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M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

Part Numbers: M3681, M3682, M3683

M3683_M-MLV-RT-RNase--H---Point-Mutant--50000u_3
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長鎖鋳型 RNA (>5kb) からcDNA を効率的に合成。

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選択製品のカタログ番号: M3681

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M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant
2,500u
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Obtain Higher Yields of cDNA than with the Deletion Mutant

遺伝子操作技術により、MMLV Reverse Transcriptase（RNA依存性DNAポリメラーゼ）からリボヌクレアーゼH活性に関与する部分を点変異させたもので、長い鋳型mRNA（>5kb）からのcDNA合成に最適です。RNase H活性の欠如により、鋳型mRNAの分解が生じる長いcDNA合成などのアプリケーションに使用することができます。M-MLV RT (H+) は分析的、予備的なcDNAアプリケーションで使用できますが、RNase H活性を欠如した逆転写酵素は長いcDNAの調製や完全長cDNAの割合が高いライブラリー作成に威力を発揮します。

  • 5X Reaction Buffer添付：250mM Tris-HCl (pH 8.3 at 25℃), 375mM KCl, 15mM MgCl2, 50mM DTT.
  • ポイントミュータントはデリーションミュータントに比べ高いcDNA収量が得られます。
  • 高い熱安定性により、2次構造に関連した問題を防ぎます。
  • 至的条件の広さ：最適な酵素・基質濃度の幅が広く、より一貫したパフォーマンスを示します。

  • ファーストストランドcDNAの合成
  • プライマーエクステンション
  • RT-PCR

【Ｑ＆Ａ】M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant
  1. Roth, M.J. et al. (1985) J. Biol. Chem. 260, 9326–35.
11143TA

Thermal stability and synthesis of large transcripts. cDNAs were synthesized from 1µg of total human RNA using M-MLV (H–), Point Mutant (Panel A), or SuperScript® II (Panel B) reverse transcriptase at 37, 42 and 50°C. GoTaq® Long PCR amplifications were performed on each transcription reaction using five primer sets, amplifying products from 9.4kb to 1.5kb. Ten microliters of the 9.4, 6.9 and 5.2kb PCR products and 1µl of the 3.2 and 1.5kb PCR products were separated on a 1% agarose gel. Lane M. BenchTop 1kb DNA Ladder (Cat.# G7541).

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製品仕様

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選択製品の構成品内容

Item Part # Size

M-MLV RT, RNase (H–), Point Mutant

 M368A 1 × 2,500 units

M-MLV RT 5X Buffer

 M531A 1 × 1ml

SDS

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分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

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製品仕様

You are viewing: M3682 Change Configuration

選択製品の構成品内容

Item Part # Size

M-MLV RT, RNase (H–), Point Mutant

 M368B 1 × 10,000 units

M-MLV RT 5X Buffer

 M531A 1 × 1ml

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

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製品仕様

You are viewing: M3683 Change Configuration

選択製品の構成品内容

Item Part # Size

M-MLV RT, RNase (H–), Point Mutant

 M368C 1 × 50,000 units

M-MLV RT 5X Buffer

 M531A 5 × 1ml

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

技術資料

サイテーション

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