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Long-Range PCR: GoTaq® Long PCR Master Mix

Part Numbers: M4021

M4021_GoTaq--Long-PCR-Master-Mix_3
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PCR Satisfaction Guaranteed

効率的な長鎖 DNA 増幅用 ホットスタートマスターミックス

  • 伸長効率と忠実性を高めた耐熱性 DNA ポリメラーゼの特殊ブレンド
  • 便利な室温セットアップが可能なホットスタートマスターミックス
  • 収量、感度、特異性 改良のために最適化

Size

選択製品のカタログ番号: M4021

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Long-Range PCR: GoTaq® Long PCR Master Mix
100 reactions
Your price:

Higher Yields with Hot-Start Convenience for Longer Amplicons

GoTaq® Long PCR Master Mixはパフォーマンスに優れる GoTaq® Hot Start Polymeraseと独自の耐熱性ハイフィデリティーポリメラーゼを組み合わせたマスターミックスです。この最適化された酵素ミクスチャーはラムダDNAより最長40Kb、ヒトゲノムDNAより最長30kbまでを効率的に増幅することができます。伸長効率の高いポリメラーゼとDNAミスマッチを修復するハイフィデリティー酵素が共役することでDNAの伸長反応を持続させることができるため、長いDNA増幅産物を得ることができます。最適化されたGoTaq® Long PCR Master Mixは室温で簡便にPCR反応をセットアップでき、長いDNA増幅産物を確実、効率的に得るために調整されています。


  • 便利：簡便な操作性とシンプルなセットアップが可能なホットスタートマスターミックス
  • 強化：最適化されたコンポネントにより収量、感度、特異性が向上
  • ロングPCR：伸長性と正確性に優れる熱安定DNAポリメラーゼのブレンド. 30kb gDNAまでのロングPCRに対応
  • 信頼性：反応コントロールあるいは鋳型の質をテストするためのコントロールプライマーペアおよびヒトゲノム鋳型DNAが添付
  • 効率的：遺伝子クローニング、変異分析、DNAシークエンシングに最適
  • 感度向上：2ngのテンプレートから特異的な増幅

【Ｑ＆Ａ】GoTaqについて

pcr-guaranteegray-circlePerformance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

10214TA_350px_longpcr
GoTaq® Long PCR Master Mix (lane P) outperforms other leading long PCR products (lanes T, N, L and S) for amplification of a 17.5kb human β-globin target sequence. P: GoTaq® Long PCR Master Mix; T: TaKaRa LA Taq™ Hot Start Version; N: New England BioLabs: LongAmp® Taq 2X Master Mix; L: Life Technologies: GeneAmp® XL PCR Kit; S: Sigma: JumpStart™ AccuTaq™ LA DNA Polymerase Mix; M: Lambda DNA/HindIII Markers; G171

GoTaq® Long PCR Master Mix Advantages:

  • Easy Setup: Handle reactions at room temperature.
  • Enhanced Yields: Optimized components maximize results.
  • Improved Processivity: Enzyme blend provides robust amplification.
  • Assured Confidence: Includes control primer pair and gDNA template.
  • Improved Sensitivity: Amplify as little as 2ng with high specificity

Publications Using GoTaq® Long PCR Master Mix

 

Application Target Size Citation
Sanger Sequencing Virus ~4kb Schleiss, M. et al. (2014) Molecular and biological characterization of a new isolate of guinea pig cytomegalovirus. Viruses 6, 448–476.
Illumina & Pacific Biosciences Sequencing for HLA Typing HLA Genes 4.4–12.4kb Albrecht, V. et al. (2017) Dual redundant sequencing strategy: Full-length gene characterisation of 1056 novel and confirmatory HLA alleles. HLA 90, 79–87.
Oxford Nanopore Sequencing for HLA Typing HLA Genes ~4.5kb Liu, C. et al. (2018) Accurate typing of human leukocyte antigen class I genes by Oxford Nanopore sequencing. J. Mol. Diagn. 20, 428–435.
Cloning & Sanger Sequencing Complete Plant ORF 7.8kb Steuernagel, B. et al. (2016) Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture. Nat. Biotechnol. 334, 652-655.
 

プロトコール

Complete Protocol

製品仕様

選択製品の構成品内容

Item Part # Size Available Separately

Nuclease-Free Water

 P119E 1 × 13ml View Product

Human Genomic DNA

 G304B 1 × 30μg

GoTaq® Long PCR Master Mix, 2X

 M402A 2 × 1.25ml

Long PCR Control Primer Pair

 M412A 1 × 50μl

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

技術資料

技術記事

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