tigit-cd155-blockade-bioassay

Part Numbers: J2201, J2205, J2092, J2051, J1921

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免疫チェックポイントシグナル遮断のためにデザインされたバイオロジックスの力価および安定性測定に

  • ICH ガイドラインに認証済み
  • 96- および 384-ウェルプレートフォーマット対応
  • 初代培養細胞は不要

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選択製品のカタログ番号: J2201

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tigit-cd155-blockade-bioassay
Thaw-and-Use Kits/1X each component
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免疫チェックポイントシグナル遮断するバイオロジッツクスの力価測定に

TIGIT (WUCAM または Vstm3 としても知られる) はリンパ球で発現する免疫チェックポイントタンパク質です。TIGIT は エフェクター CD4+ および CD8+ T 細胞、制御性 T 細胞、NK細胞で高レべルな発現が認められます。TIGIT はリンパ球の活性化を阻害するいくつかの作用機序を有しています。第一にTIGITは共刺激受容体 CD226 の作用を阻害するカウンターパートです。TIGIT がリンパ球表面に存在するとCD155と優勢的に結合し、CD226のシグナルを遮断します。第二に TIGIT はシス型にCD226と相互作用し、CD226 のホモ二量体化を阻害します。第3にTIGIT の細胞質尾部には免疫受容体チロシンベースの阻害モチーフ (ITIM) を含み、潜在的な阻害シグナルを誘導します。

TIGIT/CD155 遮断バイオアッセイはTIGIT/CD155 相互作用をブロックするためにデザインされたバイオロジックスの作用機序を反映しています。

アッセイの構成

本アッセイは遺伝子改変された2種類の細胞株で構成されています:

TIGIT Effector Cells : ヒト TIGIT と TCR 活性化および CD226 共刺激の両方に応答するネイティブプロモーターより駆動するルシフェラーゼレポーター を発現する Jurkat T 細胞

CD155 aAPC/CHO-K1 Cells 
: ヒトCD155 とともに抗原非依存的にTCR 複合体を活性化するために遺伝子改変された細胞表面タンパク質を発現する CHO-K1 細胞
本アッセイは遺伝子改変された2種類の細胞株で構成されています:

ワークフロー

従来法 (プライマリー細胞を使用)

Standard bioassay workflow with cultured cells
発光バイオアッセイ法(試薬として細胞も供給)
Improved bioassay workflow with thaw-and-use cells

標準化された試薬で結果が向上

  • "溶かして" & "使う" だけの(Thaw-and-use)細胞フォーマットなら従来法に比べてバラつきが低減されるだけでなく時間と労力を著しく節約
  • 標準化されたフォーマットとして全ての必要な試薬(細胞も!)がオールインワンになったキット
  • ハイスループットなワークフローにも簡単に適合

アッセイの原理

TIGIT/CD155 Blockade Bioassay は従来のアッセイ法の限界を超えて、TIGITを標的とした抗体やその他のバイオロジックスの力価や安定性の測定に使用することができます。

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Representation of the TIGIT/CD155 Blockade Bioassay. The bioassay consists of two genetically engineered cell lines, TIGIT Effector Cells and CD155 aAPC/CHO-K1 Cells. Panel A. When co-cultured, TIGIT inhibits CD226 pathway-activated luminescence. The addition of anti-TIGIT antibody blocks the TIGIT/CD155 interaction, thereby re-establishing CD226 pathway-activated luminescence, which can be detected in a dose-dependent manner by addition of BIo-Glo™ Reagent and quantitation with a luminometer. Panel B. When co-cultured with non-TIGIT-expressing Effector Cells, CD155 induces luminescence by activation of the CD226 pathway.

アッセイパフォーマンスデータ

正確度 (accuracy) と 精度 (precision) はバイオアッセイやバイオロジックス開発において非常に重要です。TIGIT/CD155 Blockade Bioassay は正確度と 精度を兼ね備えたアッセイです(正確度とは標的または参照値に対してどれほど近かったかという度合いであり、精度は再現性の度合いです)。本アッセイは正確度 と 精度 のためのICHガイドラインに従い開発、事前承認されており、抗体医薬の研究開発に使用するバイオアッセイの様々なアプリケーションに必要とされるパフォーマンス特性を備えています。

32454752-data-target-combined

The TIGIT/CD155 Blockade Bioassay Shows Precision, Accuracy and Linearity.

Parameter Results
Accuracy % Expected Relative Potency % Recovery
50 101.8
70 102.8
140 101.0
200 98.5
Repeatability (% CV) 100% (Reference) 11.0
Intermediate Precision (% CV)   9.7
Linearity (r2)   0.999
Linearity (y = mx + b)   y = 0.974x + 3.33
A 50–200% theoretical potency series of Control Ab, Anti-TIGIT, was analyzed in triplicate in three independent experiments performed on three days by two analysts. Bio-Glo™ Reagent was added and luminescence quantified using the GloMax® Discover Detection System. Data were analyzed and relative potencies calculated after parallelism determination using JMP® software. Data were generated using thaw-and-use cells.

The TIGIT/CD155 Blockade Bioassay reflects the mechanism of action (MOA) and specificity of biologics designed to block the TIGIT/CD155 interaction.
The TIGIT/CD155 Blockade Bioassay measures the activity of Control Ab, Anti-TIGIT.
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TIGIT Effector Cells were incubated with CD155 aAPC/CHO-K1 Cells in the presence of a serial titration of Control Ab, Anti-TIGIT (Cat.# J2051), anti-PD-1 (nivolumab), anti-CTLA-4 (ipilimumab) or anti-PD-L1 blocking Abs as indicated. 
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TIGIT Effector Cells were plated overnight. The following day, a titration fo Control Ab, Anti-TIGIT, was added followed by CD155 aAPC/CHO-K1 Cells.

キットのフォーマットと関連製品

  • 1X および 5X サイズより選択可能
  • Control Ab, Anti-TIGIT の別売品

製品仕様

You are viewing: J2201 Change Configuration

選択製品の構成品内容

Item Part # Size

TIGIT Effector Cells

J169A 1 × 0.5ml

CD155 aAPC/CHO-K1 Cells

J180A 1 × 0.5ml

Fetal Bovine Serum

J121A 1 × 4ml

RPMI 1640 Medium

G708A 1 × 36ml

Bio-Glo™ Luciferase Assay Buffer

G719A 2 × 10ml

Bio-Glo™ Luciferase Assay Substrate

G720A 2 × 1 vial

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,077,244 and other patents.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

製品仕様

You are viewing: J2205 Change Configuration

選択製品の構成品内容

Item Part # Size

TIGIT Effector Cells

J169A 5 × 0.5ml

CD155 aAPC/CHO-K1 Cells

J180A 5 × 0.5ml

Fetal Bovine Serum

J121A 5 × 4ml

RPMI 1640 Medium

G708A 5 × 36ml

Bio-Glo™ Luciferase Assay Buffer

G719A 10 × 10ml

Bio-Glo™ Luciferase Assay Substrate

G720A 10 × 1 vial

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,077,244 and other patents.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

製品仕様

You are viewing: J2092 Change Configuration

選択製品の構成品内容

Item Part # Size

TIGIT Effector Cells (CPM)

J168A 2 × 1ml

CD155 aAPC/CHO-K1 Cells (CPM)

J179A 2 × 1ml

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells except that recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

製品仕様

You are viewing: J2051 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration

Control Ab, Anti-TIGIT

J205A 1 × 100μg 1mg/ml

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

CC

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

The transfer of this product is conditioned on the buyer using the purchased product for (i) research use, (ii) discovery, development and monitoring of biologic drugs and vaccines, (iii) quality assurance and quality control testing of biologic drugs and vaccines, and (iv) product release assays for biologic drugs and vaccines conducted by the buyer, including contract research or any fee for service research, and the buyer must not (1) use this product or its components for (1a) any use, indirectly (except as allowed in (iii) and (iv) above) or directly in manufacturing, or (1b) diagnostic, therapeutic or prophylactic purposes; and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research.

製品仕様

You are viewing: J1921 Change Configuration

選択製品の構成品内容

Item Part # Size

TCR/CD3 Effector Cells (IL-2)

J129A 1 × 0.8ml

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

XX

Please see the Protocol for detailed storage conditions.

パテントおよび免責条項

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

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