IL-2Rβγ Bioassay

Part Numbers: JA5101, JA5105, J3392, GA1410

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免疫調節抗体の特性解析のための免疫チェックポイントバイオアッセイ

  • ICH ガイドラインに従って事前承認
  • 96- および 384-ウェルプレートフォーマット
  • 細胞の採取も不要

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選択製品のカタログ番号: JA5101

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IL-2Rβγ Bioassay
Thaw-and-Use/1X each component
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安定した結果が得られるシンプルな IL-2 バイオアッセイ

1976年に T-細胞成長因子として発見されたインターロイキン-2  (IL-2) は、CD4+および CD8+ T 細胞、ナチュラルキラー (NK) 細胞、活性化樹状細胞など様々な細胞種により分泌される単量体タンパク質(15.5kDa)です。 IL-2 は免疫システムに多面的に影響を与えています。また、 CD4+ 制御性 T 細胞の生成、維持、展開に重要な役割を果たしており、CD8+ NK 細胞の細胞毒性活性を促進し、活性化誘導型細胞死 を介して有害な自己反応性T細胞を除去して恒常性を維持しています。

IL-2 Bioassay は  STAT-5 応答配列を利用して IL-2 による刺激や阻害を測定するためにデザインされた細胞ベース発光アッセイです。IL-2 Bioassay には "thaw-and-use" の簡単フォーマットで使用できる凍結保存細胞が含まれており、細胞を融解した後、細胞を増やすことなくそのままアッセイに使用することができます。 IL-2 Bioassay には  融解して、増殖、バンキングが許容された凍結保存細胞を含む長期使用のための Cell Propagation Model (CPM) フォーマットもご利用いただけます (IL-2 Bioassay, Propagation Model, カタログ番号 J2952)。

アッセイの構成品

The IL-2Rβγ Bioassay cells included are provided in a thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell propagation (Cat.# JA5101, JA5105).

The IL-2Rβγ Bioassay is also available in a Cell Propagation Model (CPM) (Cat.# J3392) and Cell Bank format (Cat.# GA1410) as cryopreserved cells that can be thawed and propagated for long-term use.

ワークフロー

従来法

Standard bioassay workflow with cultured cells
発光バイオアッセイ法(試薬として細胞も供給)
Improved bioassay workflow with thaw-and-use cells

標準化された試薬による最適な結果

  • ”解凍 & アッセイ” だけのフォーマットは従来法に比べ飛躍的に時間と労力を削減し、バラつきも低減
  • キットは標準化されたフォーマットとして必要なすべての試薬を供給
  • ハイスループットワークフローにも容易に適応

アッセイの原理

The IL-2Rβγ Bioassay is a bioluminescent cell-based assay designed to measure human IL-2Rβγ receptor stimulation or inhibition.

How the IL-2Rβγ Bioassay works.

Representation of the IL-2Rβγ Bioassay. The IL-2Rβγ Bioassay consists of a genetically engineered cell line, IL-2Rβγ Bioassay Cells. When IL-2 or IL-15 bind to the medium-affinity CD122/CD132 heterodimeric receptor complex (IL-2Rβγ), receptor-mediated pathway signaling induces luminescence that can be detected upon addition of Bio-Glo-NL™ Reagent and quantified with a luminometer. In the absence of cytokine, no signaling occurs downstream of the CD122/CD132 complex and a luminescent signal is not generated.

アッセイパフォーマンスデータ

正確度 (accuracy) と 精度 (precision) はバイオアッセイやバイオロジックス開発において非常に重要です。IL-2 Bioassay は正確度と 精度を兼ね備えたアッセイです(正確度とは標的または参照値に対してどれほど近かったかという度合いであり、精度は再現性の度合いです)。本アッセイは正確度 と 精度 のためのICHガイドラインに従い開発、事前承認されており、抗体医薬の研究開発に使用するバイオアッセイの様々なアプリケーションに必要とされるパフォーマンス特性を備えています。

32454752-data-target-combined

The IL-2Rβγ Bioassay Shows Precision, Accuracy and Linearity.

Parameter Results
Accuracy
% Expected Relative Potency
% Recovery
50
90.3
75
102.6
125
105.7
150
101.7
Repeatability (% CV)
100% (Reference)
3.6
Intermediate Precision (% CV)  
8.4
Linearity (r2)  
0.996
Linearity (y = mx + b)  
y = 1.08x – 6.35
A 50–150% theoretical potency series of recombinant human IL-15 was analyzed in triplicate in three independent experiments performed on three days by two analysts (for a total of six independent experiments). Bio-Glo-NL™ Reagent was added and luminescence quantified using the GloMax® Discover System. Data were analyzed and relative potencies calculated after parallelism determination using JMP® software. Data were generated using thaw-and-use cells.
Representative data for the IL-2Rβγ Bioassay; EC50 = 81ng/ml.
18834ma-c

Representative data for the IL-2Rβγ Bioassay. IL-2Rβγ Bioassay cells were prepared as described in the protocol and incubated with serial dilutions of recombinant IL-2 and IL-15. After a 6-hour incubation, Bio-Glo-NL™ Reagent was added, and luminescence was quantified using the GloMax® Discover System. Data were fitted to a 4PL curve using GraphPad Prism® software. The IL-2 EC50 was 81ng/ml (Panel A), with a fold induction of approximately 24 (Panel B). The IL-15 EC50 was 4.4ng/ml with a fold induction of approximately 28. Data were generated using cells from culture. Panel A shows raw luminescence measurements. Panel B displays the calculated fold induction.

製品仕様

You are viewing: JA5101 Change Configuration

選択製品の構成品内容

Item Part # Size

IL-2Rβγ Bioassay Cells

J340A 1 × 0.6ml

Fetal Bovine Serum

J121A 1 × 4ml

RPMI 1640 Medium

G708A 1 × 36ml

Bio-Glo-NL™ Luciferase Assay Buffer

J308A 1 × 10ml

Bio-Glo-NL™ Luciferase Assay Substrate

J309A 1 × 200μl

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

製品仕様

You are viewing: JA5105 Change Configuration

選択製品の構成品内容

Item Part # Size

IL-2Rβγ Bioassay Cells

J340A 5 × 0.6ml

Fetal Bovine Serum

J121A 5 × 4ml

RPMI 1640 Medium

G708A 5 × 36ml

Bio-Glo-NL™ Luciferase Assay Buffer

J308A 5 × 10ml

Bio-Glo-NL™ Luciferase Assay Substrate

J309A 5 × 200μl

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

製品仕様

You are viewing: J3392 Change Configuration

選択製品の構成品内容

Item Part # Size

IL-2Rβγ Bioassay Cells (CPM)

J339A 2 × 1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

XX

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells except that recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

製品仕様

You are viewing: GA1410 Change Configuration

選択製品の構成品内容

Item Part # Size

IL-2Rβγ Bioassay Cells (CPM)

J339A 50 × 1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

XX

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells except that recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

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