Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

If you are located in the EEAEuropean Economic Area, the United Kingdom, or Switzerland, you can change your settings at any time by clicking Manage Cookies in the footer of our website.

Trypsin/Lys-C Mix, Mass Spec Grade

Part Numbers: V5071, V5072, V5073

V5072_Trypsin-3aLys-C-Mix--Mass-Spec-Grade_3
Protein Cleavage with Trypsin-Lys-C
V5072_Trypsin-3aLys-C-Mix--Mass-Spec-Grade_3
customize-this-small

Trypsin Gold, Mass Spec GradeとrLys-C, Mass Spec Gradeの混合物でタンパク質消化が向上

  • 標準的なオーバーナイト消化、非変性条件
  • ペプチド回収率の向上、タンパク質定量精度の向上、再現性の改善
  • トリプシン阻害性不純物に対する耐性

Size

選択製品のカタログ番号: V5071

Please Enquire
This product is discontinued
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
Trypsin/Lys-C Mix, Mass Spec Grade
20μg
Your price:
Change Configuration

質量分析グレードのトリプシン & Lys-C 混合酵素

標準的な消化条件下で、Trypsin/Lys-C Mixは単独のトリプシンに比べてペプチドの消化効率を向上させ、ペプチドの回収率を高めることで、より精度の高いタンパク質定量を実現します。

Trypsin digest illustration.

標準的なトリプシン消化プロトコルによるペプチドおよびタンパク質同定数の増加

Standard overnight trypsin digest schematic 11788ME-W-a
Trypsin LysC overnight digest schematic 11788ME-W-b
Data comparing trypsin and Trypsin/Lys-C digests.
トリプシン単独の消化とTrypsin/Lys-C Mixによる消化におけるペプチドピーク強度を比較した結果、トリプシンによる不完全な消化が明らかとなった。

再現性の向上

Reproducible IgG (Rituximab) digestion with Trypsin Lys-C mix.
Trypsin/Lys-C Mixを使用することで、IgG（リツキシマブ）の消化における再現性が向上した。

トリプシン阻害性不純物への高い耐性

Trypsin LysC digestion results in fewer missed cleavages. 13058MC-W-a
Trypsin LysC increases protein identification compared to trypsin alone. 13058MC-W-b

トリプシン阻害因子の存在下においても、Trypsin/Lys-C Mixは効率的な消化を実現した。モデル系として酵母タンパク質抽出物を使用した。
結論：Trypsin/Lys-Cの使用により、ミスクリーヴ数が減少し、タンパク質の同定数は20%増加した。

プロトコール

Complete Protocol

製品仕様

You are viewing: V5071 Change Configuration

選択製品の構成品内容

Item Part # Size

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507A 1 × 20μg

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

製品仕様

You are viewing: V5072 Change Configuration

選択製品の構成品内容

Item Part # Size

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507B 1 × 100μg

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

製品仕様

You are viewing: V5073 Change Configuration

選択製品の構成品内容

Item Part # Size

Resuspension Buffer

 V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

 V507A 5 × 20μg

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

技術資料

技術記事

類似製品

6 × 5 LC-MS/MS Peptide Reference Mix

LC/MSの機器パフォーマンスモニタリング用ペプチドミックスとフリーソフトウェア

V7491, V7495

Rapid-Digestion Trypsin and Trypsin/Lys-C Kits

最小限のタンパク質消化時間で最大限のデータアウトプット

VA1060, VA1061

rAsp-N, Mass Spec Grade

Cleavage site: N-terminal of Asp. Optimal pH: 6–9.

VA1160

関連製品

Magnetic Proteomics Sample Prep (MPSP) Kit

SP3法によるプロテオームサンプル調製を効率化する磁気ビーズ

CS3325A04

Arg-C, Sequencing Grade

シークエンシンググレードのプロテアーゼ：単独あるいは他のプロテアーゼとの組み合わせて質量分析に

V1881

Asp-N, Sequencing Grade

アスパラギン酸 (Asp)とシステイン酸 (Cys)のＮ末端側のペプチド結合を加水分解するエンドプロテイナーゼ

V1621

Chymotrypsin, Sequencing Grade

Tyr, Phe, TrpのC末端側を優先的に加水分解するセリンプロテアーゼ

V1061, V1062

Let's find the product that meets your needs.

Talk to a Scientist

customer-support-global

Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
India
Japan
/
Republic of Korea
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Portugal
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.