QuantiFluor® ssDNA System
Sensitive Quantitation of Single-Stranded DNA
- More sensitive than NanoDrop for low-concentration samples
- Requires less DNA than spectrophotometry
Catalog Number:
Size
Catalog Number: E3190
シンプルで正確なssDNA 定量。希釈作業や余分なチューブは不要です。
QuantiFluor® ssDNA System は溶液中に含まれる少量の 1本鎖 DNA (ssDNA) を高感度に定量できる蛍光色素を含みます。ssDNA の検出や定量は、 ssDNA ウイルスの研究、部位特異的変異導入のための合成 ssDNA プローブの定量、1本鎖 cDNA の分析あるいは DNA メチル化研究で用いるバイサルファイイト処理 DNA の定量など多くの分子生物学研究アプリケーションで有用です。
QuantiFluor® ssDNA System は他法 (1 µg) よりも少量の DNA (200pg) を使用するので、下流のアッセイで使用するサンプルを節約することができます。
Improved Sensitivity vs. Absorbance and Other Fluorescent ssDNA Quantitation Systems
For low-concentration samples, sensitivity of the QuantiFluor® ssDNA System is significantly increased compared to absorbance at 260nm (NanoDrop® method) and Qubit® ssDNA.
The dynamic range of the assay is wider than that of Qubit® ssDNA and equivalent to OliGreen® reagent.
| Sample* | Assay | |
|---|---|---|
|
QuantiFluor® ssDNA System
|
0.2–400ng/µl
|
0.2–400ng
|
|
Quant-iT™ OliGreen
|
0.2–400ng/µl
|
0.2–400ng
|
|
Qubit® ssDNA
|
1–200ng/µl
|
1–200ng
|
|
NanoDrop® 2000 Spectrophotometer
|
2–12,000ng/µl
|
2–12,000ng
|
*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.
ssDNA Quantitation Sensitivity and Assay Linearity
The QuantiFluor® ssDNA System detects as little as 1ng/ml ssDNA in a 96-well microplate assay (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU. Sensitivity is equivalent to Quanti-IT™ OliGreen® reagent.
Easy Setup on Microplate or Single-Tube Fluorometers
The QuantiFluor® ssDNA System includes all the necessary reagents to quickly set up and quantitate single-stranded DNA, and is easy to set up on microplate or single-tube fluorometers. The System is compatible with any fluorometer capable of measuring the appropriate fluorescence excitation and emission spectra. Compatible Promega fluorometers include:
How Do Fluorescent Dyes Compare to Other Quantitation Methods?
In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.
UV-Absorbance vs Fluorescence
Fluorescence vs qPCR
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
20X TE Buffer (pH 7.5) |
E260A | 1 × 25ml | View Product |
QuantiFluor® ssDNA Dye, 200X |
E284A | 1 × 1ml | |
ssDNA Standard |
E288A | 1 × 100μg |
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
