遺伝子の転写をコントロールする重要な領域を調べるための推定されるプロモーターエレメントのクローニング用にデザインされています。プロモーターの無いベクターには3種類の改変ルシフェラーゼ遺伝子( luc2、luc2P および luc2CP)から選べます。luc2 遺伝子はほとんどの曖昧な転写因子結合サイトを除去し、コドンの最適化を行ったことで哺乳動物での発現が向上しました。luc2P、 luc2CPおよび RapidResponse™ 遺伝子は 細胞内半減期を変えるためにluc2 に分解配列を付加したものです。RapidResponse™ 遺伝子は刺激に対して素早く応答しますがシグナル強度が低下ます。 luc2P (半減期 約1時間) 遺伝子は中程度のシグナル強度でluc2 (半減期 約3時間)よりも迅速に応答し、luc2CP (半減期 約0.4時間)はさらに早く応答しますがシグナルは最も低い値を示します。 プロモーター無しのベクターは選択マーカーが含まれるものと含まれないものから選べます(ハイグロマイシン、ネオマイシン、ピューロマイシン)

全てのpGL4 ベクターはこちら


感度の向上と生物学的関連性の強化:

  • レポーター遺伝子発現向上: 哺乳動物細胞発現のための合成遺伝子のコドンの最適化
  • バックグラウンドとアーティファクトの低減: 曖昧な調節エレメントや転写因子結合サイトを除去
  • 応答性の改善: 不安定化ルシフェラーゼ遺伝子を用いたRapid Response™ 技術を利用可能

その他の利点:

  • 柔軟な検出オプション: Choice of either 合成 luc2 (Photinus pyralis) または hRluc (Renilla reniformis) レポーター遺伝子から選択可能
  • トランジェントからステイブルへの変更が容易: 哺乳動物細胞用選択マーカーを選択可能
  • ベクター間の移動が簡便: 共通のマルチクローニングサイトおよびユニークなSfiIトランスファースキーム

  • 転写調節
  • ウイルス-細胞 相互作用 
  • 化合物スクリーニング 
  • 翻訳後修飾
  • GPCR シグナリング
  • 細胞内シグナリング
  • プロモーター解析

Protocols & Applications Guide もご参照ください


【ライセンス】pGL4ベクターシリーズ

How the Engineered Firefly Luciferase Genes Work

The luc2 gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The luc2P and luc2CP and RapidResponse™ genes are luc2 genes appended with degradation sequences to influence the cellular half-life of firefly luciferase. The RapidResponse™ genes respond more rapidly to stimuli but at the expense of signal intensity. The luc2P (1-hour half-life) gene responds more rapidly than luc2 (3-hour half-life) with moderate signal intensity, and the luc2CP (0.4-hour half-life) responds more quickly with the lowest signal intensity.

About the pGL4 Reporter Vectors

The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Firefly luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.

4862MA-W

pGL4.14[luc2/Hygro] Vector GenBank® Accession Number AY864928 and vector sequence text file.

4863MA-W

pGL4.15[luc2P/Hygro] Vector GenBank® Accession Number AY864929 and vector sequence text file.

4864MA-W

pGL4.16[luc2CP/Hygro] Vector GenBank® Accession Number AY864930 and vector sequence text file.

製品仕様

You are viewing: E6691 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.14[luc2/Hygro] Vector

E669A 1 × 20μg

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

製品仕様

You are viewing: E6701 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.15[luc2P/Hygro] Vector

E670A 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

製品仕様

You are viewing: E6711 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.16[luc2CP/Hygro] Vector

E671A 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

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