Coincidence Reporter Vectors

Part Numbers: N1461, N1471, N1481, N1491

E6751_pGL4-20-luc2-Puro-Vector_3
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同じmRNA転写物からホタルルシフェラーゼとNanoLuc®の両方を発現

  • ホタルとNanoLuc® ルシフェラーゼは化合物に対して異なる相互作用プロファイルを有しているため、片方のレポーターを単独使用するよりも偽陽性の同定が容易
  • 2つの異なるレポーターにより偽陽性比が低減し、真の生物学的陽性の同定率が向上
  • luc2 と NlucPはより明るいレポーターの組み合わせなので、低いコピー数、プレートのスケールアップに対応し、他のレポーターに比べシグナル/バックグラウンド比が得られる

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選択製品のカタログ番号: N1461

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Coincidence Reporter Vectors
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Coincidence reporter assay principle.

pNLCoI reporter vectors express the firefly luciferase and the destabilized NanoLuc® luciferase reporters on the same mRNA transcript. The two reporters are separated by a short P2A peptide, which causes ribosome skipping and the generation of two soluble, unfused forms of luciferase with distinct inhibitor profiles.

1種類の転写物から2種類のレポーター

ルシフェラーゼレポーターを用いたアッセイは、化合物のハイスループットスクリーニングを行う上で有用で強力な手法です。しかし、ルシフェラーゼと化合物が直接相互作用して偽陽性が生じると余分な労力をかけなければいけない場合があります。pNLCoI Vectors は次世代のコインシデント(一致)ベクターシステムで、同じmRNA転写物からホタルルシフェラーゼ(luc2)とPEST不安定化ドメインが融合したNanoLuc®(NlucP)の両方を発現します。2つのルシフェラーゼが当量発現するのは豚テシオウイルス-1 (Porcine teschovirus)由来のP2A配列を用いることにより、リボソームスキップ機構が促進され、2つの非融合ルシフェラーゼ酵素が得られるためです。これにより2つのルシフェラーゼに対する化合物との相互作用プロファイルが得られます。化合物のハイスループットスクリーニングで使用すれば、どちらか片方のルシフェラーゼと化合物との直接相互作用による偽陽性が、両方のルシフェラーゼで同様の反応を示す真の陽性と区別することができるため、余分なフォローアップスクリーニングに伴う労力を低減することができます。

pNLCoI Vectorは、同一サンプルよりホタルとNanoLuc® ルシフェラーゼを連続測定できるNano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay System ともに使用するためにデザインされています。この試薬を用いることにより両ルシフェラーゼの発光半減期が約2時間の安定な長時間発光シグナルを生じるため、サンプルのバッチ処理や 96-, 384- または 1,536-ウェルプレートフォーマットでのアッセイ/スクリーニングに使用することができます。この試薬のホタルルシフェラーゼの優れたクエンチング効果とNanoLuc® ルシフェラーゼの高レベルの発光により両レポーターの検出感度を最大限に引き上げることができます。


  • 信頼性の向上と時間の節約:2つの異なるレポーターにより偽陽性比が低減し、真の生物学的陽性の同定率が向上し、偽陽性に対するフォローに無駄な時間を費やす必要がありません。
  • 頑健性と感度を備えたレポーターペアを採用:luc2とNlucPはより明るいレポーターの組み合わせなので、低いコピー数、プレートのスケールアップに対応し、他のレポーターに比べシグナル/バックグラウンド比が得られます。
  • 効率的な偽陽性の同定:ホタルおよびNanoLuc® ルシフェラーゼは化合物に対して異なる相互作用プロファイルを有しているため、片方のレポーターを単独使用するよりも偽陽性の同定が容易です。
  • シンプルな検出フォーマット:簡便な ”添加 - 測定 - 添加 - 測定” ホモジニアスフォーマットは自動化やHTSに理想的   

【ライセンス】NanoLuc
12456MA-W
12457MA-W
12458MA-W
12459MA-W

pNLCoI1[luc2-P2A-NlucP/Hygro] Vector GenBank® Accession Number KM359771 and vector sequence text file.

pNLCoI2[luc2-P2A-NlucP/minP/Hygro] Vector GenBank® Accession Number KM359772 and vector sequence text file.

pNLCoI3[luc2-P2A-NlucP/CMV/Hygro] Vector GenBank® Accession Number KM359773 and vector sequence text file

pNLCol4[luc2-P2A-NlucP/PGK/Hygro] Vector  GenBank® Accession Number KM376434 and vector sequence text file.

製品仕様

You are viewing: N1461 Change Configuration

選択製品の構成品内容

Item Part # Size

pNLCoI1[luc2-P2A-NlucP/Hygro] Vector

N146A 1 × 20μg

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

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パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to, (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequences of the luciferase genes except that researcher may (1) create fused gene sequences provided that the coding sequences of the resulting luciferase genes have no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequences; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferases. No other use of this product or derivatives is authorized without the prior express written consent of Promega.

In addition, researcher must:
(1a)  use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researcher must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.

Researcher may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

製品仕様

You are viewing: N1471 Change Configuration

選択製品の構成品内容

Item Part # Size

pNLCoI2[luc2-P2A-NlucP/minP/Hygro] Vector

N147A 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to, (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequences of the luciferase genes except that researcher may (1) create fused gene sequences provided that the coding sequences of the resulting luciferase genes have no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequences; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferases. No other use of this product or derivatives is authorized without the prior express written consent of Promega.

In addition, researcher must:
(1a)  use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researcher must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.

Researcher may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

製品仕様

You are viewing: N1481 Change Configuration

選択製品の構成品内容

Item Part # Size

pNLCoI3[luc2-P2A-NlucP/CMV/Hygro] Vector

N148A 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to, (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequences of the luciferase genes except that researcher may (1) create fused gene sequences provided that the coding sequences of the resulting luciferase genes have no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequences; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferases. No other use of this product or derivatives is authorized without the prior express written consent of Promega.

In addition, researcher must:
(1a)  use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researcher must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.

Researcher may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

製品仕様

You are viewing: N1491 Change Configuration

選択製品の構成品内容

Item Part # Size

pNLCoI4[luc2-P2A-NlucP/PGK/Hygro] Vector

N149A 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to, (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequences of the luciferase genes except that researcher may (1) create fused gene sequences provided that the coding sequences of the resulting luciferase genes have no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequences; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferases. No other use of this product or derivatives is authorized without the prior express written consent of Promega.

In addition, researcher must:
(1a)  use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researcher must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.

Researcher may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

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