| Cell Line |
Transfection Reagent |
Type of Nucleic Acid Transfected |
Plate Format |
Amount of Nucleic Acid Used (µg) |
Ratio of Reagent to Nucleic Acid (µl:µg) |
Journal Citation |
10T1/2 |
FuGENE 6 Transfection Reagent |
DNA |
60 mm dish |
2 |
not reported |
2000. Mol. Cell. Biol. 20: 6600-11 |
Description of cell line: mouse embryonic fibroblast
Additional Relevant Information:
Type of Transfection:
|
 |
10T1/2 |
FuGENE 6 Transfection Reagent |
DNA |
96-well plate |
2.5 |
2:1 |
2003. J. Biol. Chem. 278: 17360 |
Description of cell line: mouse embryonic fibroblast
Additional Relevant Information: 10e5 cells plated
Type of Transfection:
|
|
HEK 293 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
3 |
3:1 |
2012. J. Virol. 86: 1193-1202 |
Description of cell line: adenovirus-transformed human embryonic kidney
Additional Relevant Information: HEK293FT cells, 30% confluent at time of transfection
Type of Transfection:
|
|
HEK 293T |
FuGENE 6 Transfection Reagent |
RNA |
12-well dish |
0.6 |
not reported |
2010. J. Virol. 84: 5656–9 |
Description of cell line: adenovirus-transformed human kidney cells, expressing SV40 large T antigen
Additional Relevant Information:
Type of Transfection:
|
|
NIH 3T3 |
FuGENE 6 Transfection Reagent |
DNA |
60 mm dish |
2.05 or 4.05 |
3:1 |
|
Description of cell line: mouse embryonic fibroblast
Additional Relevant Information: 5X10e5 cells plated, transfected at 50-60% confluency
Type of Transfection:
|
|
NIH 3T3 |
FuGENE 6 Transfection Reagent |
DNA |
not reported |
not reported |
6:4 |
2001. J. Biol. Chem. 276: 47038-45 |
Description of cell line: mouse embryonic fibroblast
Additional Relevant Information:
Type of Transfection:
|
|
3T3-L1 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
2 |
3:1 |
2000. J. Virol. 74: 5776–87 |
Description of cell line: mouse embryonic fibroblast
Additional Relevant Information: 2x10e5-4x10e5 cells plated 24h prior to transfection
Type of Transfection:
|
|
3T3-L1 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
1 |
not reported |
|
Description of cell line: mouse embryonic fibroblast
Additional Relevant Information: 1x10e6 cells/well plated
Type of Transfection:
|
|
10T1/2 |
FuGENE 6 Transfection Reagent |
DNA |
100 mm dish |
5 |
3:1 |
2005. J. Biol. Chem. 280: 8994–9004 |
Description of cell line: mouse embryonic fibroblast
Additional Relevant Information: 70% confluency
Type of Transfection:
|
|
A10 |
FuGENE 6 Transfection Reagent |
DNA |
12-well plate |
0.5 |
3:1 |
2000. J. Biol. Chem. 275: 23661–5 |
Description of cell line: rat embryonic thoracic aorta myoblast
Additional Relevant Information: Dulbecco's modified Eagle's medium + 1% antibiotic (no serum)
Type of Transfection:
|
|
A10 |
FuGENE 6 Transfection Reagent |
DNA |
35 mm dish |
2 |
3:2 |
2000. J. Biol. Chem. 275: 31162–70 |
Description of cell line: rat embryonic thoracic aorta myoblast
Additional Relevant Information: complete media; 1.4x10e5 cells plated
Type of Transfection:
|
|
A2870 |
FuGENE 6 Transfection Reagent |
DNA |
not reported |
1 |
3:1 |
2003. J. Biol. Chem. 278: 2249-55 |
Description of cell line: ovarian cancer cells
Additional Relevant Information:
Type of Transfection:
|
|
A375m2 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
1 |
6:1 |
2003. Nat. Cell Biol. 5: 711–9 |
Description of cell line: human melanoma
Additional Relevant Information: plated at 50% confluency 16hr prior to transfection
Type of Transfection:
|
|
A375P |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
1 |
6:1 |
2003. Nat. Cell Biol. 5: 711–9 |
Description of cell line: human melanoma
Additional Relevant Information: plated at 50% confluency 16hr prior to transfection
Type of Transfection:
|
|
A549 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
3 or 5 |
not reported |
2003. J. Biol. Chem. 278: 7445–7452 |
Description of cell line: human lung carcinoma
Additional Relevant Information:
Type of Transfection:
|
|
A549 |
FuGENE 6 Transfection Reagent |
DNA |
60 mm dish |
1.2 |
3:1 |
2008. J. Gen. Virol. 89: 1978–86 |
Description of cell line: human lung carcinoma
Additional Relevant Information:
Type of Transfection:
|
|
A549 |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
0.5 |
not reported |
2000. EMBO J. 19: 6759–69 |
Description of cell line: human lung carcinoma
Additional Relevant Information:
Type of Transfection:
|
|
A549 |
FuGENE 6 Transfection Reagent |
DNA |
96-well plate |
0.1-0.2 |
3:1 |
2005. Drug Metab. Dispos. 33: 1244–53 |
Description of cell line: human lung carcinoma
Additional Relevant Information:
Type of Transfection:
|
|
A549 |
FuGENE HD Transfection Reagent |
DNA |
6-well plate |
3 |
3:1 |
2012. J. Virol. 86: 8198–8209 |
Description of cell line: human lung carcinoma
Additional Relevant Information:
Type of Transfection:
|
|
AC29 |
FuGENE 6 Transfection Reagent |
DNA |
100 mm dish |
5 |
2:1 |
1998. J. Biol. Chem. 273: 26747–54 |
Description of cell line: CHO lacking ACAT activity
Additional Relevant Information:
Type of Transfection:
|
|