QuantiFluor® dsDNA System
Sensitive Quantitation of Double-Stranded DNA in Solution
- Specific quantitation of dsDNA
- More sensitive than NanoDrop for low-concentration samples
Catalog Number:
Size
Catalog Number: E2670
Catalog Number: E2671
シンプルで正確な DNA 定量。希釈作業や余分なチューブも不要
QuantiFluor® dsDNA System は溶液中の微量な2本鎖 DNA (dsDNA)を高感度に検出するDNA結合蛍光色素（504nmEx/531nmEm）を含みます。この試薬は正確なDNA定量が不可欠な次世代シークエンシング (NGS) やその他多くの分子生物学アプリケーションに最適です。この色素は1本鎖DNA (ssDNA) 、RNAやタンパク質にほとんど結合しないため、2本鎖DNAの特異的な定量に使用することができます。
【簡易マニュアル】日本語
※日本語マニュアルは英語版を翻訳したものですが、常に更新されるものではありません。最新のマニュアルについては英語版（以下の”プロトコール” 参照）をご覧ください。
【Ｑ＆Ａ】QuantiFluor
Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems
The QuantiFluor® dsDNA Dye offers greater sensitivity over absorbance at 260nm (NanoDrop®) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. 10pg/µl vs. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume).
| Sample* | Assay | |
|---|---|---|
|
QuantiFluor® dsDNA System
|
0.01–200ng/µl
|
0.01–200ng
|
|
Quant-iT™ PicoGreen
|
0.02–200ng/µl
|
0.02–200ng
|
|
Qubit® dsDNA HS
|
0.2–100ng/µl
|
0.2–100ng
|
|
NanoDrop® 2000 Spectrophotometer
|
2–12,000ng/µl
|
2–12,000ng
|
*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.
Better Sensitivity than Quant-iT™ PicoGreen®, Equivalent Linearity
The QuantiFluor® dsDNA System detects as little as 50pg/ml dsDNA in a 96-well microplate assay (1µl of a 10ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.
Proven for DNA Quantitation in Next-Gen Sequencing
Many laboratories have published papers using QuantiFluor® dyes in sequencing applications.
Easy Setup on Microplate or Single-Tube Fluorometers
System includes all the necessary reagents to quickly set up and quantitate dsDNA, and is easy to set up on microplate or single tube fluorometers, including the following Promega fluorometers.
How do fluorescent dyes compare to other quantitation methods?
In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.
UV-Absorbance vs Fluorescence
Fluorescence vs qPCR
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
QuantiFluor® dsDNA Dye |
E258A | 1 × 1ml | |
|
Lambda DNA Standard |
E259A | 1 × 100μg | |
|
20X TE Buffer (pH 7.5) |
E260A | 1 × 25ml | View Product |
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
QuantiFluor® dsDNA Dye, 200X |
E258B | 1 × 100μl | 0.3mM | |
|
Lambda DNA Standard |
E259B | 1 × 10μg | ||
|
20X TE Buffer (pH 7.5) |
E260B | 1 × 5ml | View Product |
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
Resources
技術記事
- Calibrating the Qubit® Fluorometer for QuantiFluor™ dsDNA System
- Calibrating the Qubit® 2.0 Fluorometer for use with the QuantiFluor™ dsDNA System
- Using the QuantiFluor™ dsDNA System and the GloMax® Multi+ Fluorometer with Instinct™ Software
- Evaluation of cfDNA as an early detection assay for dense tissue breast cancer
