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CloneWeaver® Workflow Purchasing Tool

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Vectors: Bacterial Change

Name Description Part Number
pGEM-5Zf(+) Vector Open/Close Add
Blue/white screening to easily identify recombinant clones
Multiple cloning site provides a convenient selection of restriction sites for cloning

標準的なクローニングベクターあるいはin vitro転写用テンプレートとして使用できます。また、フィラメントファージf1の複製開始点が含まれるため、環状のssDNAを調製できます。Erase-a-Base®Systemでの使用に便利な制限酵素サイト(両外側には3'突出末端、内側には5'突出末端あるいは平滑末端を生じる制限酵素サイト)が含まれます。f1オリジンの方向性を除き、pGEM®-5Zf(+)VectorとpGEM®-5Zf(–)Vectorはすべて同一です。lacZ α-ペプチド配列が含まれるため、青/白スクリーニングにより組換え体を選択できます。

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pGEM-7Zf(+) Vector Open/Close Add
Blue/white screening for easily identifying recombinant clones
In vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
Multiple cloning region provides a convenient selection of restriction sites for cloning

標準的なクローニングベクターあるいはin vitro転写用テンプレートとして使用できます。また、フィラメントファージf1の複製開始点が含まれるため、環状のssDNAを調製できます。Erase-a-Base®Systemでの使用に便利な制限酵素サイト(両外側には3'突出末端、内側には5'突出末端あるいは平滑末端を生じる制限酵素サイト)が含まれます。f1オリジンの方向性を除き、pGEM®-7Zf(+)VectorとpGEM®-7Zf(–)Vectorはすべて同一です。lacZ α-ペプチド配列が含まれるため、青/白スクリーニングにより組換え体を選択できます。

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