PowerPlex® 21 System

Part Numbers: DC8902, DC8942

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ヒト個人識別用マルチプレックスSTRシステム

  • 識別能力が向上し、米国、ヨーロッパ、アジアで共通して使用されているローカスを用いることでデータの共有の実現性も高まる
  • FTA®カードパンチの洗浄不要
  • PCRに必要な時間を2時間までに短縮

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選択製品のカタログ番号: DC8902

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PowerPlex® 21 System
200 reactions
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Fast Process and Flexible Protocols

【PowerPlex® 21 Systemは2016年5月より、新規蛍光色素により標識されたWEN ILS 500がインターナルレーンスタンダードとして付属します。詳細はこちらをご覧ください】

PowerPlex® 21 Systemは法医学分析、親子(親族)鑑定、研究用途におけるヒト個人識別のアプリケーションに使用するためのマルチプレックスSTRシステムです。システムは21ローカス(20種類のSTRローカスとアメロゲニン[D1S1656, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, Amelogenin, CSF1PO, FGA, Penta D, Penta E, TH01, TPOX, vWA])を同時に増幅、蛍光検出することができます。PowerPlex® 21 SystemはABI PRISM® 3100、3100-Avant Genetic Analyzers、Applied Biosystems 3130, 3130xl, 3500および3500xL Genetic Analyzersに適応します。

GeneMapper® ID および ID-X ソフトウエアによる遺伝子型の自動割り当てを行う際のPanelおよびbinファイルはこちらから入手可能


  • 21種類のマーカー: 識別能力が向上
  • 直接増幅が可能: FTA®カードパンチの洗浄不要、スワブやnonFTAカードでも簡易なプロトコール
  • 高インヒビター耐性: 困難なケースワークサンプルでもローカスドロップアウトや増幅の失敗が少ない
  • PCRは90分: PCRに必要な時間を短縮、研究室の生産性を向上

  • ヒト個人識別テスト
  • 法医学的ケースワーク
  • 犯罪者データベース
  • 親子(親族)鑑定
  • 細胞/組織サンプルの同定
  • 細胞認証
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A single-source template DNA (0.5ng) was amplified using the PowerPlex® 21 System and analyzed with an Applied Biosystems 3130 Genetic Analyzer using a 3kV, 5-second injection. Results were analyzed using GeneMapper® ID software, version 3.2.
ForensicGrade

プロメガの法医学関連製品は ISO 18385 に準拠して製造されています。これにより法医学的な解析を目的とする生体サンプルの採取、保存、分析を行うための製品にヒトDNAが混入するリスクが最小限に抑えられます。 詳細についてはこちら

Flexibility for Most Sample Types

The PowerPlex® 21 System is designed to work with a variety of sample types, including difficult casework samples and is compatible with direct amplification from FTA® card punches as well as pretreated swabs and Bode Buccal Collector™ device or S&S 903 punches.

Genomic DNA
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Amplification of 500pg of human genomic DNA using the recommended protocol. Amplified products were separated on an Applied Biosystems 3130 Genetic Analyzer (3kV, 5-second injection).
Blood on FTA® Card
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Direct amplification of one 1.2mm punch from blood on an FTA® card using recommended protocol. Amplified products were separated on Applied Biosystems 3130 Genetic Analyzer (3kV, 5-second injection). 
Buccal Samples on S&S 903
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A 1.2mm punch from buccal samples on S&S 903 was pre-treated with SwabSolution™ Reagent, then amplified using the recommended protocol. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection). 

Swab from Buccal Samples

10370TA-W
A swab from buccal samples was pre-treated with SwabSolution™ Reagent, then amplified using the recommended protocol. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection).

Rapid Processing Solutions for PowerPlex®

The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® Fusion, 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.

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製品仕様

You are viewing: DC8902 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration Available Separately

2800M Control DNA

DD710A 1 × 25μl 10ng/μl View Product

WEN Internal Lane Standard 500

DG500A 1 × 200μl View Product
PowerPlex® 21 Allelic Ladder Mix DG247B 1 × 100μl
PowerPlex® 21 5X Primer Pair Mix DK183B 1 × 1ml
PowerPlex® 21 5X Master Mix DP107A 1 × 1ml

Water, Amplification Grade

DW099A 5 × 1,250μl View Product

分析証明書

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使用制限

Not For Medical Diagnostic Use.

保存条件

BB

パテントおよび免責条項

U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.

TMR-ET, CXR-ET and WEN dyes are proprietary.

製品仕様

You are viewing: DC8942 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration Available Separately

2800M Control DNA

DD710A 4 × 25μl 10ng/μl View Product

WEN Internal Lane Standard 500

DG500A 4 × 200μl View Product
PowerPlex® 21 Allelic Ladder Mix DG247B 4 × 100μl
PowerPlex® 21 5X Primer Pair Mix DK183B 4 × 1ml
PowerPlex® 21 5X Master Mix DP107A 4 × 1ml

Water, Amplification Grade

DW0991 4 × 6,250μl

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

BB

パテントおよび免責条項

U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.

TMR-ET, CXR-ET and WEN dyes are proprietary.

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