QuantiFluor® dsDNA System

シンプルで正確な DNA 定量。希釈作業や余分なチューブも不要

QuantiFluor® dsDNA System は溶液中の微量な2本鎖 DNA (dsDNA)を高感度に検出するDNA結合蛍光色素（504nmEx/531nmEm）を含みます。この試薬は正確なDNA定量が不可欠な次世代シークエンシング (NGS) やその他多くの分子生物学アプリケーションに最適です。この色素は1本鎖DNA (ssDNA) 、RNAやタンパク質にほとんど結合しないため、2本鎖DNAの特異的な定量に使用することができます。

【簡易マニュアル】日本語
※日本語マニュアルは英語版を翻訳したものですが、常に更新されるものではありません。最新のマニュアルについては英語版（以下の”プロトコール” 参照）をご覧ください。

【Ｑ＆Ａ】QuantiFluor

Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems

The QuantiFluor® dsDNA Dye offers greater sensitivity over absorbance at 260nm (NanoDrop®) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. 10pg/µl vs. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume).

	Sample*	Assay
QuantiFluor® dsDNA System	0.01–200ng/µl	0.01–200ng
Quant-iT™ PicoGreen	0.02–200ng/µl	0.02–200ng
Qubit® dsDNA HS	0.2–100ng/µl	0.2–100ng
NanoDrop® 2000 Spectrophotometer	2–12,000ng/µl	2–12,000ng

*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.

Better Sensitivity than Quant-iT™ PicoGreen®, Equivalent Linearity

The QuantiFluor® dsDNA System detects as little as 50pg/ml dsDNA in a 96-well microplate assay (1µl of a 10ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.

Proven for DNA Quantitation in Next-Gen Sequencing

Many laboratories have published papers using QuantiFluor® dyes in sequencing applications.

View Sample Citations

Easy Setup on Microplate or Single-Tube Fluorometers

System includes all the necessary reagents to quickly set up and quantitate dsDNA, and is easy to set up on microplate or single tube fluorometers, including the following Promega fluorometers.

Quantus Nucleic Acid Quantification Fluorometer

Quantus™ Fluorometer

(Use Blue Channel Ex 460nm, Em 515–575nm)

Discover Nanodrop Spectrophotometer DNA Extraction

GloMax® Discover

Explorer DNA Purification & Quantification Kit

GloMax® Explorer

How do fluorescent dyes compare to other quantitation methods?

In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.

UV-Absorbance vs Fluorescence

Fluorescence vs qPCR

プロトコール

Complete Protocol

QuantiFluor® dsDNA System Technical ManualPDF (1765 KB) – English

Quick Protocols

QuantiFluor® dsDNA System Quick Protocol FB197PDF (732 KB) – English

選択製品の構成品内容

Item	Part #	Size	Available Separately
QuantiFluor® dsDNA Dye	E258A	1 × 1ml
Lambda DNA Standard	E259A	1 × 100μg
20X TE Buffer (pH 7.5)	E260A	1 × 25ml	View Product

SDS

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分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

パテントおよび免責条項

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.