Stress Signaling Pathway Analysis Response Element Firefly Luciferase Vectors
Firefly Luciferase Reporter Vectors with Cellular Stress Response Elements
- Offer a range of response elements that are involved in stress signalling pathways
- Each vector included an optimized firefly luciferase gene that codes a reporter with a one-hour half-life
- Hygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line
Catalog Number:
Choose a response element
Size
Catalog Number: E3641
Catalog Number: E3651
Catalog Number: E3661
Catalog Number: E4131
Catalog Number: E3751
Catalog Number: E4001
Catalog Number: E4121
パスウェイレポーターの構築には、それぞれ異なる組換えホタルルシフェラーゼ遺伝子 (luc2, luc2P, luc2CP) を持つ３種類の 最小限プロモーター(minP) ベクターがご利用いただけます。Luc2遺伝子からはほとんどの変則的に働く転写因子結合サイトが除去され、コドンの最適化により哺乳動物での発現が向上しました。luc2P、luc2CP およびRapidResponse™ 遺伝子はluc2遺伝子にルシフェラーゼの細胞内半減期に影響を及ぼす分解配列を付加したものです。RapidResponse™ 遺伝子は刺激に対してより素早く応答しますが、シグナル強度を犠牲にしています。luc2P遺伝子（半減期 1時間）はluc2（半減期 3時間）よりもより早く応答しますが、中程度のシグナル強度を示し、luc2CP（半減期 0.4時間）はさらに迅速に応答しますが、シグナル強度は低く抑えられています。このminPベクターは選択マーカー（ハイグロマイシン）有/無の2タイプがご利用いただけます。研究をスピードアップするために、すでに応答配列を組み込んだpGL4.27ベースのプレデザインされたパスウェイベクターをいくつか揃えています。これらのいくつかは安定細胞株 (GloResponse™ Cell Lines) もご用意しています。
- プレデザインベクター：クロニングする手間やアッセイによる確認が不要
- レポーターの発現増加: 哺乳動物発現のためのコドン最適化
- バックグラウンドとアーティファクトのリスクの低減: 変則的なDNA調節エレメントおよび転写因子結合サイトを除去
- 応答時間を改善: ルシフェラーゼ遺伝子の不安化させてたRapid Response™ テクノロジー
- トランジェント/安定発現でも: 哺乳動物用の選択マーカーを選択可能
- 転写調節
- ウイルス-細胞 相互作用
- 化合物スクリーニング
- 翻訳後修飾
- GPCR シグナリング
- 細胞内シグナリング
- プロモーター分析
Protocols & Applications Guide もご参照ください。
【ライセンス】pGL4ベクターシリーズ
About the pGL4 Reporter Vectors
The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Firefly luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.
Sequence Information
pGL4.37[luc2P/ARE/Hygro] Vector GenBank® Accession Number JQ858521 and vector sequence text file.
pGL4.38[luc2P/p53 RE/Hygro] Vector GenBank® Accession Number JQ858522 and vector sequence text file.
pGL4.39[luc2P/ATF6 RE/Hygro] Vector GenBank® Accession Number JQ858519 and vector sequence text file.
pGL4.40[luc2P/MRE/Hygro] Vector GenBank® Accession Number JQ858515 and vector sequence text file.
pGL4.41[luc2P/HSE/Hygro] Vector GenBank® Accession Number JQ858520 and vector sequence text file.
pGL4.42[luc2P/HRE/Hygro] Vector GenBank® Accession Number JQ858518 and vector sequence text file.
pGL4.43[luc2P/XRE/Hygro] Vector GenBank® Accession Number JQ858513 and vector sequence text file.
| Activator/Pathway | Transcription Factor | Binding Site | Vector Name | Example Data |
|---|---|---|---|---|
|
Oxidative stress
|
Nrf2 |
Antioxidant Response Element (ARE)
|
pGL4.37[luc2P/ARE/Hygro]
|
Representative data for pGL4.37[luc2P/ARE/Hygro] Vector in HEK293 cells upon stimulation with tBHQ or D,L-Sulforaphane. HEK293 cells were transiently transfected with pGL4.37[luc2P/ARE/Hygro] and pGL4.75[hRluc>/CMV] Vectors and assayed in 96-well format after 18 hours stimulation with tBHQ or D,L-Sulforaphane as described. Firefly luciferase luminescence normalized to the Renilla> luciferase control is shown, with error bars indicating the S.E.M. for six replicates. Luminescence was detected after addition of Dual-Glo® reagents, using a GloMax® 96 instrument with a 0.5 second integration time. |
|
DNA damage
|
p53 |
p53 response element (p53 RE)
|
pGL4.38[luc2P/p53 RE/Hygro]
|
|
|
Endoplasmic reticulum stress
|
ATF6
|
Activating Transcription Factor 6 response element (ATF6 ERSE)
|
pGL4.39[luc2P/ATF6 RE/Hygro]
|
|
|
Heavy metal stress
|
MTF1
|
Metal Regulatory Element (MRE)
|
pGL4.40[luc2P/MRE/Hygro]
|
|
|
Heat shock
|
HSF1
|
Heat Shock Element (HSE)
|
pGL4.41[luc2P/HSE/Hygro]
|
|
|
Hypoxia
|
Hif1α
|
Hypoxia Response Element (HRE)
|
pGL4.42[luc2P/HRE/Hygro]
|
|
|
Xenobiotic stress
|
AhR |
Xenobiotic Responsive Element (XRE)
|
pGL4.43[luc2P/XRE/Hygro]
|
|
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
| pGL4.37[luc2P/ARE/Hygro] Vector | E364A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
| pGL4.38[luc2P/p53 RE/Hygro] Vector | E365A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
| pGL4.39[luc2P/ATF6 RE/Hygro] Vector | E366A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
| pGL4.40[luc2P/MRE/Hygro] Vector | E413A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
| pGL4.41[luc2P/HSE/Hygro] Vector | E375A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
| pGL4.42[luc2P/HRE/Hygro] Vector | E400A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
| pGL4.43[luc2P/XRE/Hygro] Vector | E412A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
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pGL4.23[luc2/minP]、pGL4.24[luc2P/minP] 、pGL4.25[luc2CP/minP] ベクターは、最小限プロモーター とホタルルシフェラーゼ遺伝子の上流に応答配列をクローニングするためのベクターです。
E8411, E8421, E8431
最小限プロモーターが駆動するホタルルシフェラーゼベクター（ハイグロマイシン耐性）
pGL4.26[luc2/minP/Hygro]、pGL4.27[luc2P/minP/Hygro]、 pGL4.28[luc2CP/minP/Hygro] ベクターは、最小限プロモーター とホタルルシフェラーゼ遺伝子（安定性有り/なし）の上流に応答エレメントをクローニングするためのベクターです。
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サイトカインシグナル伝達経路解析用(最小限プロモーターを含む) ホタルルシフェラーゼベクター
pGL4.45[luc2P/ISRE/Hygro]、pGL4.47[luc2P/SIE/Hygro]、 pGL4.48[luc2P/SBE/Hygro] 、pGL4.52[luc2P/STAT5RE/Hygro] ベクターは様々なサイトカインシグナル経路の研究に使用されます
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その他シグナル伝達経路解析用 (最小限プロモーターによる駆動) ホタルルシフェラーゼベクター
様々なシグナル伝達経路の調査に使用できるpGL4.44[luc2P/AP1 RE/Hygro]、pGL4.49[luc2P/TCF-LEF RE/Hygro]、pGL4.29[luc2P/CRE/Hygro]、pGL4.30[luc2P/NFAT-RE/Hygro]、pGL4.32[luc2P/NF-κB-RE/Hygro]、pGL4.33[luc2P/SRE/Hygro]、pGL4.34[luc2P/SRF-RE/Hygro] Vector
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関連製品
ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay
単一ウェルでレポーター活性と細胞生存性マーカーの高感度検出
E7110, E7120
Bright-Glo™ Luciferase Assay System
ロボットを用いた連続処理にも使える高感度レポーターアッセイ
E2610, E2620, E2650
Steady-Glo® Luciferase Assay System
マルチウェルプレートでのバッチ処理用、長時間発光の高感度レポーターアッセイ
E2510, E2520, E2550
Dual-Luciferase® Reporter Assay System
単一サンプルでのホタル、ウミシイタケルシフェラーゼ活性の連続測定。インジェクター2本必要。
E1910, E1960, E1980
