| Cell Line |
Transfection Reagent |
Type of Nucleic Acid Transfected |
Plate Format |
Amount of Nucleic Acid Used (µg) |
Ratio of Reagent to Nucleic Acid (µl:µg) |
Journal Citation |
MCF7 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
2 |
3:2 |
2012. PLoS ONE 7: e37255 |
Description of cell line: human breast adenocarcinoma
Additional Relevant Information: 50% confluent at transfection
Type of Transfection:
|
 |
MCF7 |
FuGENE 6 Transfection Reagent |
DNA |
not reported |
not reported |
3:1 |
2001. J. Biol. Chem. 276: 39892-7 |
Description of cell line: human breast adenocarcinoma
Additional Relevant Information: 70% efficiency reported
Type of Transfection:
|
|
MCF7 |
FuGENE 6 Transfection Reagent |
DNA |
not reported |
not reported |
6:4 |
2001. J. Biol. Chem. 276: 47038-45 |
Description of cell line: human breast adenocarcinoma
Additional Relevant Information:
Type of Transfection:
|
|
MCF7 |
FuGENE HD Transfection Reagent |
DNA |
35mm dish |
4 |
2:1 |
2009. Cancer Epidemiol. Biomarkers 18: 2468-75 |
Description of cell line: human breast adenocarcinoma
Additional Relevant Information: 80% confluent at transfection
Type of Transfection:
|
|
MDCK |
FuGENE 6 Transfection Reagent |
DNA |
60 mm dish |
2.05 or 4.05 |
3:1 |
|
Description of cell line: Madin Darby canine kidney
Additional Relevant Information:
Type of Transfection:
|
|
MEF |
FuGENE HD Transfection Reagent |
DNA |
12-well plate |
2 |
not reported |
2012. Mol. Biol. Cell 23: 3499-510 |
Description of cell line: mouse embryo fibroblasts
Additional Relevant Information: 5x10e4 cells/well plated 1 day prior to transfection
Type of Transfection:
|
|
MEF |
FuGENE HD Transfection Reagent |
DNA |
6-well plate |
3 |
3:1 |
2012. J. Virol. 86: 8198–8209 |
Description of cell line: mouse embryo fibroblasts
Additional Relevant Information:
Type of Transfection:
|
|
MLE-15 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
2 |
3:1 |
2000. J. Virol. 74: 5776–87 |
Description of cell line: mouse transgenic lung
Additional Relevant Information: 2x10e5 to 4x10e5 cells plated 24hr prior to transfection
Type of Transfection:
|
|
mtCC1-2 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
2 |
3:1 |
2000. J. Virol. 74: 5776–87 |
Description of cell line: mouse myeloma
Additional Relevant Information: 2x10e5 to 4x10e5 cells plated 24 hr prior to transfection
Type of Transfection:
|
|
NHBE |
FuGENE 6 Transfection Reagent |
siRNA |
12-well plate |
0.1nmole |
not reported |
2006. Am. J. Respir. Cell Mol. Biol. 34: 119–27 |
Description of cell line: normal human bronchial epithelial cells
Additional Relevant Information: 60-75% confluent, for each well 2ul of FuGene 6 was added to 48ul of media, vortex, RT/5min, added 0.1nmole annealed siRNA, vortexed, RT/15min. 50ul added to each well- final concentration was 0.45% FuGene and 225nM siRNA
Type of Transfection:
|
|
PC12 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
1 |
3:1 |
|
Description of cell line: rat adrenal gland
Additional Relevant Information: 50-60% confluence at time of transfection
Type of Transfection:
|
|
PC12 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
2 |
3:1 |
2002. J. Biol. Chem. 277: 40919-29 |
Description of cell line: rat adrenal gland
Additional Relevant Information: 50-60% confluence at time of transfection
Type of Transfection:
|
|
PC3 |
FuGENE 6 Transfection Reagent |
DNA |
24-well plate |
0.5 |
not reported |
2007. Anticancer Res. 27: 3843–8 |
Description of cell line: human prostate cancer
Additional Relevant Information: cells plated at 3x10e4 cells/well
Type of Transfection:
|
|
rat neonatal cardiomyocytes |
FuGENE 6 Transfection Reagent |
DNA |
8 well glass slide |
3 |
4:1 |
2000. J. Biochem. 275: 19250–7 |
Description of cell line: ventricular myocytes from neonatal rats
Additional Relevant Information: 20mm chamber slide was used
Type of Transfection:
|
|
rat neonatal cardiomyocytes |
FuGENE 6 Transfection Reagent |
DNA |
8 well glass slide |
3 |
4:1 |
2000. J. Biochem. 275: 19250–7 |
Description of cell line: ventricular myocytes from neonatal rats
Additional Relevant Information: 20mm chamber slide was used
Type of Transfection:
|
|
RAW264-7 |
FuGENE 6 Transfection Reagent |
DNA |
60 mm dish |
4.5 |
3.3:1 |
2000. J. Biol. Chem. 275: 9841-8 |
Description of cell line: mouse macrophage, Abelson murine leukemia virus-induced tumor
Additional Relevant Information:
Type of Transfection:
|
|
RAW264-7 |
FuGENE 6 Transfection Reagent |
DNA |
12-well plate |
not reported |
3:1 |
2005. J. Lipid Res. 46: 2151–67 |
Description of cell line: mouse macrophage, Abelson murine leukemia virus-induced tumor
Additional Relevant Information: 3-5x10e5 cells plated/well, transfection mix included 0.05ug of control vector
Type of Transfection:
|
|
RAW264-7 |
FuGENE 6 Transfection Reagent |
DNA |
12-well plate |
0.5 |
3:1 |
2012. J. Biol. Chem. 287: 3217–3230 |
Description of cell line: mouse macrophage, Abelson murine leukemia virus-induced tumor
Additional Relevant Information: 1.5X10e5 cells plated/well
Type of Transfection:
|
|
RAW264-7 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
not reported |
8:1 |
2005. J. Biol. Chem. 280: 39950–39961 |
Description of cell line: mouse macrophage, Abelson murine leukemia virus-induced tumor
Additional Relevant Information: Cells transfected at 75% confluency
Type of Transfection:
|
|
S2Rplus |
FuGENE 6 Transfection Reagent |
DNA |
12-well plate |
0.6 |
8.3:1 |
2011. Mol. Biol. Cell 22: 2834-47 |
Description of cell line: drosophila Schneider cell line
Additional Relevant Information: 0.6x10e6 cells plated 1 day prior to transfection
Type of Transfection:
|
|