IL-15 Bioassay

Part Numbers: JA2011, JA2015, J2962

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シンプルでより安定した結果が得られる IL-15 バイオアッセイ

  • ICH ガイドラインによる事前承認
  • 作用機序ベースのアッセイ
  • 初代培養細胞は不要

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選択製品のカタログ番号: JA2011

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IL-15 Bioassay
Thaw-and-Use Kits/1X each component
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安定した結果が得られるシンプルな バイオアッセイ

インターロイキン-15 (IL-15) は単球、マクロファージ、樹状細胞および上皮細胞など様々な細胞種より分泌されます。 IL-15 はナチュラルキラー (NK) 細胞、ナチュラルキラーT (NKT) 細胞、 CD8+メモリー T細胞の活性化や増殖を促進します。IL-2 とは異なり、 IL-15 は CD4+ 制御性 T 細胞 (Treg) には影響を及ぼさず、活性化誘導型細胞死 (AICD) も誘導しません。

 IL-15 シグナルにより複数のパスウェイが活性化します。リンパ球では JAK1 および JAK3 チロシンキナーゼがリクルートされ受容体の細胞質ドメインで活性化が起こり JAK/STAT シグナルが開始されます。これらのキナーゼによりリン酸化された STAT3 および 5 が二量体/三量体を形成し、核内に移行して様々なタンパク質の転写を活性化させます。

IL-15 Bioassay は  STAT-5 応答配列を利用して IL-15 による刺激や阻害を測定するためにデザインされた細胞ベース発光アッセイです。IL-15 Bioassay には "thaw-and-use" の簡単フォーマットで使用できる凍結保存細胞が含まれており、細胞を融解した後、細胞を増やすことなくそのままアッセイに使用することができます。 IL-15 Bioassay には  融解して、増殖、バンキングが許容された凍結保存細胞を含む長期使用のための Cell Propagation Model (CPM) フォーマットもご利用いただけます。

アッセイの構成品

The IL-15 Bioassay Cells included are provided in a thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell propagation.

The IL-15 Bioassay is also available in a Cell Propagation Model (CPM) format, as cryopreserved cells, that can be thawed, propagated and banked for long-term use (Cat.# J2962).

ワークフロー

従来法

Standard bioassay workflow with cultured cells
発光バイオアッセイ法(試薬として細胞も供給)
Improved bioassay workflow with thaw-and-use cells

標準化された試薬による最適な結果

  • ”解凍 & アッセイ” だけのフォーマットは従来法に比べ飛躍的に時間と労力を削減し、バラつきも低減
  • キットは標準化されたフォーマットとして必要なすべての試薬を供給
  • ハイスループットワークフローにも容易に適応

アッセイの原理

 
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Representation of the IL-15 Bioassay. The IL-15 Bioassay consists of a genetically engineered cell line, IL-15 Bioassay Cells. When IL-15 binds to its receptor, receptor-mediated signaling induces luminescence that can be detected by adding Bio-Glo™ Reagent and quantitated with  a luminometer. In the absence of IL-15, no signaling occurs downstream of IL-15R and a luminescent signal is not generated.

Assay Performance Data

Accuracy and Precision are critical to bioassays and the development of biologics. The IL-15 Bioassay is both accurate and precise. Accuracy is a measure of how close results are to a target or reference value. Precision is a measure of repeatability. This assay was developed and prequalified according to ICH guidelines for accuracy and precision, giving it the performance characteristics required for many applications of a bioassay used for antibody drug discovery research and development.

Accuracy: Measure of ability to replicate a reference or "target" value.

IntraLab Precision: Measure of repeatability from multiple assays of the same homogeneous sample under the same operating conditions over a short time (same analyst; same lab; same day).

Intermediate Precision: Measure of repeatability of multiple assays of the same homogeneous sample with different analysts or equipment or on different days.

Linearity: Ability of your assay to provide measured values that are proportional to the concentration of the sample.

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The IL-15 Bioassay Shows Precision, Accuracy and Linearity.

Parameter Results
Accuracy % Expected Relative Potency % Recovery
50 97.8
70 101.4
140 97.9
200 102.7
Repeatability (% CV) 100% (Reference) 1.47
Intermediate Precision (% CV)   6.75
Linearity (r2)   0.998
Linearity (y = mx + b)   y = 1.03x–2.79
A 50–200% theoretical potency series of recombinant human IL-15 was analyzed in triplicate in three independent experiments performed on three days by two analysts (for a total of six independent experiments). Bio-Glo™ Reagent was added and luminescence quantified using the GloMax® Discover System. Data were analyzed and relative potencies calculated after parallelism determination using JMP® software. Data were generated using thaw-and-use cells.
The IL-15 Bioassay response to recombinant IL-15.
The IL-15 Bioassay indicates stability.
15624ma
IL-15 Bioassay Cells were incubated with serial dilutions of recombinant IL-15. After a 6-hour incubation, Bio-Glo™ Reagent was added and luminescence was quantified using the GloMax® Discover System.
15451ma
Recombinant human IL-15 was heat stressed at 53°C for 0–24 hours prior to being tested in the IL-15 Bioassay. Bio-Glo™ Reagent was added and luminescence was quantified using the GloMax® Discover System.

Product Kit Formats and Related Products

  • IL-15 Bioassay is available in 1X and 5X sizes.
  • IL-15 Bioassay is also available in CPM format (Cat.# J2962).

製品仕様

You are viewing: JA2011 Change Configuration

選択製品の構成品内容

Item Part # Size

IL-15 Bioassay Cells

J298A 1 × 0.80ml

Fetal Bovine Serum

J121A 1 × 4ml

RPMI 1640 Medium

G708A 1 × 36ml

Bio-Glo™ Luciferase Assay Buffer

G719A 1 × 10ml

Bio-Glo™ Luciferase Assay Substrate

G720A 1 × 1 vial

分析証明書

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使用制限

Not For Medical Diagnostic Use.

保存条件

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,077,244 and other patents.

製品仕様

You are viewing: JA2015 Change Configuration

選択製品の構成品内容

Item Part # Size

IL-15 Bioassay Cells

J298A 5 × 0.80ml

Fetal Bovine Serum

J121A 5 × 4ml

RPMI 1640 Medium

G708A 5 × 36ml

Bio-Glo™ Luciferase Assay Buffer

G719A 5 × 10ml

Bio-Glo™ Luciferase Assay Substrate

G720A 5 × 1 vial

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,077,244 and other patents.

製品仕様

You are viewing: J2962 Change Configuration

選択製品の構成品内容

Item Part # Size

IL-15 Bioassay Cells (CPM)

J296A 2 × 0.75ml

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

XX

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells except that recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

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