HiBiT ブロッティング : ウェスタンブロットに代わる迅速で高感度な方法

たったの5分で結果が得られます！
抗体を使わない検出法
高感度 & 低バックグラウンド
洗浄操作やブロッキングも不要

How Does HiBiT Compare With Other Epitope Tags?

HiBiT is an 11-amino acid epitope tag enabling high-throughput, live-cell protein quantification via ultra-sensitive bioluminescence detection. The high-affinity Anti-HiBiT Monoclonal Antibody also enables applications like immunoprecipitation-mass spectrometry at endogenous levels. Read more about the advantages of HiBiT in this article: "HiBiT: A Tiny Tag Expanding Endogenous Protein Detection".

Get HiBiT Sequence

To obtain the HiBiT sequence, you must review the terms and conditions for use.

Tag	Size	Detection Method	Primary Applications	Key Advantages	Why choose HiBiT?
HiBiT	Length: 11 aa MW: 1.2KDa	Bioluminescence, enzyme complementation, low picomolar/high-affinity monoclonal antibody	Total protein quantification, live-cell kinetic quantification, surface/secreted protein quantification, Western blot, flow cytometry, (Co-) immunoprecipitation, immunofluorescence, protein interactions	Ultra-sensitive, high-throughput quantitation, compatible with CRISPR for endogenous tagging, multifunctional, small size, high specificity, high affinity	A compact epitope tag enabling high-throughput, sensitive protein quantitation and traditional antibody-based methods at endogenous levels—simplifying workflows without bulky tags.
GFP	Length: 238 aa MW: 26.9Kda	Fluorescence	Protein localization, live-cell imaging, flow cytometry, detection and quantitation	Strong visual for localization in live cells	HiBiT is smaller, quantitative, and has less background with lower risk of disrupting function.
FLAG®	Length: 8 aa MW: 1KDa	Antibodies	Immunopurification, Western blot, (Co)-Immunoprecipitation, immunofluorescence, flow cytometry	Small size, many antibodies available	HiBiT offers antibody-free quantitation with a broader application range in live-cell studies.
HA	Length: 9 aa MW: 1.1KDa	Antibodies	(Co)-Immunoprecipitation, immunofluorescence, western blot, flow cytometry	Small size, many antibodies available	HiBiT provides higher throughput, quantitative detection, and live-cell applications.
ALFA-tag	Length: 13 aa MW: 1.4KDa	Single domain antibodies (VHH nanobodies)	Detection, purification, (Co)-Immunoprecipitation, protein interactions	Small size, high specificity, high affinity	HiBiT is a smaller, highly sensitive tool for accurate quantitation of low-abundance proteins with clean luminescence and minimal background, eliminating the need for SPR or Biacore™ measurements.
Myc	Length: 10 aa MW: 1.2KDa	Antibodies	(Co)-Immunoprecipitation, immunofluorescence, Western blot, flow cytometry	Small size, many antibodies available	HiBiT offers superior sensitivity, with quantitative and real-time monitoring.

Need A High-Sensitivity Alternative to Traditional Tags?

The Anti-HiBiT Monoclonal Antibody detects sub-picogram levels of HiBiT with minimal background, enabling applications like Western blotting, immunoprecipitation, and immunofluorescence. This antibody supports rapid and reliable verification of protein expression, size, and localization, making HiBiT tag an ideal choice for precise and efficient protein analysis.

View Anti-HiBiT Monoclonal Antibody