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T Cell Activation Bioassay (NFAT/IL-2)

Part Numbers: J1621, J1625, J1601, J1651, J1655, J1631

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T 細胞の応答性に作用するバイオロジックスの安定性および精度を測定

  • ICH ガイドラインの事前承認済み
  • ハイスループットフォーマットも可能
  • 細胞培養も不要

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選択製品のカタログ番号: J1621

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T Cell Activation Bioassay (NFAT/IL-2)
NFAT-RE Kit/1X each component
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発光法による 細胞ベースの T 細胞活性化アッセイ

T細胞応答性の誘導、増強、改変を目的とした免疫療法戦略は、がんや自己免疫疾患などの治療法として有望なアプローチとして注目を集めています。T細胞の活性化はTCR/CD3複合体と共刺激受容体の協調により開始されます。TCR/CD3の協調はNFAT経路を活性化し、TCR/CD3 + CD28の相互作用はNFAT, AP-1 および NF-κBの各径路を活性化しIL-2の産生を誘導します。

T Cell Activation Bioassay は従来のアッセイ法の限界を超える発光法による細胞ベースのアッセイで、二重特異性抗体やCAR-T 細胞療法など新しいバイオロジックスの発見、開発に使用することができます。

 【かわら版】プライマリー細胞不要! T 細胞活性化バイオアッセイ

 

T細胞応答性の誘導、増強、改変を目的とした免疫療法戦略は、がんや自己免疫疾患などの治療法として有望なアプローチとして注目を集めています。T細胞の活性化はTCR/CD3複合体と共刺激受容体の協調により開始されます。TCR/CD3の協調はNFAT経路を活性化し、TCR/CD3 + CD28の相互作用はNFAT, AP-1 および NF-κBの各径路を活性化しIL-2の産生を誘導します。

T Cell Activation Bioassayは従来のアッセイ法の限界を超える発光法による細胞ベースのアッセイで、二重特異性抗体やCAR-T 細胞療法など新しいバイオロジックスの発見、開発に使用することができます。このアッセイにはNFAT応答配列 (NFAT-RE) またはIL-2プロモーターのいずれかにより駆動するルシフェラーゼレポーター(TCR/CD3 Effector Cells) を発現する遺伝子改変Jurkat T 細胞株などで構成されています。
T-Cell-Activation-Bioassays-Overview-Image

T Cell Activation Bioassay の概要

13791MA_W
The T Cell Activation Bioassay (NFAT and IL-2) each consists of a genetically engineered cell line, TCR/CD3 Effector Cells NFAT (Panel A) or IL-2 (Panel B). When engaged with either an anti-TCR/CD3 stimulus alone or an anti-TCR/CD3 and an anti-CD28 stimulus, receptor-mediated signaling induces luminescence.

アッセイ原理

このアッセイにはNFAT応答配列 (NFAT-RE) または IL-2 プロモーターのいずれかにより駆動するルシフェラーゼレポーター ( TCR / CD3 Effector Cells ) を発現する遺伝子改変 Jurkat T 細胞株などで構成されています。TCR / CD3 Effector Cells ( NFAT ) が適切な TCR / CD3 リガンドあるいは anti- TCR / CD3 抗体が結合すると TCR が細胞内シグナルに変換し NFAT - REを介して発光を生じます。同様に TCR / CD3 Effector Cells ( IL-2 ) が anti-TCR / CD3 抗体および anti - CD28  抗体による刺激を受けると受容体を介したシグナルが誘導され IL - 2 プロモーターを介した発光を生じます。

事前承認済みのアッセイ

ICHガイドラインではバイオアッセイは事前認定され、有効性や安定性検査での常用に耐える精度、正確度、直線性を示します。バイオアッセイのワークフローはシンプル、頑健で96ウェルおよび384ウェルプレートフォーマットでの抗体スクリーニングおよび薬剤開発にも使用されます。さらにバイオアッセイはヒトの血清にも耐え、中和抗体バイオアッセイ開発への応用も期待できます。

利点

ICHガイドラインに準じたバイオアッセイ: 力価および安定性検査におけるルーチンでの使用に要求される正確度、精度および直線性を示すバイオアッセイ

シンプルで頑健なワークフロー: 特別なスキルやトレーニングが不要なので導入が容易

抗体スクリーニングや薬剤開発も可能: 96ウェル/384ウェルプレートで行えるバイオアッセイ

複数の製品フォーマット: 実験やワークフローに合わせて選択可能

アプリケーション

抗CD3 二重特異性抗体の活性および特異性の検証

二重特異性抗体の力価および安定性測定

CAR-T 細胞受容体の活性測定

抗体の中和活性の測定

多様で便利な製品形態が利用可能

 

製品の構成品とフォーマット

T Cell Activation Bioassay Kits の構成品: TCR/CD3 Effector Cells (NFAT or IL-2)、Cell Culture Medium、Assay Reagents

キットの種類: 1 × components (1X) および 5 × components (5X) の2種類

製品仕様

You are viewing: J1621 Change Configuration

選択製品の構成品内容

Item Part # Size

TCR/CD3 Effector Cells (NFAT)

J133A 1 × 0.8ml

Fetal Bovine Serum

J121A 1 × 4ml

RPMI 1640 Medium

G708A 1 × 36ml

Bio-Glo™ Luciferase Assay Buffer

G719A 1 × 10ml

Bio-Glo™ Luciferase Assay Substrate

G720A 1 × 1 vial

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,077,244 and other patents.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

製品仕様

You are viewing: J1625 Change Configuration

選択製品の構成品内容

Item Part # Size

TCR/CD3 Effector Cells (NFAT)

J133A 5 × 0.8ml

Fetal Bovine Serum

J121A 5 × 4ml

RPMI 1640 Medium

G708A 5 × 36ml

Bio-Glo™ Luciferase Assay Buffer

G719A 5 × 10ml

Bio-Glo™ Luciferase Assay Substrate

G720A 5 × 1 vial

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,077,244 and other patents.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

製品仕様

You are viewing: J1601 Change Configuration

選択製品の構成品内容

Item Part # Size

TCR/CD3 Effector Cells (CPM) (NFAT)

J131A 2 × 1ml

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

XX

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient may genetically engineer the cell line using exogenous nucleic acid sequences provided by Researcher. In addition, recipient may propagate and store the cells for long-term use. Recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

製品仕様

You are viewing: J1651 Change Configuration

選択製品の構成品内容

Item Part # Size

TCR/CD3 Effector Cells (IL-2)

J129A 1 × 0.8ml

Fetal Bovine Serum

J121A 1 × 4ml

RPMI 1640 Medium

G708A 1 × 36ml

Bio-Glo™ Luciferase Assay Buffer

G719A 1 × 10ml

Bio-Glo™ Luciferase Assay Substrate

G720A 1 × 1 vial

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,077,244 and other patents.

製品仕様

You are viewing: J1655 Change Configuration

選択製品の構成品内容

Item Part # Size

TCR/CD3 Effector Cells (IL-2)

J129A 5 × 0.8ml

Fetal Bovine Serum

J121A 5 × 4ml

RPMI 1640 Medium

G708A 5 × 36ml

Bio-Glo™ Luciferase Assay Buffer

G719A 5 × 10ml

Bio-Glo™ Luciferase Assay Substrate

G720A 5 × 1 vial

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,077,244 and other patents.

製品仕様

You are viewing: J1631 Change Configuration

選択製品の構成品内容

Item Part # Size

TCR/CD3 Effector Cells (CPM) (IL-2)

J127A 2 × 1ml

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

XX

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient may genetically engineer the cell line using exogenous nucleic acid sequences provided by Researcher. In addition, recipient may propagate and store the cells for long-term use. Recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

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