BCAA-Glo™ Assay Technical Manual
Instructions for Use of Product(s)
JE9300, JE9400
Literature # TM712
The BCAA-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of branched-chain amino acids (BCAA) in biological samples. The term branched-chain amino acids refers to three amino acids: leucine, isoleucine and valine. BCAA participate in many central cell functions, including protein synthesis and metabolism, and elevated serum levels have been found to correlate with metabolic diseases such as diabetes.
The BCAA-Glo™ Assay couples BCAA oxidation and NADH production with a bioluminescent NADH detection system. When BCAA detection reagent is added to a sample at a 1:1 ratio, the coupled-enzyme reactions are initiated and run simultaneously. The luminescent signal is proportional to the amount of BCAA in the sample, and increases until all BCAA is consumed, at which point a stable luminescent signal is achieved.
Summary of Changes
The following changes have been made to the 7/24 revision of this document:
1. In Section 5.A, two Thermo Fisher Scientific catalog numbers were updated.
2. Third party trademarks were added or updated.
Revised 7/24.