pNLF1 NanoLuc® Protein Fusion Vectors

Part Numbers: N1351, N1361, N1371

N1311_pFN31A-Nluc-CMV-Hygro-Flexi-Vector_3
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目的タンパク質のN末端かC末端にNluc全長を、あるいはN末端に分泌型Nlucを融合する

  • マルチクローニングサイトを使用した従来通りの方法でクローニングが可能
  • NanoLuc®レポーターは非常に明るいため、内在性レベルでのNanoLuc® 融合タンパク質発現が可能
  • 安定発現株作製が可能な選択マーカーを導入(ハイグロマイシン)

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選択製品のカタログ番号: N1351

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pNLF1 NanoLuc® Protein Fusion Vectors
N-terminal Nluc/20µg
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NanoLuc® (Nluc)ルシフェラーゼは分子量が小さく(19.1kDa)、非常に明るいため(ホタル: Photinus pyralis、ウミシイタケ: Renilla reniformisのルシフェラーゼの約100倍)、融合タンパク質として理想的です。NanoLuc®との融合タンパク質はタンパク質の安定性をモニタリングするレポーターとして、発光細胞イメージング(BLI)のプローブとして、あるいはタンパク質間やタンパク質:低分子間の相互作用を生物発光共鳴エネルギー移動(BRET)で検出する際のドナーとしての使用など、様々なアプリケーションに利用することが可能です。

NanoLuc®タンパク質融合ベクターで目的タンパク質とNanoLuc®を簡単に融合させることができます。また、クローニング方法の好みによって2種類のベクターから選べます。
 • pNLF Vectorシリーズ:目的タンパク質のN末端かC末端にNluc全長を、あるいはN末端に分泌型Nlucを融合するためのベクターです。マルチクローニングサイトを使用した従来通りの方法でクローニングが可能です。
 • pF Vectorシリーズ:Flexi® Vector Cloning Systemを利用して、目的タンパク質のN末端かC末端にNluc全長を融合するためのベクターです。Flexi® Vector Cloning Systemはレアカッター制限酵素であるSgfIとPmeIを使用し、インサートの方向性、読み枠を維持したまま効率よくクローニングやFlexi®ベクター間の移変えを行えるシステムです。


  • 発現量の測定が簡便:Nano-Glo® Luciferase Assay System 試薬を添加するとNanoLuc® 融合タンパク質から発光シグナルが得られ、細胞内タンパク質レベルを測定可能
  • より生物学的妥当性のある結果を:NanoLuc®レポーターは非常に明るいため、内在性レベルでのNanoLuc® 融合タンパク質発現が可能で、過剰発現によるアーティファクトを回避
  • 細胞内タンパク質の可視化:細胞毒性の原因となる励起光の照射は不要
  • BRETに好適:青にシフトした明るいNanoLuc®の蛍光スペクトラムにより、より良いシグナル・バックグラウンド比とダイナミックレンジを実現
  • 選べるクローニング方法:従来のクローニング法とFlexi® Cloning systemを選択可能
  • 安定発現株作製可能:全てのベクターに安定発現株作製用の選択マーカーを導入

  • 化合物スクリーニング
  • タンパク質間相互作用解析
  • タンパク質:低分子相互作用解析
  • 生物発光イメージング
  • タンパク質安定性

【ライセンス】NanoLuc

【Q&A】Reporter_1.


  1. Hall, M.P. (2012) Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chemical Biology 7(11), 1848–57.
12007MA-W

pNLF1-N [CMV/Hygro] Vector GenBank® Accession Number KF811457 and vector sequence text file.

12008MA-W

pNLF1-C [CMV/Hygro] Vector GenBank® Accession Number KF811458 and vector sequence text file.

12009MA-W

pNLF1-secN [CMV/Hygro] Vector GenBank® Accession Number KF811459 and vector sequence text file.

製品仕様

You are viewing: N1351 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration

pNLF1-N [CMV/Hygro] Vector

N135A 1 × 20μg 1μg/μl

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researchers may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researcher may (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use of this product or derivatives is authorized without the prior express written consent of Promega.

In addition, researchers must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.

Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

製品仕様

You are viewing: N1361 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration

pNLF1-C [CMV/Hygro] Vector

N136A 1 × 20μg 1μg/μl

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researchers may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researcher may (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use of this product or derivatives is authorized without the prior express written consent of Promega.

In addition, researchers must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.

Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

製品仕様

You are viewing: N1371 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration

pNLF1-secN [CMV/Hygro] Vector

N137A 1 × 20μg 1μg/μl

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researchers may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researcher may (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use of this product or derivatives is authorized without the prior express written consent of Promega.

In addition, researchers must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.

Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

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