Riboprobe® Systems
Produce High-Specific-Activity RNA Probes or Defined RNA Transcripts
- Systems available with SP6 RNA Polymerase, T7 RNA Polymerase or T3 RNA Polymerase
- SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template
- Allow synthesis of RNA corresponding to the coding or noncoding strand of cloned DNA from a single plasmid construct
Catalog Number:
Choose a system
Catalog Number: P1420
Catalog Number: P1430
Catalog Number: P1440
The Riboprobe® Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. These systems contain all components necessary for in vitro transcription from a DNA template (excluding the radioisotope) and also contain RQ1 RNase-Free DNase (Cat.# M6101) for template removal following transcription.
Applications of the Riboprobe® Systems include:
- Generation of high-specific-activity RNA probes.
- Generation of microgram quantities of RNA transcripts.
- Generation of RNA processing substrates.
- Generation of antisense RNA.
Looking for a scalable IVT solution with animal-origin-free reagents? The RiboMAX™ AOF RNA Production System supports RNA synthesis from research to production.
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
RQ1 RNase-Free DNase |
M610C | 1 × 110u | View Product | |
|
Recombinant RNasin® Ribonuclease Inhibitor |
N251A | 1 × 2,500u | 40u/μl | View Product |
|
SP6 RNA Polymerase |
P108E | 1 × 500u | View Product | |
rATP |
P113A | 1 × 50μl | 10mM | View Product |
rCTP |
P114A | 1 × 50μl | 10mM | View Product |
rGTP |
P115A | 1 × 50μl | 10mM | View Product |
rUTP |
P116A | 1 × 50μl | 10mM | View Product |
|
DTT |
P117A | 1 × 100μl | 100mM | View Product |
|
Transcription Optimized 5X Buffer |
P118B | 1 × 500μl | View Product | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product | |
|
pGEM® Express Positive Control Template |
P256A | 1 × 5μg | View Product |
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
RQ1 RNase-Free DNase |
M610C | 1 × 110u | View Product | |
|
Recombinant RNasin® Ribonuclease Inhibitor |
N251A | 1 × 2,500u | 40u/μl | View Product |
rCTP |
P114A | 1 × 50μl | 10mM | View Product |
rATP |
P113A | 1 × 50μl | 10mM | View Product |
rGTP |
P115A | 1 × 50μl | 10mM | View Product |
rUTP |
P116A | 1 × 50μl | 10mM | View Product |
|
DTT |
P117A | 1 × 100μl | 100mM | View Product |
|
Transcription Optimized 5X Buffer |
P118B | 1 × 500μl | View Product | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product | |
|
T3 RNA Polymerase |
P208A | 1 × 500u | View Product | |
|
pGEM® Express Positive Control Template |
P256A | 1 × 5μg | View Product |
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
RQ1 RNase-Free DNase |
M610C | 1 × 110u | View Product | |
|
Recombinant RNasin® Ribonuclease Inhibitor |
N251A | 1 × 2,500u | 40u/μl | View Product |
rATP |
P113A | 1 × 50μl | 10mM | View Product |
rCTP |
P114A | 1 × 50μl | 10mM | View Product |
rGTP |
P115A | 1 × 50μl | 10mM | View Product |
rUTP |
P116A | 1 × 50μl | 10mM | View Product |
|
DTT |
P117A | 1 × 100μl | 100mM | View Product |
|
Transcription Optimized 5X Buffer |
P118B | 1 × 500μl | View Product | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product | |
|
T7 RNA Polymerase |
P207D | 1 × 500u | View Product | |
|
pGEM® Express Positive Control Template |
P256A | 1 × 5μg | View Product |
Resources
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Riboprobe® Combination Systems
SP6/T7 または T3/T7 システムの各組合わせシステムより高比活性の RNA プローブまたは転写物を合成
P1450, P1460
Riboprobe® System Components and Buffers
in vitro 転写に最適化されたバッファー、rNTPs および Nuclease-Free Water のパッケージ
P1121
RiboMAX™ Large-Scale RNA Production Systems
in vitro または in vivo 翻訳のためにキャップあり（またはキャップなし）の RNA 転写物を合成
P1280, P1300
T7 RiboMAX™ Express Large-Scale RNA Production System
Designed for speed. Produces 5–8.5mg/ml RNA in 30 minutes.
P1320
関連製品
pGEM® Express Positive Control Template
Riboprobe® Systems を用いた in vitro 転写反応のモニタリングに使用
P2561
PureYield™ Plasmid Midiprep System
50ml培養液から30分でトランスフェクション品質のプラスミドを最大200µg精製
A2492, A2495, A2496
Set of rATP, rCTP, rUTP, rGTP
10mM または 100mM の rNTP セット
P1221, E6000
T7 RNA Polymerase
T7 プロモーター配列に極めて高い特異性を示すDNA依存性RNAポリメラーゼ
P2075, P2077, P4074, P618X, P617X
