Promega Restriction Enzyme Tool

You can find an enzyme by entering the name, recognition sequence or overhang sequence. Promega enzymes are indicated in orange.

Search by Name

Select "Name", and enter the name of your enzyme into the search box.

Search by Sequence

Select "Sequence", and enter the recognition sequence into the search box. Only exact matches will be returned when the "wildcard match" is turned OFF. The following IUPAC codes can be used when the wildcard match is turned ON:

N = A,T,C or G (any nucleotide)
Y = T or C (pyrimidine)
R = G or A (purine)
M = A or C (amino)
K = G or T (keto)
S = G or C (strong interactions: 3 H bonds)
W = A or T (weak interactions: 2 H bonds)
B = G, T or C (not A)
V = G, C or A (not T or U)
D = G, A or T (not C)
H = A, C or T (not G)

Search by Overhang

Search by specific 3' or 5' overhang, or list all blunt enzymes.

Find Buffers For Double Digests

Once you have selected an enzyme, you can select a second enzyme and retrieve information on suitable buffers for double digests. Activity data in Promega buffers are not available for all enzymes.

Recognition sequence, cut site and overhang are indicated for each enzyme.

If the enzyme has isoschizomers (enzymes with the same recognition sequence and cut site) or neoschizomers (enzymes with the same recognition sequence but a different cut site), a list of these enzymes is provided. Select any of the listed enzymes to see further details on cut site, overhang and required incubation conditions.

For Promega enzymes, the incubation temperature, supplied buffer, and activity in other Promega buffers is provided.

A red background indicates that the enzyme/buffer combination exhibits star activity.

For enzymes that have been tested in Promega buffers, you can find information on suitable buffers to use for double digests. To use the double digest tool:

1. Select an acceptable co-activity level.
2. Choose a second enzyme from the list of enzymes with the selected level of activity in the compatible buffer.
3. The display will show the range of buffer choices for a double digest with both selected enzymes.
4. If no suitable enzyme with the selected co-activity is found, you can adjust the co-activity level to expand the list of enzyme choices.

A red background indicates that the enzyme/buffer combination exhibits star activity.