Nano-Glo® Luciferase Assay System

Part Numbers: N1110, N1120, N1130, N1150

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NanoLuc® Luciferaseのための、添加・混和・測定アッセイ

  • 明るいNanoLuc®レポーターなら、より感度が求められる難しい実験用途に使用可能
  • 添加して測定するだけのシンプルな操作性によりHTSへの変更が容易
  • 発光時間が長いので、バッチ操作あるいは連続操作に最適化

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選択製品のカタログ番号: N1110

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Nano-Glo® Luciferase Assay System
10ml
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NanoLuc® ルシフェラーゼの高感度検出を可能にする、明るく持続性の高いシグナル

Nano-Glo® Luciferase Assay Reagent は NanoLuc® Luciferase.の検出用のアッセイシステムです。Nano-Glo® Luciferase Assay Systemは1種類の試薬を加えるだけのシンプルな操作性を提供し、一般的な培地で使用した場合、半減期約2時間におよぶグロータイプの発光シグナルが得られます。試薬は Nano-Glo® Luciferase Assay SubstrateとNano-Glo® Luciferase Assay Bufferを混和するだけで調製でき、試薬には溶解バッファーが含まれており、NanoLuc® luciferaseを発現する細胞あるいはルシフェラーゼが分泌された培地に直接使用することができます。

Nano-Glo® Luciferase Assay Reagent はNanoLuc® Luciferase検出用の専用試薬です。NanoLuc® Luciferaseの詳細については NanoLuc®ルシフェラーゼをご参照ください。

  • ハイエンドなレポータシステム:発光量の高いNanoLuc®レポーターはより感度が求められる難しい実験用途に使用可能
  • 最適化が容易:添加して測定するだけのシンプルな操作性によりHTSへの変更が容易
  • 測定精度の改善:溶解操作と試薬の添加操作を分ける必要はありません
  • より明るく長い発光時間:バッチ操作あるいは連続操作に最適化
  • 自己発光の低減:低いバックグラウンドを実現する試薬組成により感度が向上

【ライセンス】NanoGlo

【簡易マニュアル】日本語
※日本語マニュアルは英語版を翻訳したものですが、常に更新されるものではありません。最新のマニュアルについては英語版(”Product Resources”タブ → “Protocols”)をご覧ください。

【Q&A】Nano-Glo Luciferase Assay System

A comparison of the sensitivity of NanoLuc®, firefly and Renilla luciferase assays.
NanoLuc® ルシフェラーゼは、ホタルおよびRenillaルシフェラーゼアッセイと比べて100倍の感度を示す。

アッセイの特長

  • 高度なレポーターシステム:高輝度の NanoLuc® レポーターにより、検出が困難なアプリケーションでも高感度な測定が可能
  • 持続的で明るい発光出力は、バッチ処理や連続処理に最適
  • アッセイ最適化が簡単:試薬を加えるだけのシンプルな操作でアッセイが可能。細胞溶解や試薬注入といった別工程は不要で、スケールアップも容易。
  • 低バックグラウンド設計で感度向上

カスタム仕様も可能です。詳細は www.promega.com/custom/ をご覧ください。
バルク購入に関しては弊社までお問い合わせください。

Signal decay kinetics for Nano-Glo® Luciferase Assay Reagent.
Nano-Glo® Luciferase Assay Reagentののシグナル持続性

製品仕様

You are viewing: N1110 Change Configuration

選択製品の構成品内容

Item Part # Size
Nano-Glo® Luciferase Assay Buffer N112A 1 × 10ml
Nano-Glo® Luciferase Assay Substrate N113A 1 × 200μl

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

製品仕様

You are viewing: N1120 Change Configuration

選択製品の構成品内容

Item Part # Size
Nano-Glo® Luciferase Assay Buffer N112B 1 × 100ml
Nano-Glo® Luciferase Assay Substrate N113B 2 × 1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

製品仕様

You are viewing: N1130 Change Configuration

選択製品の構成品内容

Item Part # Size
Nano-Glo® Luciferase Assay Buffer N112A 10 × 10ml
Nano-Glo® Luciferase Assay Substrate N113A 10 × 200μl

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

製品仕様

You are viewing: N1150 Change Configuration

選択製品の構成品内容

Item Part # Size
Nano-Glo® Luciferase Assay Buffer N112B 10 × 100ml
Nano-Glo® Luciferase Assay Substrate N113C 5 × 4ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

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