IL-15 Bioassay
A Simpler, More Consistent IL-15 Bioassay
- Prequalified according to ICH guidelines
- Mechanism-of-action-based assay
- No primary cell culture required
- Supplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format
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Catalog Number: JA2011
Catalog Number: JA2015
Catalog Number: J2962
安定した結果が得られるシンプルな バイオアッセイ
インターロイキン-15 (IL-15) は単球、マクロファージ、樹状細胞および上皮細胞など様々な細胞種より分泌されます。 IL-15 はナチュラルキラー (NK) 細胞、ナチュラルキラーT (NKT) 細胞、 CD8+メモリー T細胞の活性化や増殖を促進します。IL-2 とは異なり、 IL-15 は CD4+ 制御性 T 細胞 (Treg) には影響を及ぼさず、活性化誘導型細胞死 (AICD) も誘導しません。
IL-15 Bioassay は STAT-5 応答配列を利用して IL-15 による刺激や阻害を測定するためにデザインされた細胞ベース発光アッセイです。IL-15 Bioassay には "thaw-and-use" の簡単フォーマットで使用できる凍結保存細胞が含まれており、細胞を融解した後、細胞を増やすことなくそのままアッセイに使用することができます。 IL-15 Bioassay には 融解して、増殖、バンキングが許容された凍結保存細胞を含む長期使用のための Cell Propagation Model (CPM) フォーマットもご利用いただけます。
アッセイの構成品
The IL-15 Bioassay Cells included are provided in a thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell propagation.
The IL-15 Bioassay is also available in a Cell Propagation Model (CPM) format, as cryopreserved cells, that can be thawed, propagated and banked for long-term use (Cat.# J2962).
ワークフロー
従来法
標準化された試薬による最適な結果
- ”解凍 ＆ アッセイ” だけのフォーマットは従来法に比べ飛躍的に時間と労力を削減し、バラつきも低減
- キットは標準化されたフォーマットとして必要なすべての試薬を供給
- ハイスループットワークフローにも容易に適応
アッセイの原理
Assay Performance Data
Accuracy and Precision are critical to bioassays and the development of biologics. The IL-15 Bioassay is both accurate and precise. Accuracy is a measure of how close results are to a target or reference value. Precision is a measure of repeatability. This assay was developed and prequalified according to ICH guidelines for accuracy and precision, giving it the performance characteristics required for many applications of a bioassay used for antibody drug discovery research and development.
Accuracy: Measure of ability to replicate a reference or "target" value.
IntraLab Precision: Measure of repeatability from multiple assays of the same homogeneous sample under the same operating conditions over a short time (same analyst; same lab; same day).
Intermediate Precision: Measure of repeatability of multiple assays of the same homogeneous sample with different analysts or equipment or on different days.
Linearity: Ability of your assay to provide measured values that are proportional to the concentration of the sample.
The IL-15 Bioassay Shows Precision, Accuracy and Linearity.
| Parameter | Results | |
|---|---|---|
| Accuracy | % Expected Relative Potency | % Recovery |
| 50 | 97.8 | |
| 70 | 101.4 | |
| 140 | 97.9 | |
| 200 | 102.7 | |
| Repeatability (% CV) | 100% (Reference) | 1.47 |
| Intermediate Precision (% CV) | 6.75 | |
| Linearity (r2) | 0.998 | |
| Linearity (y = mx + b) | y = 1.03x–2.79 | |
| A 50–200% theoretical potency series of recombinant human IL-15 was analyzed in triplicate in three independent experiments performed on three days by two analysts (for a total of six independent experiments). Bio-Glo™ Reagent was added and luminescence quantified using the GloMax® Discover System. Data were analyzed and relative potencies calculated after parallelism determination using JMP® software. Data were generated using thaw-and-use cells. | ||
The IL-15 Bioassay response to recombinant IL-15.
The IL-15 Bioassay indicates stability.
Product Kit Formats and Related Products
- IL-15 Bioassay is available in 1X and 5X sizes.
- IL-15 Bioassay is also available in CPM format (Cat.# J2962).
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
IL-15 Bioassay Cells |
J298A | 1 × 0.80ml |
Fetal Bovine Serum |
J121A | 1 × 4ml |
RPMI 1640 Medium |
G708A | 1 × 36ml |
Bio-Glo™ Luciferase Assay Buffer |
G719A | 1 × 10ml |
Bio-Glo™ Luciferase Assay Substrate |
G720A | 1 × 1 vial |
SDS
Search for SDS分析証明書
使用制限
Not For Medical Diagnostic Use.保存条件
Please see the Protocol for detailed storage conditions.
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.
This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
IL-15 Bioassay Cells |
J298A | 5 × 0.80ml |
Fetal Bovine Serum |
J121A | 5 × 4ml |
RPMI 1640 Medium |
G708A | 5 × 36ml |
Bio-Glo™ Luciferase Assay Buffer |
G719A | 5 × 10ml |
Bio-Glo™ Luciferase Assay Substrate |
G720A | 5 × 1 vial |
SDS
Search for SDS分析証明書
使用制限
Not For Medical Diagnostic Use.保存条件
Please see the Protocol for detailed storage conditions.
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.
This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
IL-15 Bioassay Cells (CPM) |
J296A | 2 × 0.75ml |
SDS
Search for SDS分析証明書
使用制限
Not For Medical Diagnostic Use.保存条件
Please see the Protocol for detailed storage conditions.
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.
This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells except that recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
Resources
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関連製品
Bio-Glo™ Luciferase Assay System
レポーターバイオアッセイに理想的な安定性の高いルシフェラーゼ試薬
G7940, G7941
GloMax® Discover System
発光・蛍光・吸光度測定に対応した高性能マイクロプレートリーダー
GM3000
RealTime-Glo™ MT Cell Viability Assay
72 時間まで培養細胞の生存性をモニタリングできる発光アッセイ
G9711, G9712, G9713
CellTiter-Glo® 2.0 Cell Viability Assay
試薬の安定性が向上したCellTiter-Glo®、ATP検出に基づく細胞増殖の定量が可能
G9241, G9242, G9243
