T7 RiboMAX™ Express Large Scale RNA Production System

Part Numbers: P1320

P1320_T7-RiboMAX--Express-Large-Scale-RNA-Pro_3
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Produces 5–8.5mg/ml RNA in 30 Minutes

  • Consistent production of milligram amounts of RNA
  • Minimal pipetting steps and setup time
  • Efficiently transcribes DNA templates of varying sizes. Works with transcripts as short as 21bp.
  • Animal origin-free (AOF) and cGMP-manufactured version available for RNA therapeutic applications. Learn more.

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選択製品のカタログ番号: P1320

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T7 RiboMAX™ Express Large Scale RNA Production System
1 system
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Quickly Produce Milligram Amounts of RNA

T7 RiboMAX™ Express Large Scale RNA Production System は、in vitro または in vivo での翻訳に適したキャップの無い RNA 転写物を迅速に大量に生成するシステムです。T7 RiboMAX™ Express Large Scale RNA Production System で合成した RNA は、標準的な方法で合成した RNA よりもウサギ網状赤血球由来の in vitro 翻訳システムで優れた性能を発揮します。これは、通常in vitro翻訳を阻害する高濃度のRNAで特に顕著です。

この T7 RiboMAX™ Express Large Scale RNA Production System は、ピペッティングのステップを最小限に抑えた簡便なセットアップを可能とし、効率を高めるとともに潜在的なエラーを低減します。このシンプルなセットアップを提供するために、4つの rNTPs をすべて含む 2X transcription buffer が供給されます。また、転写後のプラスミドテンプレートを分解するためのRQ1 RNase-Free DNaseも付属しています。

2X バッファーと rNTPの混合液として提供されるため、T7 RiboMAX™ Express システムはキャップ付き RNA を必要とするアプリケーション用のRNA 合成には推奨されません。キャップ付きRNAの合成には、標準の RiboMAX™ Large Scale RNA Production System—T7 (Cat.# P1300) をお選びください。

T7 RiboMAX™ Express システムの用途 :

  • RT-qPCR アッセイ用のスタンダードの調製
  • 非哺乳類 RNA 干渉研究用の二本鎖 RNA および長鎖 RNA の調製
  • tRNA および rRNA、RNA ウイルスゲノム、リボザイムの in vitro 合成
  • RNAスプライシング、RNA二次構造、RNA:タンパク質相互作用の研究用基質の調製
  • アンチセンス RNA の合成
RNA yield from RiboMax Express RNA production system

RNA yield. The RNA yield from T7 RiboMAX™ Express in vitro transcription reactions incubated for the indicated time periods was determined by UV absorbance at 260nm. The numbers on top of each bar represent the RNA yield in micrograms resulting from in vitro transcription reactions with a volume of 20µl.

Analysis of in vitro transcripts by agarose gel electrophoresis.
Analysis of T7 RiboMAX™ Express-generated in vitro transcripts. In vitro transcribed RNAs were visualized by agarose (1%) gel electrophoresis in 1X TBE/0.1% SDS buffer and subsequent ethidium bromide staining. Each lane contains 1μg of RNA resulting from in vitro transcription reactions incubated for the indicated time periods. Full-length RNA of 27.3kb is indicated. DNA fragment sizes of 500ng of EcoRI- and HindIII-digested bacteriophage lambda DNA (lane M) are shown.

文献紹介

Development of mRNA manufacturing for vaccines and therapeutics: mRNA platform requirements and development of a scalable production process to support early phase clinical trials.

Duke Human Vaccine Institute at Duke University School of Medicine and Perelman School of Medicine at University of Pennsylvania together developed a scalable, GMP-compatible process for production of mRNA vaccines and therapeutics using Promega RiboMAX-related products.

文献を読む

Looking for a scalable IVT solution with animal-origin-free reagents? The RiboMax™ AOF RNA Production System supports RNA synthesis from research to production.

製品仕様

選択製品の構成品内容

Item Part # Size Available Separately

RQ1 RNase-Free DNase

M610C 1 × 110u View Product

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Enzyme Mix, T7 Express

P132A 1 × 100μl

Sodium Acetate, 3.0M (pH 5.2)

P135A 1 × 1ml

RiboMAX™ Express T7 2X Buffer

P164A 1 × 500μl

pGEM® Express Positive Control Template

P256A 2 × 5μg View Product

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

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