Transfection Assistant Results

Cell Line

Transfection Reagent

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Cell Line Transfection Reagent Type of Nucleic Acid Transfected Plate Format Amount of Nucleic Acid Used (µg) Ratio of Reagent to Nucleic Acid (µl:µg) Journal Citation
AC29
FuGENE-6-Transfection-Reagent DNA 35 mm dish 1 6:1
AML12
FuGENE-6-Transfection-Reagent DNA 35 mm dish 2.4 not reported
AR42J-B13
FuGENE-6-Transfection-Reagent DNA 35 mm dish 1.5 not reported 2000. Nat. Cell Biol. 2: 879-887
AR42J-B13
FuGENE-6-Transfection-Reagent DNA 35 mm dish 2 not reported 2000. Nat. Cell Biol. 2: 879-887
As4.1
FuGENE-6-Transfection-Reagent DNA 12-well plate 2.3 2:1 1998. J. Biol. Chem. 273: 25292-300
BE
FuGENE-6-Transfection-Reagent DNA 6-well plate 1 6:1 2003. Nat. Cell Biol. 5: 711–9
BEAS-2B-19
FuGENE-6-Transfection-Reagent DNA 100 mm dish 0.01 not reported 2007. RNA 13: 1775-86
BHK-1
FuGENE-HD-Transfection-Reagent DNA 100mm dish 19 not reported 2011. Clin. Vaccine Immunol. 18: 268-79
BPAECS
FuGENE-6-Transfection-Reagent DNA 100 mm dish 5-10 not reported 2000. FASEB J. 14: 2486-94
C2C12
FuGENE-6-Transfection-Reagent DNA 35 mm dish 5 1.2:1 2003. Hum. Mol. Genet. 12: 189-203
C2C12
FuGENE-6-Transfection-Reagent siRNA not reported not reported not reported 2003. Neuron 40: 703-17
C2C12
FuGENE-HD-Transfection-Reagent DNA not reported not reported 4:2 2011. J. Biol. Chem. 286: 40296–306
Calu-6
FuGENE-6-Transfection-Reagent DNA 12-well plate 0.23 4:1 1998. J. Biol. Chem. 273: 25292-300
CHO
FuGENE-6-Transfection-Reagent DNA 100 mm dish 4-5 2.5:1 2002. Am. J. Physiol. Cell Physiol. 283: C850–65
CHO
FuGENE-6-Transfection-Reagent DNA 24-well plate 0.275 3:1 2003. Endocrinology 144: 4410–9
CHO
FuGENE-6-Transfection-Reagent DNA 24-well plate 0.25 2:1 2005. J. Clin. Endocrinol. Metab. 90: 507–515
CHO
FuGENE-6-Transfection-Reagent DNA 6-well plate 1 2.5:1 2002. Am. J. Physiol. Cell Physiol. 283: C850–65
CHO
FuGENE-6-Transfection-Reagent DNA not reported not reported 3:1 2002. Infect. Immun. 70: 4571-80
CHO-K1
FuGENE-6-Transfection-Reagent DNA 100mm dish 6 3:1 2010. J. Biol. Chem. 285: 19288–98
CHO-K1
FuGENE-6-Transfection-Reagent DNA 60mm dish 1.03 6:1.03 2010. J. Biol. Chem. 285: 19288–98