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Western Blue® Stabilized Substrate for Alkaline Phosphatase

Part Numbers: S3841

S3841_Western-Blue--Stabilized-Sub-for-AlkPhos_3
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  • Ready-To-Use の便利な試薬（試薬の希釈や混和が不要）
  • 市販の BCIP/NBT 試薬よりも高感度
  • 室温で1年間 安定

Size

選択製品のカタログ番号: S3841

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This product is discontinued
This product is available under our Early Access program - Learn More
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Western Blue® Stabilized Substrate for Alkaline Phosphatase
100ml
Your price:
ウエスタンブロットやイムノスクリーニングに使用できる安定なアルカリホスファターゼの基質です。Western Blue®Substrateは希釈せずにそのまま使用します。この基質は5-bromo-4-chloro-3-indolyl-1-phosphate(BCIP)とnitro blue tetrazolium(NBT)を専用の安定化バッファーに溶解しています。アルカリホスファターゼにより、退色しにくい黒紫色のスポットがメンブレン上に発色されます。Western Blue®Stabilized Substrateは、調製が必要なBCIP/NBT試薬と同等の検出感度を持ち、室温で1年間は安定です。

  • 便利: 希釈・混和せずに、そのまま使用可能
  • 安定: 室温で1年間安定

  • ウェスタンブロッティング
  • ドットブロッティング

  1. Cedeno-Laurent, F. et al. (2012) Galectin-1 triggers an immunoregulatory signature in Th cells functionally defined by IL-10 expression. J. Immunol. 188, 3127–37.
  2. Petrova, N.S. et al. (2012) Carrier-free cellular uptake and the gene-silencing activity of the lipophilic siRNAs is strongly affected by the length of the linker between siRNA and lipophilic group. Nucleic Acids Res. 40, 2330–44.
  3. To, W.S. and Midwood, K.S. (2011) Identification of novel and distinct binding sites within Tenascin-C for soluble and fibrillar fibronectin. J. Biol. Chem. 286, 14881–91.
Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells.

The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of β-actin (ng). Primary antibody (monoclonal) used at 1:5,000; Anti-Mouse IgG, AP Conjugate secondary antibody (Cat.# S3721) used at 1:2,500; blot imaged with Western® Blue Stabilized Substrate for Alkaline Phosphatase (Cat.# S3841).

製品仕様

SDS

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分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

技術資料

サイテーション

その他

類似製品

AttoPhos® AP Fluorescent Substrate System

高感度なアルカリフォスファターゼ (AP) 蛍光基質

S1000, S1001

BCIP/NBT Color Development Substrate

アルカリホスファターゼ (AP) 活性の発色検出用試薬

S3771

Blocking Agents

AP 標識 2次抗体による免疫ブロッティングで使用。Tween® 20 は抗体プローブとの非特異的な結合を解離

W3841, W3831

TMB Stabilized Substrate for Horseradish Peroxidase

ホースラディッシュペルオキシダーゼ (HRP) 検出用の安定な発色試薬

W4121

関連製品

Anti-Mouse IgG (H+L), AP Conjugate

全てのマウス IgG サブクラスの重鎖および軽鎖と反応する AP 標識 2次抗体

S3721

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