Riboprobe® Combination Systems
Produce High-Specific-Activity RNA Probes or Defined RNA Transcripts
- Choose from combination systems with T3/T7 or SP6/T7 RNA polymerases
- SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template
- Allows synthesis of RNA corresponding to the coding or noncoding strand of cloned DNA from a single plasmid construct
Catalog Number:
Choose a system
Catalog Number: P1450
Catalog Number: P1460
The Riboprobe® Combination Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. The Riboprobe® Combination Systems include the RNA polymerases, all of the required reagents (excluding radioisotope) for performing transcription reactions in vitro and RQ1 RNase-Free DNase (Cat.# M6101) for removal of the template following transcription.
Applications of the Riboprobe® Systems include:
- Generation of high-specific-activity RNA probes.
- Generation of microgram quantities of RNA transcripts.
- Generation of RNA processing substrates.
- Generation of antisense RNA.
Looking for a scalable IVT solution with animal-origin-free reagents? The RiboMAX™ AOF RNA Production System supports RNA synthesis from research to production.
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
RQ1 RNase-Free DNase |
M610C | 1 × 110u | View Product | |
|
Recombinant RNasin® Ribonuclease Inhibitor |
N251A | 1 × 2,500u | 40u/μl | View Product |
rATP |
P113A | 2 × 50μl | 10mM | View Product |
rCTP |
P114A | 2 × 50μl | 10mM | View Product |
rGTP |
P115A | 2 × 50μl | 10mM | View Product |
rUTP |
P116A | 2 × 50μl | 10mM | View Product |
|
DTT |
P117A | 2 × 100μl | 100mM | View Product |
|
Transcription Optimized 5X Buffer |
P118B | 1 × 500μl | View Product | |
|
Nuclease-Free Water |
P119A | 2 × 1,250μl | View Product | |
|
T7 RNA Polymerase |
P207D | 1 × 500u | View Product | |
|
T3 RNA Polymerase |
P208A | 1 × 500u | View Product | |
|
pGEM® Express Positive Control Template |
P256A | 1 × 5μg | View Product |
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
RQ1 RNase-Free DNase |
M610C | 1 × 110u | View Product | |
|
Recombinant RNasin® Ribonuclease Inhibitor |
N251A | 1 × 2,500u | 40u/μl | View Product |
rATP |
P113A | 2 × 50μl | 10mM | View Product |
rCTP |
P114A | 2 × 50μl | 10mM | View Product |
rGTP |
P115A | 2 × 50μl | 10mM | View Product |
rUTP |
P116A | 2 × 50μl | 10mM | View Product |
|
DTT |
P117A | 2 × 100μl | 100mM | View Product |
|
Transcription Optimized 5X Buffer |
P118B | 1 × 500μl | View Product | |
|
Nuclease-Free Water |
P119A | 2 × 1,250μl | View Product | |
|
SP6 RNA Polymerase |
P108E | 1 × 500u | View Product | |
|
T7 RNA Polymerase |
P207D | 1 × 500u | View Product | |
|
pGEM® Express Positive Control Template |
P256A | 1 × 5μg | View Product |
Resources
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