HiBiT CMV-neo Flexi® Vectors
Vectors for Tagging Proteins with HiBiT for Luminescent Expression Analysis
- Add an N- or C-terminal HiBiT tag to your protein of interest
- Generate an N-terminal HiBiT-tagged transmembrane or secreted protein
- Use with the Flexi® Cloning System to transfer ORF insert
Vectors
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| N2391 | pFC37K HiBiT CMV-neo Flexi® Vector | View Specifications | 20μg | Please Enquire | |||
| N2401 | pFN38K HiBiT CMV-neo Flexi® Vector | View Specifications | 20μg | Please Enquire | |||
| N2411 | pFN39K secHiBiT CMV-neo Flexi® Vector | View Specifications | 20μg | Please Enquire |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
3種類の HiBiT CMV-neo Flexi® Vector は標的タンパク質の N末端または C末端に HiBiT ペプチドタグを付加するために、Flexi® Cloning System (Cat.# C8640) を用いて簡便で効率よく目的遺伝子をクローニングするために設計された発現ベクターです。ベクターは安定または一過性の遺伝子発現に使用でき、バクテリアでのセレクション用のカナマイシン、哺乳動物でのセレクション用のネオマイシンをコードしています。
pFC37K HiBiT CMV-neo Flexi® Vector は目的遺伝子の C末端に HiBiTタグの配列を付加
pFN38K HiBiT CMV-neo Flexi® Vector は目的遺伝子の N末端に HiBiTタグの配列を付加
pFN39K secHiBiT CMV-neo Flexi® Vector は目的遺伝子の N末端に HiBiTタグの配列を付加
本ベクターは細胞表面での発現あるいは分泌タンパク質のために HiBiT タグのN末端側へ IL-6 分泌シグナルペプチドをコードしたベクターです。
備考: HiBiTタグタンパク質を効率的に細胞表面で発現させるために、目的遺伝子に本来コードされた分泌シグナルを除去することを推奨しています。
備考:
1. HiBiT付きタンパク質の発現は、HiBiTタグと目的遺伝子との間で適切な読み枠が維持されている必要があります。
2. HiBiTペプチド配列は参照用です。HiBiT タグを合成する権利等については使用条件についてご確認ください。
【Q&A】 HiBiT
Flexi® Vector System について
Flexi® Vector System はタンパク質コード配列のディレクショナルクローニング (directional cloning) を行うシンプルで強力なシステムです。2種類のレアカッター制限酵素 SgfI および PmeI による消化をベースにしており、様々な Flexi® Vector 間でのタンパク質コード領域を迅速、効率的、高い忠実性で移換えられるので、シークエンスの確認も不要です。組換えクローンをそのまま使用できるため、バックグラウンドコロニーよりスクリーニングする時間を節約することができます。 また、Flexi® system の汎用性により様々な発現系や融合タグより選ぶことができます。ダイレクトな組換えは２つのN末端タグベクター間またはN末端ベクターからC末端ベクターで行うことができます。
備考：サブクローニングを行う上で、遺伝子が挿入されていないバックグラウンドコロニーを低減するためにFlexi® Vector にはバーナーゼ致死遺伝子が含まれています。Flexi® Vector Cloning System ではバーナーゼ遺伝子と目的遺伝子が置き換わります。購入した空の Flexi® ベクターは標準的な実験で用いられる大腸菌株では増やすことはできません。
すぐに使用できる HaloTag® Flexi® ORF クローン
プロメガの Flexi® ORF Clone は ヒト ORF 20,000 クローン、マウス ORF 15,000 クローン を揃えたクローンコレクションで 、Flexi® HiBiT ベクターへも容易にORFを移換えることができます。詳細についてはORFこちらをご覧ください。
pFC37K HiBiT CMV-neo Flexi® Vector sequence text file.
pFN38K HiBiT CMV-neo Flexi® Vector sequence text file.
pFN39K secHiBiT CMV-neo Flexi® Vector sequence text file.
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
pFC37K HiBiT CMV-neo Flexi® Vector |
N239A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
No transfer of this product is allowed. Researchers may use this product in their own research and they may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to recipients and recipients agree to be bound by the terms of this label license.
Researchers shall have no right to modify or otherwise create variations of the nucleotide sequences of the subunits of NanoBiT® Technology, except that researchers may create fused gene sequences at the termini of the subunits of NanoBiT® Technology. No other use of this product or its derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must either:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives, or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must either:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives, or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researcher has no right to modify, derivatize, genetically engineer or otherwise create variations of the NanoBiT® Control Vectors, except that researcher may propagate and store NanoBiT® Control Vectors for use in NanoBiT® assays. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 9,797,890 and 10,648,971; Japanese Pat. Nos. 7270842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
pFN38K HiBiT CMV-neo Flexi® Vector |
N240A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
No transfer of this product is allowed. Researchers may use this product in their own research and they may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to recipients and recipients agree to be bound by the terms of this label license.
Researchers shall have no right to modify or otherwise create variations of the nucleotide sequences of the subunits of NanoBiT® Technology, except that researchers may create fused gene sequences at the termini of the subunits of NanoBiT® Technology. No other use of this product or its derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must either:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives, or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must either:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives, or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researcher has no right to modify, derivatize, genetically engineer or otherwise create variations of the NanoBiT® Control Vectors, except that researcher may propagate and store NanoBiT® Control Vectors for use in NanoBiT® assays. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 9,797,890 and 10,648,971; Japanese Pat. Nos. 7270842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
pFN39K secHiBiT CMV-neo Flexi® Vector |
N241A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
No transfer of this product is allowed. Researchers may use this product in their own research and they may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to recipients and recipients agree to be bound by the terms of this label license.
Researchers shall have no right to modify or otherwise create variations of the nucleotide sequences of the subunits of NanoBiT® Technology, except that researchers may create fused gene sequences at the termini of the subunits of NanoBiT® Technology. No other use of this product or its derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must either:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives, or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must either:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives, or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researcher has no right to modify, derivatize, genetically engineer or otherwise create variations of the NanoBiT® Control Vectors, except that researcher may propagate and store NanoBiT® Control Vectors for use in NanoBiT® assays. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 9,797,890 and 10,648,971; Japanese Pat. Nos. 7270842 and 7532562; and other patents and patents pending.
Resources
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