HaloLink™ Resin

Part Numbers: G1912, G1913, G1914, G1915

HaloLink Resin 10ml
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HaloTag® 融合タンパク質を指向性を有した共有結合で捕捉するレジン

  • 共有結合により強く洗浄できるため非特異的なバックグラウンドが最小限
  • 素早く結合するのでタンパク質間相互作用の効率が向上し低濃度タンパク質でも捕捉可能
  • レジン 1ml あたり HaloTag® 融合タンパク質 >7mg を結合

Size

選択製品のカタログ番号: G1912

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HaloLink™ Resin
1.25ml
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Change Configuration

HaloTag® 融合タンパク質を方向性を維持した共有結合により捕捉

HaloLink® ResinはHaloTag®融合タンパク質を固相表面に方向性を維持しながら共有結合で捕捉するためのレジンです。このレジンはSepharose®ビーズにHaloTag®リガンドが固定化されたもので、特異的、迅速にHaloTag®融合タンパク質が結合し、高い結合容量を有しています。本レジンに共有結合したHaloTag®融合タンパク質は溶出されないため、非特異的に結合したタンパク質の洗浄除去を充分に行うことができます。結合速度は非常に迅速で、ビオチン/ストレプトアビジンの速度と同等です。

HaloLink™ Resinは、タンパク質間相互作用(in vivo / in vitro)の検出および分析、固定化されたHaloTag®融合タンパク質の酵素活性測定、酵素によるタンパク質分解をともなった発現タンパク質のワンステップ精製など様々なアプリケーションで使用できます。バクテリア、哺乳動物細胞、セルフリーシステムでHaloTag®融合タンパク質を発現させるベクターが揃います。さらに詳細はこちらをご覧ください: HaloTag® Technology Products

HaloLink®テクノロジーは迅速、便利にHaloTag®融合タンパク質の発現を確認でき、同様にHaloTag® TMR Ligandで蛍光標識した後SDS-PAGEを行うことでHaloLink™ Resinへの固定化効率もモニターできます。ガイドライン: HaloLink™ Resin Technical Manual #TM250.


  • 共有結合による捕捉: 強い洗浄にも耐え得るため、結合したHaloTag®融合タンパク質を失うことなく、非特異的バックグラウンドを最低限に抑えます。
  • 迅速な結合: タンパク質間相互作用の検出を向上させ、低いタンパク質濃度でも結合可能。
  • 方向性を維持した固定: 結合タンパク質から最大限の酵素活性が得られます。
  • 高い結合容量: 沈殿状態のレジン1ml(懸濁状態で4mlに相当)で7mg以上のHaloTag®融合タンパク質を捕捉

  • HaloTag®融合タンパク質の共有結合による固定化

注: リストに記載されているサイズは沈殿したレジンの量です。

【ライセンス】HaloTag®シリーズ

【Q&A】HaloTag® Technology

Detection of protein:protein interactions using HaloLink Resin.

Detection of protein:protein interactions using HaloLink™ Resin. Panels A and B illustrate two approaches to characterizing protein:protein interactions. Panel C shows an analysis of protein:protein interaction.

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BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

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You are viewing: G1913 Change Configuration

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

CC

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

製品仕様

You are viewing: G1914 Change Configuration

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

CC

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

製品仕様

You are viewing: G1915 Change Configuration

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

CC

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

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